Specific Instantaneous Detection of <i>Klebsiella pneumoniae</i> for UTI Diagnosis with a Plasmonic Gold Nanoparticle Conjugated Aptasensor

Deb, Aniruddha; Gogoi, Mousumi; Mandal, Tapas Kumar; Sinha, Swapnil; Pattader, Partho Sarathi Gooh

doi:10.1021/acsabm.3c00369

Cited by 9 publications

(4 citation statements)

References 70 publications

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“…Polyurethane nanoparticles labeled with small molecular probes or fluorophores can be used as different imaging agents for magnetic resonance imaging (Figure ) and other imaging techniques . With nanoimaging, the wounded or diseased area can be targeted with greater precision than with conventional contrast agents, which makes imaging easier. − …”

Section: Biomedical Applicationsmentioning

confidence: 99%

Polyurethane Nanoparticles as Versatile Tools in Nanomedicine: A Review

Saha,

Trivedi,

Rengan

et al. 2024

ACS Appl. Nano Mater.

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The rapidly advancing field of nanomedicine has witnessed the emergence of polyurethane nanoparticles (PUNPs) as exceptionally versatile tools with transformative potential. This comprehensive review dives into the various roles that PUNPs play in the realm of nanomedicine, positioning them as pivotal components in therapeutic, diagnostic, and theranostic applications. With a focus on their unique characteristics, these nanoparticles offer a platform for precision at the nanoscale, a critical attribute in the intricate landscape of medical interventions. This review precisely navigates through the biobased synthesis method and the recent applications (2019−2023) of PUNPs in the therapeutic, diagnostic, and theranostic fields. Furthermore, it explores innovative approaches in surface functionalization, elucidating how tailored modifications can optimize their interaction with biological entities. Beyond the conventional realm, PUNPs emerge as carriers of therapeutic cargo and strategic players in diagnostics and theranostics, showcasing their multifaceted utility. In conclusion, this review navigates beyond generalized statements, offering a tailored exploration into the specific attributes that make polyurethane nanoparticles indispensable in advancing the frontiers of nanomedicine. Through a meticulous examination of their synthesis, functionalization, and applications, this review aims to contribute a different understanding of PUNPs' versatile roles in drug delivery systems, imaging, and theranostics.

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Section: Biomedical Applicationsmentioning

confidence: 99%

Polyurethane Nanoparticles as Versatile Tools in Nanomedicine: A Review

Saha,

Trivedi,

Rengan

et al. 2024

ACS Appl. Nano Mater.

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“…Deb et al [37] described a genetic approach for the fabrication of a prototype to the noninvasive detection of K. pneumoniae MTCC-7028 using a tailor-made plasmonic aptamergold nanoparticle (AuNP)assay. The sequence of the aptamer KPBA1 was 5 ′ -/5ThioMC6-D/GGCTGGATGGGGCGTGTGGAGCCCCGTTAGAATATCAGAGGTGGTGGCAACG-GTGCGGACAGCG-3 ′ .…”

Section: Selection and Characterization Of Dna Aptamers To Detect K P...mentioning

confidence: 99%

Aptamers: A Cutting-Edge Approach for Gram-Negative Bacterial Pathogen Identification

Córdova-Espinoza,

González-Vázquez,

Barron-Fattel

et al. 2024

IJMS

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Early and accurate diagnoses of pathogenic microorganisms is essential to correctly identify diseases, treating infections, and tracking disease outbreaks associated with microbial infections, to develop precautionary measures that allow a fast and effective response in epidemics and pandemics, thus improving public health. Aptamers are a class of synthetic nucleic acid molecules with the potential to be used for medical purposes, since they can be directed towards any target molecule. Currently, the use of aptamers has increased because they are a useful tool in the detection of specific targets. We present a brief review of the use of aptamers to detect and identify bacteria or even some toxins with clinical importance. This work describes the advances in the technology of aptamers, with the purpose of providing knowledge to develop new aptamers for diagnoses and treatment of different diseases caused by infectious microorganisms.

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“…Another alternative approach for biorecognition focuses on the use of DNA aptamers, which are characterized by high sensitivity and selectivity, and a lower cost than expensive antibodies. , In this case, binding to the target generally occurs as a function of the specific 3D shape of the aptamer. Many aptamers have been developed to bind whole bacterial cells, , including those of S. aureus and B.…”

Section: Introductionmentioning

confidence: 99%

“… 15 , 16 In this case, binding to the target generally occurs as a function of the specific 3D shape of the aptamer. Many aptamers have been developed to bind whole bacterial cells, 39 , 40 including those of S. aureus and B. cereus . 41 , 42…”

Section: Introductionmentioning

confidence: 99%

Rapid Detection and Identification of Vancomycin-Sensitive Bacteria Using an Electrochemical Apta-Sensor

Novakovic,

Khalife,

Costache

et al. 2024

ACS Omega

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In order to combat the complex and diverse infections caused by bacteria, it is essential to develop efficient diagnostic tools. Current techniques for bacterial detection rely on laborious multistep procedures, with high costs and extended time of analysis. To overcome these limitations, we propose here a novel portable electrochemical biosensor for the rapid detection and identification of Gram-positive bacteria that leverages the recognition capabilities of vancomycin and aptamers. A vancomycin-modified screen-printed carbon electrode was used to selectively capture Gram-positive bacteria susceptible to this antibiotic. Electrochemical impedance spectroscopy and scanning electron microscopy demonstrated that capture was achieved in 10 min, with a limit of detection of only 2 CFU/mL. We then tested the device's potential for aptamer-based bacterial identification using Staphylococcus aureus and Bacillus cereus as the test strains. Specifically, electrodes with captured bacteria were exposed to species-specific aptamers, and the resulting changes in current intensity were analyzed using differential pulse voltammetry. When used directly in untreated milk or serum, the system was able to successfully identify a small amount of S. aureus and B. cereus (100 CFU/mL) in less than 45 min. This novel biosensor has the potential to serve as an invaluable tool that could be used, even by inexperienced staff, in a broad range of settings including clinical diagnostics, food safety analysis, environmental monitoring, and security applications.

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Specific Instantaneous Detection of Klebsiella pneumoniae for UTI Diagnosis with a Plasmonic Gold Nanoparticle Conjugated Aptasensor

Cited by 9 publications

References 70 publications

Polyurethane Nanoparticles as Versatile Tools in Nanomedicine: A Review

Polyurethane Nanoparticles as Versatile Tools in Nanomedicine: A Review

Aptamers: A Cutting-Edge Approach for Gram-Negative Bacterial Pathogen Identification

Rapid Detection and Identification of Vancomycin-Sensitive Bacteria Using an Electrochemical Apta-Sensor

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