Specific identification of Gallibacterium by a PCR using primers targeting the 16S rRNA and 23S rRNA genes

Tissue distribution of haemolyticGallibacterium anatisisolates in laying birds with reproductive disorders

“…The weak and unreliable reactions observed for some of the phenotypic identification tests add to these uncertainties (Christensen et al, 2003). Diagnostic PCR assays were therefore developed to complement these diagnostic methods (Bojesen et al, 2007) and were found very useful in confirming the classification of the isolates. The genetic diversity assessed by AFLP analysis of 83 isolates confirmed previous indications of high genetic relatedness among isolates from the same flock (Bojesen et al, 2003a).…”

“…All isolates suggestive of Gallibacterium spp. were investigated according to Bojesen et al (2007). Using this polymerase chain reaction (PCR), two fragments of approximately 790 base pairs (bp) and 1080 bp were expected for strains of the genus Gallibacterium.…”

Assessing pathogenicity ofGallibacterium anatisin a natural infection model: the respiratory and reproductive tracts of chickens are targets for bacterial colonization

“…Furthermore, Gallibacterium anatis has two biovars: G. anatis haemolytica and G. anatis anatis. Bacterial identification can be performed on the basis of phenotypic properties, but in addition G. anatis-specific polymerase chain reaction (PCR), quantitative PCR, fluorescent in situ hybridization and matrix-assisted laser desorption/ionization time-offlight mass spectrometry (MALDI-TOF MS) techniques have been shown to be promising as a means of accurate diagnosis (Bojesen et al, 2003a(Bojesen et al, , 2007Alispahic et al, 2011;Huangfu et al, 2012). Also, MALDI-TOF MS is well established for the characterization of Gallibacterium isolates into existing species, in agreement with multi-locus sequence typing (Alispahic et al, 2011).…”

Genetic diversity ofGallibacteriumisolates from California turkeys

“…An amplicon of intermediate size indicating the existence of a third size ribosomal operon has been described previously by Christensen et al . (2003); however, based on the previous and present results this operon does not seem to occur very frequently in Gallibacterium (Bojesen et al ., 2007). The internal amplification control based on the rpoB gene was positive in all samples.…”

“…All isolates were subjected to phenotypic characterization in accordance with Bisgaard (1982) and Christensen et al . (2003), and genotypic identification by the recently described Gallibacterium -specific polymerase chain reaction (PCR) (Bojesen et al ., 2007). This PCR is based on primers targeting the 16S and 23S rRNA genes, generating two or three amplicons depending on the number of different ribosomal operons present.…”