Specific IgE antibodies in atopic eczema

Hoffman, Donald R.; Yamamoto, Franklin Y.; Geller, B.D.; Haddad, Zack H.

doi:10.1016/0091-6749(75)90145-1

Cited by 113 publications

(38 citation statements)

References 11 publications

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“…As can be seen from these data, a direct demonstrate the age and sex distribution of our patients and the correlation exists between these events ( r = 0.7165, P < 0.01). An e l e v a t e d serum IgE c o n c e n t r a t i o n in 83% of our p a t i e n t s confirms t h e s t u d i e s of o t h e r s (3,(5)(6)(7)(8)(9). w h o r e p o r t an increased frequency of e l e v a t e d serum I g E in p a t i e n t s w i t h a t o p i c d e r m a t i t i s .…”

Section: Skin Testingsupporting

confidence: 66%

“…Although the pathogenesis is unknown, the strong association of atopic dermatitis, asthma, hay fever, and family history of atopic disease suggests that immunologic mechanisms are frequently involved. Elevated serum IgE levels have been reported frequently in atopic dermatitis, (1, [6][7][8]10) and the concentration of IgE appears to be correlated with the severity of the dermatitis (6,7). Further, Hoffman et al (5,6) have recently demonstrated specific IgE to a wide variety of antigen in patients with eczema.…”

Section: Speculationmentioning

confidence: 99%

“…Elevated serum IgE levels have been reported frequently in atopic dermatitis, (1,(6)(7)(8)10) and the concentration of IgE appears to be correlated with the severity of the dermatitis (6, 7). Further, Hoffman et al (5,6) have recently demonstrated specific IgE to a wide variety of antigen in patients with eczema.The present study was undertaken to evaluate serum IgE levels in a selected group of pediatric patients seen in an allergy clinic to determine the presence or absence of allergen-specific IgE and to examine the relation of atopic dermatitis to inhalant and food allergies. …”

mentioning

confidence: 99%

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Serum Immunoglobulin E Concentrations and Radioallergosorbent Tests in Children with Atopic Dermatitis

1976

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ExtractSerum IgE concentrations and the presence of allergen-specific IgE were determined in a series of 23 children with atopic dermatitis. In this group 83% had significantly elevated serum levels of IgE, 91% had coexistent respiratory allergy, 70% had radioallergosorbent test (RAST) evidence of pollen hypersensitivity, and 43% gave a history and demonstrated a RAST score consistent with milk or egg hypersensitivity. In patients with eczema a significant proportion of the elevated serum IgE appears to be antigen specific. SpeculationThe mechanisms involved in the pathogenesis of atopic dermatitis remain unknown. Elevated serum IgE and the presence of antigen specific IgE suggest a causal relationship of atopic eczema with immediate hypersensitivity. Alternatively, the disease process may ,have a marked stimulatory effect on the specific IgE response. The precise nature of this association remains to be determined.Atopic dermatitis or eczema has been defined as an allergic dermatosis which is characterized by erythema, intense itching, and vesiculation (3). Although the pathogenesis is unknown, the strong association of atopic dermatitis, asthma, hay fever, and family history of atopic disease suggests that immunologic mechanisms are frequently involved. Elevated serum IgE levels have been reported frequently in atopic dermatitis, (1,(6)(7)(8)10) and the concentration of IgE appears to be correlated with the severity of the dermatitis (6, 7). Further, Hoffman et al. (5,6) have recently demonstrated specific IgE to a wide variety of antigen in patients with eczema.The present study was undertaken to evaluate serum IgE levels in a selected group of pediatric patients seen in an allergy clinic to determine the presence or absence of allergen-specific IgE and to examine the relation of atopic dermatitis to inhalant and food allergies. SUBJECTS AND METHODS PATIENT POPULATIONThe patient population consisted of 23 children ranging in age from I to 15 years with varying degrees of symptomatic atopic dermatitis seen at the Georgetown University Allergy-lmmunology-Respiratory Disease (AIR) Clinic (Table 1). There were 10 males and 13 females in the study group. In order to examine the relationships of age and IgE, the patients were divided into four age categories: 9 were 1-4 years of age, 4 were 5-8 years of age, 6 were 9-12 years of age, and 4 were 13-15 years of age. CLASSIFICATION OF DISEASEAtopic dermatitis was classified as mild, moderate, or severe according to the extent of the eruption at the time serum samples were obtained. 'Dermatitis of limited distribution with few or no active lesions was classified as "mild." Dermatitis limited to the face, neck, or flexor surfaces of the extremities with limited erythema and vesidulation was "moderate." Extensive involvement of the trunk and extensor surfaces, severe lichenification, extensive erythema, and vesiculation or any of these in combination were classified as "severe."For the purpose of this study, allergic rhinitis or asthma was designated as present (+) or a...

