Specific inhibition of DNA‐polymerases from RNA tumor viruses by some new daunomycin derivatives

Prakash, Chandra; Zunino, Franco; Götz, A.; Gericke, D; Thorbeck, R.; Marco, A. Di

doi:10.1016/0014-5793(72)80179-0

Cited by 60 publications

(13 citation statements)

References 12 publications

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“…Recently, template specific inhibition of DNA polymerase from RNA tumor virus by daunomycin and its derivatives has been reported (CHANDRA et at., 1972). The inhibition exerted by these antibiotics upon the enzyme reaction is selectively dependent on the type of the primer-template used in the assay; it is much more pronounced when poly (dA-dT) or poly rA .…”

Section: Mechanism Of Action: Binding To Dnamentioning

confidence: 98%

Daunomycin (Daunorubicin) and Adriamycin and Structural Analogues: Biological Activity and Mechanism of Action

Marco¹,

Arcamone²,

Zunino³

1975

Mechanism of Action of Antimicrobial and Antitumor Agents

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Section: Mechanism Of Action: Binding To Dnamentioning

confidence: 98%

Daunomycin (Daunorubicin) and Adriamycin and Structural Analogues: Biological Activity and Mechanism of Action

Marco¹,

Arcamone²,

Zunino³

1975

Mechanism of Action of Antimicrobial and Antitumor Agents

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“…Inhibition of this enzyme has been considered to be a highly selective and effective method for interrupting the proliferation of retrovirus (Powell and Darby, 1990). Thus, some inhibitors such as nucleotide analogues [dTTP and dGTP derivatives (Cheng et af., 1987), dUTP derivatives (Tao et af., 1989), AZT (Cox et af., 1991)], antibiotics [daunomycin (Chandra et al 1972), rifamycin (Gurgo et af., 1974), streptonigrin (Oogose et al, 1987)], benzodiazepine derivatives (Pauwels et af., 1990), flavonoids, alkaloids and tannins (Kakiuchi et af., 1985(Kakiuchi et af., , 1987(Kakiuchi et af., , 1991Kusumoto et af., 1991) have been studied hitherto. Some traditional medicines of Japan, China and Sri Lanka (Ayurveda) were also studied in our preliminary screening of those extracts with the aim of finding an inhibitor of RT (Hattori et af., 1991).…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effects of indonesian plant extracts on reverse transcriptase of an RNA Tumour Virus (I)

Kusumoto

Shimada

Kakiuchi

et al. 1992

Phytotherapy Research

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Methanol and water extracts of 30 Indonesian medicinal plants were tested for inhibitory activity on avian myeloblastosis virus (AMV)-reverse transcriptase (RT). The most potent inhibition was shown by extracts of Lorunthus purusiticus (whole plant), Helicteres isoru (fruit), Terminaliu belericu (fruit peel), Strobilanthes crispus (leaves) and Woodfordiu jloribundu (flower and leaves). Two kinds of template-primers, (rA)n-(dT)lz-and (rC)n-(dG)12-18 were examined for polymerization reaction but the inhibitory activity was stronger in the (rC)n-(dG)lz-18 directed reaction in most of the samples. These extracts showed no appreciable cytotoxicity at concentrations where over 90% of RT activity was inhibited.

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“…To date there are several reports dealing with inhibition of viral DNA polymerases (3)(4)(5)(6). The general classification of such inhibitors varies from large synthetic polynucleotides (7) to small molecules such as alkaloids (8) and inorganic cations (9).…”

mentioning

confidence: 99%

Affinity chromatography of viral DNA polymerases on pyran-sepharose.

Chirikjian¹,

Rye²,

Papas³

1975

Proc. Natl. Acad. Sci. U.S.A.

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Pyran covaiently linked to cyanogen bromide-activated Sepharose has been shown to be an effective affinity matrix for several viral DNA polymerases. Differential salt elution of viral compared with cellular polymerases, as well as substrate elution, suggests the affinity nature for the matrix. Unlike some other affinity systems described, pyran-Sepharose is totally resistant to nuclease digestion and is stable at 4°for several months. DNA polymerases isolated from several viruses by detergent treatment were recovered in good yield. Analysis of iodinated proteins by sodium dodecyl sulfate-gel electro-.phoresis revealed that the DNA polymerase of avian myeloblastosis virus found in crude preparations. of the virus could be purified nearly to homogeneity by a single passage through the column. These results suggest that pyran-Sepharose is an effective affinity column that is potentially adaptable as part of a general purification procedure for viral DNA polymerases.

show abstract

Specific inhibition of DNA‐polymerases from RNA tumor viruses by some new daunomycin derivatives

Cited by 60 publications

References 12 publications

Daunomycin (Daunorubicin) and Adriamycin and Structural Analogues: Biological Activity and Mechanism of Action

Daunomycin (Daunorubicin) and Adriamycin and Structural Analogues: Biological Activity and Mechanism of Action

Inhibitory effects of indonesian plant extracts on reverse transcriptase of an RNA Tumour Virus (I)

Affinity chromatography of viral DNA polymerases on pyran-sepharose.

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