Specific IgE determination by RAST compared with skin and provocation tests in allergy diagnosis with birch pollen, timothy pollen and dog epithelium allergens

Ahlstedt, S.; Eriksson, N. E.; Lindgren, S.; Röth, Alexander

doi:10.1111/j.1365-2222.1974.tb01370.x

Cited by 66 publications

(30 citation statements)

References 6 publications

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“…35 patients had negative reactions to all allergens used. 34 patients had earlier been sub jected to immunotherapy but our previous study did not indicate any significant influence on the results [5].…”

Section: Patientsmentioning

confidence: 96%

“…Economical considerations have also to be included before the RAST can be more generally recommended to replace the conventional biologic test methods in the elaboration of a complicated allergy diagno sis. In a previous study we suggested that combinations of RAST results and the patients' case histories in a score system could be used for screening allergic patients, thus making provocation tests necessary only in a limited number of cases [5]. The results of applying this approach to the pre sent patient material will be discussed in a further paper.…”

Section: Discussionmentioning

confidence: 99%

“…In a previous study with selected adult allergic patients, we reported an overall cor relation of 84% between RAST and PT for birch pollen, timothy pollen and dog epithel ium allergens [5], In the present study a large number of patients and 16 different al lergens were used in an attempt to assess the possibility of replacing the time-consuming and potentially hazardous in vivo diagnostic methods with the more conveniant and easi ly available RAST (Phadebas RAST®). The RAST results were compared with the results of ST and PT in adult patients with asthma and/or allergic rhinitis.…”

Section: Introductionmentioning

confidence: 99%

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Diagnosis of Reaginic Allergy with House Dust, Animal Dander and Pollen Allergens in Adult Patients

Ne¹,

Ahlstedt²,

Belin³

1976

Int Arch Allergy Immunol

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RAST analyses were performed with house dust, animal dander and eleven pollen allergens in 410 adult patients with asthma and/or rhinitis and the findings were compared with skin test and provocation test results. The overall correlation between 1,316 provocation tests and RAST was 78% and the correlation between 1,778 skin tests and RAST was 66%. The relationships between RAST and in vivo tests differed considerably from allergen to allergen. No significant differences were found between various age groups. According to the in vivo tests the RAST showed more often positive results in patients with pronounced allergy than in patients with low-grade allergy. Higher RAST classes were most often combined with positive in vivo tests. It is concluded that in cases with RAST classes 2–4, a reasonably well-established allergy diagnosis can be reached without the inclusion of additional in vivo tests. In other cases, however, application of further diagnostic methods is required.

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Section: Patientsmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Diagnosis of Reaginic Allergy with House Dust, Animal Dander and Pollen Allergens in Adult Patients

Ne¹,

Ahlstedt²,

Belin³

1976

Int Arch Allergy Immunol

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“…Ten patients (age range 22-58 years) with exogenous seasonal asthma were investigated, Manifest exogenous atopic allergic asthma was considered when the sum score for history of positive symptomatology (max score 3), SPT (max score 3) and positive RAST titres (max score 3) was at least 5 (2). At the time of blood sampling, all patients had been free from all drugs, including oral contraceptives, for at least 10 days.…”

Section: Blood Donorsmentioning

confidence: 99%

Defective Serotonin (5‐HT) Transport Mechanism in Platelets from Patients with Endogenous and Allergic Asthma

