Specific interaction between HPV-16 E1–E4 and cytokeratins results in collapse of the epithelial cell intermediate filament network

Doorbar, John; Ely, Sarah; Sterling, Jane; McLean, C. S.; Crawford, L. V.

doi:10.1038/352824a0

Cited by 289 publications

(202 citation statements)

References 21 publications

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“…Although its ORF resides in the early region of the genome, it is expressed in the late stage of the virus life-cycle. The E4 protein of HPV-16 was reported to induce the collapse of the cytokeratin matrix of infected keratinocytes (Doorbar et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

Functional significance of sequence variation in the E2 gene and the long control region of human papillomavirus type 16.

Veress

Szarka

Dong³

et al. 1999

Journal of General Virology

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The long control region (LCR) and the E2 protein of human papillomaviruses (HPV) are the most important viral factors regulating transcription of the viral oncogenes E6 and E7. Sequence variation within these genomic regions may have an impact on the oncogenic potential of the virus. Sequence variation in the LCR and in the E2 gene of human papillomavirus type 16 (HPV-16) isolates originating from cervical cancer patients from East Hungary was studied. In 30 samples, sequencing and/or single-strand conformation polymorphism analysis revealed variants belonging to the European variant lineage of HPV-16. These variants differed from the reference European clone only slightly in their E2 and LCR sequences. Three samples represented variants belonging to the Asian-American group. These differed from the published reference sequence at several positions in the LCR and E2 regions. Compared to the reference clone, the LCR clones of the European isolates showed very similar transcriptional activities, while that of an Asian-American isolate had " 1n7-fold increased activity. Most of the increased activity of the Asian-American isolate could be ascribed to nucleotide changes found at the 3h end of the LCR (nt 7660-7890). The transcriptional transactivation potentials of the HPV-16 E2 isolates differed only slightly from each other, and the differences seemed to be independent of the taxonomic position of the isolates.

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Section: Discussionmentioning

confidence: 99%

Functional significance of sequence variation in the E2 gene and the long control region of human papillomavirus type 16.

Veress

Szarka

Dong³

et al. 1999

Journal of General Virology

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“…It is exclusively localized within the differentiating layer of the infected epithelium and induces the collapse of the cytokeratin network (Doorbar et al 1991;zur Hausen 1996).…”

Section: E4 Proteinmentioning

confidence: 99%

Human papillomavirus type 16 in head and neck carcinogenesis

2005

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“…The E4 protein causes dramatic changes in the cytokeratin network altering the cytoplasmic environment of spinous cells, which contributes to the HPV induced cytopathic effect and koilocytosis [12][13][14]. These changes accelerate the release of virions assembled due to self-aggregation of the L1/L2 protein complexes.…”

Section: Skin Infectionmentioning

confidence: 99%

Diagnostic role of p16/INK4A protein in Human Papillomavirus (HPV) induced cervical dysplasia

Rajčáni¹,

Adamkov²,

et al. 2010

Open Life Sciences

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Abstract:The p16/INK4A protein is a cellular regulatory polypeptide over-expressed in the presence of high levels of the Human Papillomavirus (HPV) coded E7 protein. This review outlines the use of p16 antigen staining in cervical biopsies as well as in PAP smears summarizing the corresponding literature and commenting the authors' own experience. The p16 antigen is a reliable marker for dysplastic cells in CINII/CINIII (HSIL) lesions as viewed in cervical biopsies. When PAP smears were examined at large scale screening for p16 antigenreactive and atypical cells, considerable variations could be found especially in ASCUS graded lesions. Therefore, the presence of p16-reactive atypical cells in PAP smears should be interpreted together with the cytological signs of dysplasia, such as the altered N/C ratio. In addition, women revealing p16-positive ASCUS/LSIL specimens should be examined for the presence of HPV DNA. Detection of HPV DNA alone, i.e. in the absence of cytological screening has a low predictive value, since the clearance of HPV may occur even in the absence of morphological alterations. Combined cytological as well as molecular follow up contributes to the efficiency of diagnostic and increases the probability of correct interpretation of the pre-cancerous lesions by non-invasive techniques.

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Specific interaction between HPV-16 E1–E4 and cytokeratins results in collapse of the epithelial cell intermediate filament network

Cited by 289 publications

References 21 publications

Functional significance of sequence variation in the E2 gene and the long control region of human papillomavirus type 16.

Functional significance of sequence variation in the E2 gene and the long control region of human papillomavirus type 16.

Human papillomavirus type 16 in head and neck carcinogenesis

Diagnostic role of p16/INK4A protein in Human Papillomavirus (HPV) induced cervical dysplasia

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