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Section: Skin Testingsupporting

confidence: 66%

Section: Speculationmentioning

confidence: 99%

mentioning

confidence: 99%

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Serum Immunoglobulin E Concentrations and Radioallergosorbent Tests in Children with Atopic Dermatitis

1976

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“…In atopic dermatitis, elevated serum IgE is observed in about 80% of patients, 23,24) and blood eosinophil counts are also elevated. Activated eosinophils are accumulated and deposition of eosinophil cationic protein released from the activated eosinophils is demonstrated in skin lesions.…”

Section: Discussionmentioning

confidence: 99%

Establishment of Allergic Dermatitis in NC/Nga Mice as a Model for Severe Atopic Dermatitis

Gao

Nakamura

Fuseda

et al. 2004

Biological & Pharmaceutical Bulletin

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Mite antigen has been suggested to play important roles in the onset and/or development of atopic dermatitis, and mite antigen-induced dermatitis models appear beneficial for the basic study of atopic dermatitis. In the present study therefore, we attempted to establish an allergic dermatitis model in mice using Dermatophagoides farinae crude extract as an antigen. Mite antigen solution at a concentration of 1 or 10 mg/ml was painted 5 times repeatedly at an interval of 7 d onto the ear of NC/Nga or BALB/c mice with or without simultaneous tape-stripping. Apparent biphasic ear swelling was observed after the 4th and 5th antigen applications in both strains of mice treated with 10 mg/ml of antigen solution. Thickening of the epidermis, fibrosis of the dermis, and the accumulation of inflammatory cells were also observed after the 5th application. The inflammatory changes were more evident in NC/Nga mice than in BALB/c mice and potentiated by tape-stripping. The ear swelling was accompanied by increased serum IgE, increased expression of interleukin-4 mRNA and decreased expression of interferon-g g mRNA in cervical lymph nodes and ears. These results indicate that ear swelling caused by repeated mite antigen application with simultaneous tape-stripping has a Th2-dominant background and that the inflammatory responses are expressed more potently in NC/Nga mice than in BALB/c mice. The dermatitis caused by mite antigen in NC/Nga mice appears to be a useful model for the basic study of atopic dermatitis.

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“…4,5) We attempted to prepare rats with high IgE as a model of patients with allergic diseases. Rats were activated using 2,4-dinitrophenylated ascaris extract (DNP-As) as antigen and were immunized with killed Bordetella pertussis as adjuvant, which is reported to enhance IgE antibody formation in rats and mice.…”

mentioning

confidence: 99%

Wistar Strain Rats as the Model for IgE Antibody Experiments.

Hirano

Kawasaki

Miyataka

et al. 2001

Biological & Pharmaceutical Bulletin

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In recent years, an increase has been reported globally in the number of cases of allergic disease. Japan is no exception, and allergic diseases including cedar pollenosis, atopic diseases, and extrinsic asthma have raised grave social concern.2) These allergic symptoms are generally classified as the type I allergy and well-known immediate-type reactions. They are also known to be caused by the release of mediator from target cells such as mast cells and basophils following exposure to an antigen. When an antigen enters the body, it is phagocytosed by macrophages, which then present it to T cells. Differentiated (proliferated) T cells present the antigen to B cells. After differentiation to antibody-forming cells by the action of cytokines such as interleukin (IL)-4, B cells produce IgE antibody.3) An increase in plasma IgE level is observed in about 80% of patients with atopic dermatitis and this is closely related to their long-term serious symptoms. 4,5) We attempted to prepare rats with high IgE as a model of patients with allergic diseases. Rats were activated using 2,4-dinitrophenylated ascaris extract (DNP-As) as antigen and were immunized with killed Bordetella pertussis as adjuvant, which is reported to enhance IgE antibody formation in rats and mice. [6][7][8] The amount of IgE antibody formed and its change over time were then studied in the rats. MATERIALS AND METHODSAnimals Six-week-old male and female, Sprague-Dawley (SD), Donryu, and Wistar strain rats were kindly supplied by Japan SLC Inc. The animals were used in the experiments after preliminary feeding for one week.Reagents DNP-As and killed B. pertussis were purchased from LSL Co., Ltd., and Chiba Serum Research Laboratory, respectively. Morinaga Institute of Biological Sciences kindly donated enzyme-linked immunosorbent assay (ELISA) kits (Morinaga Rat IgE ELISA Kit).Immunization Immunization of rats was performed with reference to the method of Matsuura et al.9) DNP-As (1 mg per animal) was dissolved in physiological saline 0.1 ml and the solution was mixed with killed B. pertussis 0.5 ml of containing 10 10 cells. The mixture was subcutaneously injected in the paws of each rat for early immunization. Five days after early immunization, a solution of DNPAs 0.5 mg in physiological saline 0.25 ml was injected into the back muscle of each rat to produce additional immunization.Blood Sampling and Measurement Blood was drawn from the jugular vein of each rat 0, 5, 10, 20, and 25 d after additional immunization. The day of additional immunization was taken as day 0. The blood was centrifuged at 4°C for 10 min at 700ϫg and the plasma obtained was used as the sample. The plasma was frozen at Ϫ20°C and stored until the time of IgE determination. The amount of IgE was determined by the EIA sandwich method using ELISA. The sample was placed in a plate coated with solid phase-bound anti-rat IgE monoclonal antibody and the plate was allowed to stand at room temperature for 4 h, during which the antibody formed a complex with rat IgE. After washing each...

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Specific IgE antibodies in atopic eczema

Cited by 113 publications

References 11 publications

Serum Immunoglobulin E Concentrations and Radioallergosorbent Tests in Children with Atopic Dermatitis

Serum Immunoglobulin E Concentrations and Radioallergosorbent Tests in Children with Atopic Dermatitis

Establishment of Allergic Dermatitis in NC/Nga Mice as a Model for Severe Atopic Dermatitis

Wistar Strain Rats as the Model for IgE Antibody Experiments.

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