Specific inhibitors of auxin transport: Action on tissue segments and in vitro binding to membranes from maize coleoptiles

Depta, H.; Eisele, Karl-Heinz; Hertel, Rainer

doi:10.1016/0304-4211(83)90055-x

Cited by 59 publications

(26 citation statements)

References 22 publications

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“…However, it is rather surprising that NPA and TIBA acted similarly in the sensitivity alteration of the pis1 to different classes. Although these various classes of inhibitors essentially exhibit similar effects, they also (as mutant, since, in addition to the structural difference, NPA and TIBA have been considered to have different binding described above) display a number of differences with regard to both physiological effects and competitive bindsites (Brunn et al, 1992;Depta et al, 1983;Sussman and Goldsmith, 1981;Thomson et al, 1973). An interpretation ing assays.…”

Section: Bap -mentioning

confidence: 99%

“…Although the effects of these classes of inhibitors including growth of seedlings, root elongation, root gravitropism, root phototropism and root curling. The mutant fundamentally overlap with each other, a number of differences are observed both in physiological effects (Gaither also exhibits hypersensitivity to TIBA but the responses to HFCA are unaffected, indicating that the PIS1 gene product and Abeles, 1975;Katekar and Geissler, 1980;Schneider, 1970) and in competitive binding assays (Brunn et al, 1992; might be specifically involved in the response pathway of NPA/TIBA and might act as a negative regulator of the Depta et al, 1983;Sussman and Goldsmith, 1981;Thomson et al, 1973).…”

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confidence: 99%

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PIS1, a negative regulator of the action of auxin transport inhibitors in Arabidopsis thaliana

Fujita

Syōno

1997

The Plant Journal

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In order to clarify the mechanism underlying the polar auxin transport system, the pis1 mutant in Arabidopsis thaliana that is hypersensitive to N‐1‐naphthylphthalamic acid (NPA), an auxin transport inhibitor was isolated and characterized. Whereas the pis1 mutant is normally sensitive to phytohormones, auxins, cytokinin and ethylene precursor, this mutant is hypersensitive to NPA over the broad spectrum of its effects such as growth of seedlings, root elongation, root gravitropism, root phototropism and root curling. This result indicates that the pis1 mutant is specifically affected in the polar auxin transport system. This result also defines a genetic factor controlling both gravitropism and phototropism, and strongly indicates the involvement of auxin transport during both tropic responses. NPA, 2,3,5‐triiodobenzoic acid (TIBA) and 9‐hydroxyfluorene‐9‐carboxylic acid (HFCA) represent different classes of auxin transport inhibitors. The pis1 mutation conferred hypersensitivity to both NPA and TIBA but not to HFCA. These results show the genetic separation of the actions of NPA/TIBA and of HFCA. The PIS1 gene product might be specifically involved in the response pathway of NPA/TIBA, leading to interference with auxin‐efflux carriers, and might act as a negative regulator of the action of NPA/TIBA.

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Section: Bap -mentioning

confidence: 99%

mentioning

confidence: 99%

PIS1, a negative regulator of the action of auxin transport inhibitors in Arabidopsis thaliana

Fujita

Syōno

1997

The Plant Journal

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“…Among them, NPA has been shown to inhibit this transport in both dicotyledonous and monocotyledonous plants by inhibiting active auxin secretion [6]. NPA has also been reported to inhibit polar auxin transport through its binding to a specific site, the so-called NPA receptor in plant cells [7], indicating that the NPA-binding site is important for polar auxin transport [8].…”

Section: Introductionmentioning

confidence: 99%

Differential effects of N-1-naphthylphthalamic acid (NPA) and 2,3,5-triiodobenzoic acid (TIBA) on auxin control of swelling of the shoots of Bryophyllum calycinum Salisb.

et al. 2017

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The effects of N-1-naphthylphthalamic acid (NPA) and 2,3,5-triiodobenzoic acid (TIBA) on the swelling of the stem in intact and decapitated plants of Bryophyllum calycinum in relation to the interaction with auxin, indole-3-acetic acid (IAA), are described. NPA induced conspicuous local internode swelling only in the area of its application in intact plants and in the decapitated internode in the case of simultaneous application of IAA on the top of the internode. By contrast, TIBA applied to an internode of intact plants induced swelling along the entire internode above the treatment area, and similar results were obtained in the decapitated internode when TIBA was applied in the middle of the internode and IAA was applied onto the top of the internode. The differential effect of NPA and TIBA on stem swelling in B. calycinum is discussed in relation to their differential mode of action on auxin transport.

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“…However, the internal structure of these cytokinin-induced pseudonodules differed from that of Rhizobium-induced nodules. Substituted benzoic acids, chemicals that presumably modify auxin levels in the plant (19,20), also induce nodule-like structures on legume roots (refs. 21 resembled Rhizobium-induced nodules at the molecular level.…”

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confidence: 99%

Early nodulin genes are induced in alfalfa root outgrowths elicited by auxin transport inhibitors

Hirsch

Bhuvaneswari

Torrey

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

336

200

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Rhizobium nod genes are essential for root hair deformation and cortical cell division, early stages in the development ofnitrogen-fixing root nodules. Nod-mutants are unable to initiate nodules on legume roots. We observed that N-(1-naphthyl)phthalamic acid and 2,3,5-triiodobenzoic acid, compounds known to function as auxin transport inhibitors, induced nodule-like structures on alfalfa roots. The nodule-like structures (pseudonodules) were white, devoid of bacteria, and resembled nodules elicited by Rhizobium meliloti exopolysaccharide (exo) mutants at both the histological and molecular level. Two nodulin genes, ENOD2 and Nms-30, were expressed. RNA isolated from the nodule-like structures hybridized to pGmENOD2, a soybean early nodulin cDNA clone. RNA isolated from roots did not hybridize. We determined by in vitro translations of total RNA that the alfalfa nodulin transcript Nms-30 was also expressed in the nodule-like structures. The late expressed nodulin genes, such as the leghemoglobin genes, were not transcribed. Because N-(1-naphthyl)-phthalamic acid and 2,3,5-triiodobenzoic acid induce the development of nodules on alfalfa roots, we suggest that the auxin transport inhibitors nmimic the activity of compound(s) made upon the induction of the Rhizobium nod genes.The nodulation (nod) genes of Rhizobium play an essential role in the induction of nodules on the roots of leguminous plants. The (17,18). However, the internal structure of these cytokinin-induced pseudonodules differed from that of Rhizobium-induced nodules. Substituted benzoic acids, chemicals that presumably modify auxin levels in the plant (19,20), also induce nodule-like structures on legume roots (refs. 21 and 22; J.G.T., unpublished results).We found that the histology of the nodule-like structures induced by the auxin transport inhibitors N-(1-naphthyl)-phthalamic acid (NPA) and 2,3,5-triiodobenzoic acid (TIBA)closely resembled that ofRhizobium-induced legume nodules.We studied these nodule-like outgrowths to determine their relation to root nodules initiated by Rhizobium. In

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Specific inhibitors of auxin transport: Action on tissue segments and in vitro binding to membranes from maize coleoptiles

Cited by 59 publications

References 22 publications

PIS1, a negative regulator of the action of auxin transport inhibitors in Arabidopsis thaliana

PIS1, a negative regulator of the action of auxin transport inhibitors in Arabidopsis thaliana

Differential effects of N-1-naphthylphthalamic acid (NPA) and 2,3,5-triiodobenzoic acid (TIBA) on auxin control of swelling of the shoots of Bryophyllum calycinum Salisb.

Early nodulin genes are induced in alfalfa root outgrowths elicited by auxin transport inhibitors

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