Specific Identification of Fraction-I-Positive Pasteurella Pestis Colonies on Antiserum-Agar Plates

Albizo, Johnnie M.; Surgalla, Michael J.

doi:10.21236/ad0835494

Cited by 2 publications

(3 citation statements)

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“…Because the antiserum-agar plate test is specific for P. pestis when specific antiplague serum is incorporated into various nutrient agar media, it may become unnecessary to conduct all of the additional tests known for confirming P. pestis. This view is supported by results obtained both in this and in an earlier study (1). If, however, it is considered necessary to obtain isolates for performing complete characterizations, or for use in research, the present method readily permits it.…”

Section: Discussionsupporting

confidence: 78%

Use of the Antiserum-Agar Plate Technique for Specific Identification and Isolation of Pasteurella pestis

Albizo¹,

Surgalla²

1968

Applied Microbiology

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Pasteurella pestis colonies were specifically identified on antiserum-agar plates used for primary culture of tissues from experimentally infected guinea pigs. Both selective and nonselective antiserum-agar plates were used to identify P. pestis from guinea pigs kept at 22 C for periods up to 4 days after death from plague. Colonies identified as P. pestis on selective and nonselective antiserum-agar plates, by the appearance of precipitin rings following brief chloroform vapor treatment, remained viable and were subsequently purified on nonselective antiserum-agar plates. Isolates obtained in this manner were uniformly lethal when injected into mice and guinea pigs, and conformed to standard laboratory criteria for P. pestis. P. pestis was identified on selective antiserum-agar plates from the spleens of all guinea pigs killed by the isolates, and from a large majority of the mice. The practical value and confirmative nature of the method were demonstrated. Recently, we reported a serological technique (1) for specifically identifying fraction I-positive Pasteurella pestis colonies on nutrient agar plates

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Section: Discussionsupporting

confidence: 78%

Use of the Antiserum-Agar Plate Technique for Specific Identification and Isolation of Pasteurella pestis

Albizo¹,

Surgalla²

1968

Applied Microbiology

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“…GAD GIL et al (1966) verified this finding and, with some foresight, noted that elongation could occur if the synthesis of DNA was selectively inhibited. The possibility that stasis reflects primary damage to the cytoplasmic membrane, suggested by SURGALLA et al (1968) and by results obtained with vwa+ cells of Y. pseudotuberculosis (BRUBAKER, 1967), was not substantiated by BRUBAKER and YANG (1971) and YANG and BRU-BAKER (1971 a) who failed to detect significant differences between dividing and static cells with respect to permeation of L-isoleucine, oxygen uptake, or release of preloaded 32P. The cytoplasmic membranes of static cells could not be distinguished from those of dividing organisms by inspection of thin sections with the electron microscope (YANG et aI., 1971 b).…”

Section: Virulence or V And W Antigensmentioning

confidence: 99%

“…Congo red agar did not permit significant pigmentation of isolates of Salmonella, Shigella Klebsiella, Pseudomonas, Proteus, Bacillus, or Staphylococcus although an intense reaction was observed with certain yeasts and members of the family Micrococcaceae. SURGALLA et al (1968) showed that a marked population-shift favoring pgm-cells occurs at 26° C in broth cultures during the death phase due to accelerated loss of pgm+ organisms. This loss could be eliminated by reducing the terminal pH to neutrality with HCl or by maintaining electrolyte balance with Na+ rather than K+.…”

Section: Pigmentationmentioning

confidence: 99%

Current Topics in Microbiology and Immunology

1972

Current Topics in Microbiology and Immunology

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Published by Springer-Verlag Price : 129.95 € Hardcover Pages : 255, Vol 340, 2010 ISBN : 978-3-642-03857-0 DOI : 10.1007 Reviewed by Rajendra Kumar and Rupali Sharma "Current topics in microbiology and immunology: Immunological Synapse" Reviewed by Rajendra Kumar and Rupali Sharma.Published by Springer pp 255, Price 129.95 €(Hardcover).The science of immunology has seen outsized transformation from a medicine named vaccination to a contemporary applied science focused on versatile development in molecular medicine. Fundamental principles of immunology along with their clinical applications provide an exciting platform for specialization. Immune system is able to engender variety of cells and molecules capable of recognizing and removing infinite number of foreign intruders. Functioning of immune system is dependent on a reliable cell-cell communication, which forms the basis of phenomena of Immunological Synapse.Current volume is an attempt of an additional step toward understanding the immunological synapse. It has the essence of most consensual volume, as editors invited a number of key researchers in the field, to provide perspectives and current understanding of the molecular process in the wake of the formation and function of the immunological synapse. This book targets the professionals as well as the medical or bioscience students by emphasizing on the recent advancement in immunological synapse.In first chapter, Michael L. Dustin has enlighten the function of immunological synapse and have explained the basic features of molecular interactions in the synapse using the lipid bi-layer on glass surface model. Further he has also explained that how this model along with fluorescent microscopy, can be exploited for the measurement of 2-D affinity and kinetics in cell-cell contact.Junsang Doh and Matthew F. Krummel have contributed the second chapter. They have reviewed the pattern of cell-cell communication in T lymphocytes and antigen presenting cells and extrapolated it into the framework of immune system as a whole. Here, the authors have suggested various patterns of cell-cell communication in the immune system and have described that how each type of pattern suffice the need of an efficient communication in the system. Yolanda R. Carrasco has presented his perspective on B-cell synapse by spreading-contraction response theory of B cell activation in the succeeding chapter. Here, authors have suggested that immunological synapse is crucial for increasing the sensibility of B cells to antigen. The authors have discussed the role of single cell imaging techniques like real-time confocal microscopy and total interference reflection fluorescence microscopy in the study of the molecular mechanisms beneath B cell activation.Since it is crucial to control the immune response for maintaining the self-tolerance, the inhibitory immune synapse comes into the picture. This view has been presented by Philipp Eissmann and Daniel M. Davis. Here, the authors have discussed the role of supra-molecular assemblies to co...

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Specific Identification of Fraction-I-Positive Pasteurella Pestis Colonies on Antiserum-Agar Plates

Cited by 2 publications

References 9 publications

Use of the Antiserum-Agar Plate Technique for Specific Identification and Isolation of Pasteurella pestis

Use of the Antiserum-Agar Plate Technique for Specific Identification and Isolation of Pasteurella pestis

Current Topics in Microbiology and Immunology

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