Specific Interaction of Escherichia coli 0157:H7-Derived Shiga-like Toxin II with Human Renal Endothelial Cells

Louise, Chandra B.; Obrig, Tom G.

doi:10.1093/infdis/172.5.1397

Cited by 197 publications

(136 citation statements)

References 13 publications

Supporting

Mentioning

126

Contrasting

Unclassified

Order By: Relevance

“…Surface plasmon resonance analysis showed that Stx-1 easily binds to and detaches from Gb3, in contrast to Stx-2, which binds slowly but also dissociates very slowly, thus staying on the cells long enough to be incorporated (62). The latter could explain why Stx-2 is 1000-fold more toxic than Stx-1 on human endothelial cells in vitro (63).…”

Section: Shiga Toxin or Shiga Toxins?mentioning

confidence: 99%

Hemolytic Uremic Syndrome

Noris¹,

Remuzzi²

2005

Journal of the American Society of Nephrology

502

428

View full text Add to dashboard Cite

Section: Shiga Toxin or Shiga Toxins?mentioning

confidence: 99%

Hemolytic Uremic Syndrome

Noris¹,

Remuzzi²

2005

Journal of the American Society of Nephrology

502

428

View full text Add to dashboard Cite

“…Epidemiological studies, together with in vivo and in vitro experiments, have revealed that stx2 (and its variants) is the most important virulence factor associated with severe human disease. STEC producing stx2 is more commonly associated with serious disease than isolates producing stx1 or stx1 plus stx2 (Boerlin et al, 1999;Louise & Obrig, 1995;Paton & Paton, 1998). In the current study, the majority of strains were stx1-producing strains (24/29, 83 %); only 5/29 (17 %) strains carried stx2.…”

Section: Distribution Of Virulence Genesmentioning

confidence: 99%

Phylogenetic relationships of Shiga toxin-producing Escherichia coli isolated from Peruvian children

Contreras

Ochoa

Ruiz

et al. 2011

Journal of Medical Microbiology

View full text Add to dashboard Cite

The aim of this study was to determine the prevalence, virulence factors (stx, eae, ehxA and astA) and phylogenetic relationships [PFGE and multilocus sequence typing (MLST)] of Shiga toxinproducing Escherichia coli (STEC) strains isolated from four previous cohort studies in 2212 Peruvian children aged ,36 months. STEC prevalence was 0.4 % (14/3219) in diarrhoeal and 0.6 % (15/2695) in control samples. None of the infected children developed haemolytic uraemic syndrome (HUS) or other complications of STEC. stx1 was present in 83 % of strains, stx2 in 17 %, eae in 72 %, ehxA in 59 % and astA in 14 %. The most common serotype was O26 : H11 (14 %) and the most common seropathotype was B (45 %). The strains belonged mainly to phylogenetic group B1 (52 %). The distinct combinations of alleles across the seven MLST loci were used to define 13 sequence types among 19 STEC strains. PFGE typing of 20 STEC strains resulted in 19 pulsed-field patterns. Comparison of the patterns revealed 11 clusters (I-XI), each usually including strains belonging to different serotypes; one exception was cluster VI, which gathered exclusively seven strains of seropathotype B, clonal group enterohaemorrhagic E. coli (EHEC) 2 and phylogenetic group B1. In summary, STEC prevalence was low in Peruvian children with diarrhoea in the community setting. The strains were phylogenetically diverse and associated with mild infections. However, additional studies are needed in children with bloody diarrhoea and HUS.

show abstract

“…Epidemiological studies, together with in vivo and in vitro experiments, have revealed that Stx2 is the most important virulence factor associated with severe human disease. Indeed, Stx2 is 1000 times more cytotoxic than Stx1 towards human renal endothelial cells, and STEC producing Stx2 are more commonly associated with serious diseases than isolates producing Stx1 or Stx1 plus Stx2 (Louise & Obrig, 1995;Boerlin et al, 1999;Paton & Paton, 1998). Several Stx2 variants have been identified on the basis of sequence homology and immunological crossreactivity (Ito et al, 1990;Schmitt et al, 1991;Paton et al, 1995;Friedrich et al, 2002;Pierard et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Differential expression of stx2 variants in Shiga toxin-producing Escherichia coli belonging to seropathotypes A and C

Sablet¹,

Bertin²,

Vareille³

et al. 2008

Microbiology

View full text Add to dashboard Cite

Only a subset of Shiga toxin (Stx)-producing Escherichia coli (STEC) are human pathogens, but the characteristics that account for differences in pathogenicity are not well understood. In this study, we investigated the distribution of the stx variants coding for Stx2 and its variants in highly virulent STEC of seropathotype A and low-pathogenic STEC of seropathotype C. We analysed and compared transcription of the corresponding genes, production of Shiga toxins, and stx-phage release in basal as well as in induced conditions. We found that the stx 2 variant was mainly associated with strains of seropathotype A, whereas most of the strains of seropathotype C possessed the stx 2-vhb variant, which was frequently associated with stx 2 , stx 2-vha or stx 2c . Levels of stx 2 and stx 2 -related mRNA were higher in strains belonging to seropathotype A and in those strains of seropathotype C that express the stx 2 variant than in the remaining strains of seropathotype C. The stx 2-vhb genes were the least expressed, in basal as well as in induced conditions, and in many cases did not seem to be carried by an inducible prophage. A clear correlation was observed between stx mRNA levels and stx-phage DNA in the culture supernatants, suggesting that most stx 2 -related genes are expressed only when they are carried by a phage. In conclusion, some relationship between stx 2 -related gene expression in vitro and the seropathotype of the STEC strains was observed. A higher expression of the stx 2 gene and a higher release of its product, in basal as well as in induced conditions, was observed in pathogenic strains of seropathotype A. A subset of strains of seropathotype C shows the same characteristics and could be a high risk to human health.

show abstract

Specific Interaction of Escherichia coli 0157:H7-Derived Shiga-like Toxin II with Human Renal Endothelial Cells

Cited by 197 publications

References 13 publications

Hemolytic Uremic Syndrome

Hemolytic Uremic Syndrome

Phylogenetic relationships of Shiga toxin-producing Escherichia coli isolated from Peruvian children

Differential expression of stx2 variants in Shiga toxin-producing Escherichia coli belonging to seropathotypes A and C

Contact Info

Product

Resources

About