Malmgren

Grubbströum

Olsson

et al. 1982

Allergy

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The kinetics of the 5-HT-uptake by platelets in platelet-rich plasma (PRP) and PRP diluted with autologous platelet-poor plasma (PRP/PPP) were determined in normal subjects, patients with asymptomatic exogenous asthma and those with symptomatic endogenous asthma.In normal subjects the 5-HT-uptake by platelets strictly obeyed Michaelis-Menten's kinetics. In patients with asymptomatic exogenous asthma, the active 5-HT-transport was moderately distorted in PRP and severely altered in PRP/PPP. Patients with symptomatic endogenous asthma exhibited already in PRP a dramatic transport disturbance.The alteration of the active 5-HT-transport was characterized by increasing uptake inhibition by rising 5-HT concentrations in plasma. This phenomenon could be due to changes in the platelet environment, defects in the platelet membrane or intrinsic platelet disorders. It is concluded that asthmatics have a genetically or later acquired platelet disorder, which is more clearly observed in patients with symptomatic endogenous asthma than in those with asymptomatic exogenous asthma.Kcy words: endogenous and exogenous asthmatics; platelets; serotonin transport. Accepted for publicdon 22 August 1981 CLINICAL ASPECTSThe 5-€IT (serotonia) uptake by platelets from patients with nstbma WPB studied. J n patients with symptomatic endogenous nsthma, the 5-HT-uptake by platelets WM severely impaired, and aUghtly disturbed in platelets from patients with psymptomntic exogenous M -.Serum and whole blood from asthmatic patients haa proved to contain higher amounts of 5-HT than that of normal subjects, and elevated levels of 5-HT-metaboliterr ouch M kynureniae has been demonstrated in such patients. Furthermore, tryptophan load on psthmaties leads to abnormally higb urinary excretion of xnnthurenic acid.As tryptophan ia the main precursor in 5-HT synthesis, a reduced tryptophan intake might thus be of importance.It is reported that patients with allergic asthma have higher blood levels of 5-HT than normal persons (3, 9) and excrete more 5-HT metabolites in urine (21). Asthmatic patients also seem to be more sensitive to administration of 5-HT than healthy subjects (4). Since the circulating platelet is involved in the uptake, secretion, and transport of 5-HT (15), we have previously found reason to study platelets from asthmatic patients. It was demonstrated that such platelets have a reduced capacity to accumulate 5-HT (11) and a disturbed active 5-HT uptake mechanism (12).The experimental conditions, under which the 5-HT binding and uptake by platelets should be performed, have been further elaborated, as has the mathematical treatment of data for detecting abnormalities in kinetic pat-0 1054538/82/0 1002%l1 $02.50/0 0 1982 Munksgaard, Copenhagen

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“…In order to make use of the quantitative information available from the ST and the RAST results as well as to some extent from the CH, a scoring system suggested in a previous paper [4] was employed in the present investigation. It was found that when two diagnostic methods were used to gether, a 100% agreement with PT could be obtained in cases with maximal scores (scores of 6).…”

Section: Discussionmentioning

confidence: 99%

Diagnosis of Reaginic Allergy with House Dust, Animal Dander and Pollen Allergens in Adult Patients III.

Ne¹

1977

Int Arch Allergy Immunol

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Case histories alone and combined with the results of skin tests and/or the radioallergosorbent test (RAST) were compared with the results of provocation tests in 397 patients with asthma or allergic rhinitis. Case histories often turned out to be doubtful. The agreement between negative histories and negative provocation tests was 77% and that between positive histories and positive provocation tests 64%. When case histories were supported by concordant skin test or RAST results, the agreement with provocation tests increased to about 90%. When a scoring system for the combinations of different diagnostic methods was employed, a 100% agreement with provocation tests was obtained for cases with high scores. It is concluded that by using the case history in conjunction with RAST and/or skin test results, a reliable diagnosis can be achieved, eliminating the necessity of provocation tests.

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Specific IgE determination by RAST compared with skin and provocation tests in allergy diagnosis with birch pollen, timothy pollen and dog epithelium allergens

Cited by 66 publications

References 6 publications

Diagnosis of Reaginic Allergy with House Dust, Animal Dander and Pollen Allergens in Adult Patients

Diagnosis of Reaginic Allergy with House Dust, Animal Dander and Pollen Allergens in Adult Patients

Defective Serotonin (5‐HT) Transport Mechanism in Platelets from Patients with Endogenous and Allergic Asthma

Diagnosis of Reaginic Allergy with House Dust, Animal Dander and Pollen Allergens in Adult Patients III.

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