Specific Incorporation of Polyunsaturated Fatty Acids into the sn-2 Position of Phosphatidylglycerol Accelerates Photodamage to Photosystem II under Strong Light

Jimbo, Haruhiko; Yuasa, Kei; Takagi, Kensuke; Hirashima, Takashi; Keta, Sumie; Aichi, Makiko; Wada, Hajime

doi:10.3390/ijms221910432

Cited by 7 publications

(5 citation statements)

References 54 publications

(118 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2022 ). The FAs incorporated from the medium reached 20 mol% of the total FAs in PG ( Jimbo et al. 2021b ), which was as little as 2 mol% of the cellular FAs.…”

Section: Discussionmentioning

confidence: 97%

“…2020 ). Under strong light, the supplemented free fatty acids were specifically incorporated into the sn -2 position of phosphatidylglycerol (PG), which is the major phospholipid in cyanobacterial cells, through the turnover of PG ( Jimbo et al. 2021b , Kojima et al.…”

Section: Discussionmentioning

confidence: 99%

“…2021a ). Fatty acids bound to glycerolipids were methyl-esterified by the KOH–methanol as described previously ( Jimbo et al. 2021b ).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

High Myristic Acid in Glycerolipids Enhances the Repair of Photodamaged Photosystem II under Strong Light

Kurima,

Jimbo,

Fujihara

et al. 2024

Plant and Cell Physiology

Self Cite

View full text Add to dashboard Cite

Cyanobacteria inhabit areas with a broad range of light, temperature, and nutrient conditions. The robustness of cyanobacterial cells, which can survive under different conditions, may depend on the resilience of photosynthetic activity. Cyanothece sp. PCC 8801 (Cyanothece), a freshwater cyanobacterium isolated from a Taiwanese rice field, had a higher repair activity of photodamaged photosystem II (PSII) under intense light than Synechocystis sp. PCC 6803 (Synechocystis), another freshwater cyanobacterium. Cyanothece contains myristic acid (14:0) as the major fatty acid at the sn-2 position of the glycerolipids. To investigate the role of 14:0 in the repair of photodamaged PSII, we used a Synechocystis transformant expressing a T-1274 encoding a lysophosphatidic acid acyltransferase (LPAAT) from Cyanothece. The wild-type and transformant cells contained 0.2 and 20.1 mol% of 14:0 in glycerolipids, respectively. The higher content of 14:0 in the transformants increased the fluidity of the thylakoid membrane. In the transformants, PSII repair was accelerated due to an enhancement in the de novo synthesis of D1 protein, and the production of singlet oxygen (1O2), which inhibited protein synthesis, was suppressed. The high content of 14:0 increased transfer of light energy received by phycobilisomes to PSI and CP47 in PSII and the content of carotenoids. These results indicated that an increase in 14:0 reduced 1O2 formation and enhanced PSII repair. The higher content of 14:0 in the glycerolipids may be required as a survival strategy for Cyanothece inhabiting a rice field under direct sunlight.

show abstract

“…2022 ). The FAs incorporated from the medium reached 20 mol% of the total FAs in PG ( Jimbo et al. 2021b ), which was as little as 2 mol% of the cellular FAs.…”

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

High Myristic Acid in Glycerolipids Enhances the Repair of Photodamaged Photosystem II under Strong Light

Kurima,

Jimbo,

Fujihara

et al. 2024

Plant and Cell Physiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…PSII is located in the thylakoid membrane, and PSII-associated galactolipids were found to undergo deacylation remodeling during the PSII repairing process (Jimbo and Wada 2023 ). Moreover, the effect of eFFAs on the activity of PSII under strong light in Synechocystis 6803 has also been investigated by Jimbo et al ( 2020 , 2021b ): while C12:0 accelerates the photoinhibition of PSII by inhibiting its repair and the de novo synthesis of D1 (key subunit of PSII), and C18:3 has an impact on both the repair of and photodamage to PSII, C16:0, and C18:0 enhance PSII repair, by accelerating the de novo synthesis of D1 as well as mitigating photoinhibition of PSII. The amount of unsaturated membrane lipids was also reported to fluctuate as a protective response against osmotic stress (Mikami and Murata 2003 , Yang et al 2020 ).…”

