Specific Interferon γ Detection for the Diagnosis of Previous Q Fever

Schoffelen, Teske; Joosten, Leo A. B.; Herremans, Tineke; Haan, A.F.J. de; Ammerdorffer, Anne; Rümke, H. C.; Wijkmans, C.J.; Roest, H.I.J.; Netea, Mihai G.; Meer, J.W.M. van der; Sprong, Tom; Deuren, Marcel van

doi:10.1093/cid/cit129

Cited by 36 publications

(55 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previously, our group showed that antigen-specific IFNγ production was significantly higher in QFS patients ( n = 28; 319.4 pg/mL) than in Q fever seropositive controls ( n = 135; 120 pg/mL) [8]. It was also shown that antigen-specific IFNγ production is a useful tool for diagnosing a previous Q fever infection [13], and adding antigen-specific IL-2 production helped discriminate chronic Q fever patients from Q fever seropositive controls and QFS patients [8, 17]. In this study, antigen-specific IFNγ production was not significantly higher in QFS patients than in asymptomatic Q fever seropositive controls.…”

Section: Discussionmentioning

confidence: 99%

Interferon-γ and CXCL10 responses related to complaints in patients with Q fever fatigue syndrome

Raijmakers

Jansen

Keijmel

et al. 2018

Eur J Clin Microbiol Infect Dis

Self Cite

View full text Add to dashboard Cite

Approximately 20% of patients with acute Q fever develop Q fever fatigue syndrome (QFS), a debilitating fatigue syndrome. This study further investigates the role of C. burnetii-specific IFNγ, but also IL-2, CXCL9, CXCL10, and CXLC11 production in QFS patients. C. burnetii-specific IFNy, IL-2, CXCL9, CXCL10, and CXCL11 production were tested in ex vivo stimulated whole blood of QFS patients who recovered from their complaints (n = 8), QFS patients with persisting complaints (n = 27), and asymptomatic Q fever seropositive controls (n = 10). With the exclusion of one outlier, stimulation with C. burnetii revealed significantly higher IFNy and CXCL10 production in QFS patients with persisting complaints (medians 288.0 and 176.0 pg/mL, respectively) than in QFS patients who recovered from their complaints (medians 93.0 and 85.5 pg/mL, respectively) (p = 0.041 and 0.045, respectively). No significant differences between groups were found for C. burnetii-specific IL-2, CXCL9, and CXCL11 production. These findings point towards a difference in cell-mediated immunity in QFS patients with persisting complaints compared to those who recovered from their complaints. Such a difference may aid to eventually diagnose QFS more objectively and might serve as an indicator of its underlying etiology.

show abstract

Section: Discussionmentioning

confidence: 99%

Interferon-γ and CXCL10 responses related to complaints in patients with Q fever fatigue syndrome

Raijmakers

Jansen

Keijmel

et al. 2018

Eur J Clin Microbiol Infect Dis

Self Cite

View full text Add to dashboard Cite

show abstract

“…Antibodies against C. burnetii (IgG against phase 1 and phase 2) were measured in 1:32 serum dilution, as described [21]. If positive, exact IgG and IgM titres were determined.…”

Section: Serologymentioning

confidence: 99%

“…In vitro IFN-␥ production after C. burnetii stimulation, measured in a whole-blood assay [21], was expressed as continuous variable with cut-off at 32 pg/mL, based on previous findings [21]. Outcome did not affect the decision to vaccinate.…”

Section: Burnetii Ifn-production-assaymentioning

confidence: 99%

“…This may have introduced a bias, with underestimation of found associations. We previously published limited immune responses in this old-age group with much co-morbidity, with as many as 23% negative serology and IFN-␥ values after six months [21]. However, despite limited serological responses to vaccination, Australian studies have shown high protection in occupationally exposed people [30,[42][43][44].…”

Section: Skin-testmentioning

confidence: 99%

“…AE following skin test and vaccination were monitored intensively, with follow-up for 90 days. An investigational whole-blood interferon-gamma (IFN-␥) production assay, measuring cell-mediated immunity in vitro, was added to the screening [21,22]. While its outcome was irrelevant for the decision to vaccinate, it gave an opportunity to analyze AE in vaccinees with possibly pre-existing cell-mediated immunity.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Adverse events and association with age, sex and immunological parameters of Q fever vaccination in patients at risk for chronic Q fever in the Netherlands 2011

Schoffelen

Wong

Rümke³

et al. 2014

Vaccine

Self Cite

View full text Add to dashboard Cite

Intact interferon-γ response against Coxiella burnetii by peripheral blood mononuclear cells in chronic Q fever

Schoffelen

Textoris

Bleeker‐Rovers

et al. 2017

Clinical Microbiology and Infection

View full text Add to dashboard Cite

show abstract

Specific Interferon γ Detection for the Diagnosis of Previous Q Fever

Abstract: Specific IFN-γ detection is a novel diagnostic assay for previous C. burnetii infection and shows similar performance and practical advantages over serology and ST. Future studies to investigate the clinical value in practice are warranted.

Cited by 36 publications

References 27 publications

Interferon-γ and CXCL10 responses related to complaints in patients with Q fever fatigue syndrome

Interferon-γ and CXCL10 responses related to complaints in patients with Q fever fatigue syndrome

Adverse events and association with age, sex and immunological parameters of Q fever vaccination in patients at risk for chronic Q fever in the Netherlands 2011

Intact interferon-γ response against Coxiella burnetii by peripheral blood mononuclear cells in chronic Q fever

Contact Info

Product

Resources

About