Section: Lipid Turnovermentioning

confidence: 98%

Incorporation, fate, and turnover of free fatty acids in cyanobacteria

Kahn

Oliveira

Cuau

et al. 2023

FEMS Microbiology Reviews

View full text Add to dashboard Cite

Fatty acids are important molecules in bioenergetics and also in industry. The phylum Cyanobacteria consists of a group of prokaryotes that typically carry out oxygenic photosynthesis with water as an electron donor and use carbon dioxide as a carbon source to generate a range of biomolecules, including fatty acids. They are also able to import exogenous free fatty acids and direct them to biosynthetic pathways. Here, we review current knowledge on mechanisms and regulation of free fatty acid transport into cyanobacterial cells, their subsequent activation and use in the synthesis of fatty acid-containing biomolecules such as glycolipids and alka(e)nes, as well as recycling of free fatty acids derived from such molecules. This review also covers efforts in the engineering of such cyanobacterial fatty acid-associated pathways en route to optimized biofuel production.

show abstract

“…The n-3 PUFAs, such as ALA, EPA and DHA, and the n-6 PUFAs, such as arachidonic acid (AA), LA, γ-linolenic acid (GLA), dihomo-γ-linolenic acid (DGLA) and adrenic acid (AdA), serve as precursors for oxylipin formation [ 34 ]. As mentioned, dietary intake or supplementation with n-3 PUFAs leads to the incorporation of these n-3 PUFAs into phospholipids, usually at the SN 2 position [ 38 ].…”

Section: Enzymatic Metabolism Of Ala To Oxylipinsmentioning

confidence: 99%

The Role of α-Linolenic Acid and Its Oxylipins in Human Cardiovascular Diseases

Cambiaggi

Chakravarty

Noureddine

et al. 2023

IJMS

View full text Add to dashboard Cite

α-linolenic acid (ALA) is an essential C-18 n-3 polyunsaturated fatty acid (PUFA), which can be elongated to longer n-3 PUFAs, such as eicosapentaenoic acid (EPA). These long-chain n-3 PUFAs have anti-inflammatory and pro-resolution effects either directly or through their oxylipin metabolites. However, there is evidence that the conversion of ALA to the long-chain PUFAs is limited. On the other hand, there is evidence in humans that supplementation of ALA in the diet is associated with an improved lipid profile, a reduction in the inflammatory biomarker C-reactive protein (CRP) and a reduction in cardiovascular diseases (CVDs) and all-cause mortality. Studies investigating the cellular mechanism for these beneficial effects showed that ALA is metabolized to oxylipins through the Lipoxygenase (LOX), the Cyclooxygenase (COX) and the Cytochrome P450 (CYP450) pathways, leading to hydroperoxy-, epoxy-, mono- and dihydroxylated oxylipins. In several mouse and cell models, it has been shown that ALA and some of its oxylipins, including 9- and 13-hydroxy-octadecatrienoic acids (9-HOTrE and 13-HOTrE), have immunomodulating effects. Taken together, the current literature suggests a beneficial role for diets rich in ALA in human CVDs, however, it is not always clear whether the described effects are attributable to ALA, its oxylipins or other substances present in the supplemented diets.

show abstract

Specific Incorporation of Polyunsaturated Fatty Acids into the sn-2 Position of Phosphatidylglycerol Accelerates Photodamage to Photosystem II under Strong Light

Cited by 7 publications

References 54 publications

High Myristic Acid in Glycerolipids Enhances the Repair of Photodamaged Photosystem II under Strong Light

High Myristic Acid in Glycerolipids Enhances the Repair of Photodamaged Photosystem II under Strong Light

Incorporation, fate, and turnover of free fatty acids in cyanobacteria

The Role of α-Linolenic Acid and Its Oxylipins in Human Cardiovascular Diseases

Contact Info

Product

Resources

About