Specific Lymphocyte Sensitization in Chronic Pancreatitis

Schütt, Christine; Friemel, H; Schulze, Hans-Arthur; Zubaidi, G

doi:10.1159/000197723

Cited by 12 publications

(8 citation statements)

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“…Murray and Thal [1] and Fonkalsrud and Longemire [2] have found autoantibodies in patients with chronic pancreatitis. Schütt et al [3] demonstrated autoreactive peripheral blood lymphocytes. Bedossa et al [4], Jalleh et al 1 Leukocytes in Pancreatic Tissue Digestion 1998;59:192-198 193 [5], and our group [6] detected aberrant HLA-DR expression in pancreatic tissue of chronic pancreatitis patients.…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical Characterization of the Pancreatic Cellular Infiltrate in Normal Pancreas, Chronic Pancreatitis and Pancreatic Carcinoma

Emmrich¹,

Weber²,

Nausch³

et al. 1998

Digestion

101

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Background/Aims: Chronic pancreatitis is histologically characterized by an extended fibrosis and infiltration of leukocytes. We intended to differentiate the infiltration to evaluate the inflammatory process. Methods: Samples of tissues of normal pancreas (NP, n = 12), of chronic pancreatitis (CP, n = 7), and pancreatic tissues surrounding pancreatic carcinoma (CA, n = 7) were investigated by immunohistochemical staining using the APAAP technique. Results: In normal pancreas, mononuclear cells (47.1 ± 26.0 cells/mm²) were observed with a predominance of macrophages (56.3%) and T lymphocytes (31.3%) which were differentiated in CD8+ lymphocytes (9.3 ± 7.2 cells/mm²) and CD4+ lymphocytes (6.7 ± 3.2 cells/mm²). Rarely, plasma cells (5.3%) and B lymphocytes (7.1%) could be detected. In pancreatic tissue of patients with CP and in CA there was a significant increase of mononuclear cells to 264.4 ± 120.3 cells/mm² and 284.3 ± 67.8 cells/mm², respectively. In both diseases percentages of T lymphocytes (CP: 50.5%; CA: 48.1%) were higher than in normal controls. CD4+/CD8+ ratio of 0.77 in CP and 0.82 in CA demonstrated a predominance of CD8+ cells compared to the peripheral blood. In NP and CA, nearly all T lymphocytes expressed CD45R0 identifying memory cells, while only 58% of T lymphocytes were CD45R0 positive in CP. Conclusion: Our data suggest that the investigated cases of CP were of a common inflammatory type rather than due to an autoimmunological reaction. CD8+ T lymphocytes were the predominant T cell subset in the inflammatory infiltrates in both CP and CA.

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Section: Introductionmentioning

confidence: 99%

Immunohistochemical Characterization of the Pancreatic Cellular Infiltrate in Normal Pancreas, Chronic Pancreatitis and Pancreatic Carcinoma

Emmrich¹,

Weber²,

Nausch³

et al. 1998

Digestion

101

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“…Au toimmune mechanisms may contribute to the pathogene sis of chronic pancreatitis [16][17][18][19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Expression of Transforming Growth Factor-β<sub>1</sub> in Chronic Pancreatitis

Slater¹,

Williamson

Foster

1995

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Transforming growth factor-β₁ (TGF-β₁) is a multifunctional cytokine which modifies tissue stromal matrix synthesis, cell proliferation and immune function. In the present study, we have used a mouse monoclonal antibody to the latent (intracytoplasmic) form of TGF-β₁ to compare its expression in 144 cases of human chronic pancreatitis (both obstructive and chronic calcifying) with that of 10 control pancreatic specimens. In all the control specimens, and most of those with chronic pancreatitis, cytoplasmic immunoreactivity was identified in pancreatic duct and ductular epithelium, in islet cells and in vascular smooth muscle and endothelium. Two distinct patterns of ductular epithelial staining emerged: in morphologically normal tissues, only individual distal ductular/centroacinar cells stained but in chronic pancreatitis (whether chronic calcifying or chronic obstructive) the staining was panductular. Positive cytoplasmic immunostaining of acinar epithelial cells was found in 3% of pancreatitis specimens but in none of the normal controls. There was no staining of fibroblasts. Synthesis of TGF-β₁ appears to be normally restricted to a population of epithelial cells located in terminal/centroacinar regions of the ductules (together with occasional ductal cells) whereas in chronic pancreatitis, TGF-β₁ is expressed in most ductular and ductal epithelial cells.

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“…It was also reported that lymphocytes were sensitized specifically with the organ-specific human pancreatic antigens in most cases of chronic pancreatitis in man (16,17,21), and that antinuclear factors were detected in the serum of most patients with acute and chronic pancreatitis of unknown etiology (13). These results suggest that immunological mechanisms are involved in the development of some types of chronic pancreatitis in man.…”

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confidence: 99%

Microbial Adjuvant and Autoimmunity

Yamaki

Ohta

Nakashima

et al. 1980

Microbiology and Immunology

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Definite lesions in the exocrine pancreas were produced when SMA mice were immunized eight times at intervals of 30 days with a mixture of extract of pooled pancreas from syngeneic mice and the capsular polysaccharide of Klebsiella pneumoniae type 1 Kasuya strain (CPS-K), whereas no pancreatic lesions were produced in mice given CPS-K alone or pancreatic extract alone. The typical histological changes were characterized by infiltration with lymphocytes, plasma cells, and other mononuclear cells, degeneration and lysis of the acinar cells, destruction of the lobular architecture, and replacement by fatty tissue and fibrous connective tissue. The endocrine islets were well preserved. No specific histological changes were produced in the organs other than the pancreas in these mice. Most of mice immunized with pancreatic extract mixed with CPS-K produced serum precipitins to syngeneic pancreatic antigens. However, severe pancreatic lesions were also produced in mice showing no definite precipitin production.Occurrence of circulating antibodies to the pancreas has been described in chronic pancreatitis and other pancreatic diseases (3,4,(18)(19)(20)(21). It was also reported that lymphocytes were sensitized specifically with the organ-specific human pancreatic antigens in most cases of chronic pancreatitis in man (16,17,21), and that antinuclear factors were detected in the serum of most patients with acute and chronic pancreatitis of unknown etiology (13). These results suggest that immunological mechanisms are involved in the development of some types of chronic pancreatitis in man. It was reported that acute interstitial pancreatitis and elevation in the serum amylase values were induced after injection of rabbit anti-guinea pig pancreas antiserum into guinea pigs (14). Rabbit anti-dog pancreas antiserum was also found to induce serum amylase increases within 4 days when administered passively to dogs, although the histological appearance of the 945

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Specific Lymphocyte Sensitization in Chronic Pancreatitis

Abstract: Specific lymphocyte sensitization was found in vitro using a lymphocyte transformation test with human pancreatic antigens. It should be possible to develop an immunological diagnostic test for chronic pancreatitis.

Cited by 12 publications

References 1 publication

Immunohistochemical Characterization of the Pancreatic Cellular Infiltrate in Normal Pancreas, Chronic Pancreatitis and Pancreatic Carcinoma

Immunohistochemical Characterization of the Pancreatic Cellular Infiltrate in Normal Pancreas, Chronic Pancreatitis and Pancreatic Carcinoma

Expression of Transforming Growth Factor-β<sub>1</sub> in Chronic Pancreatitis

Microbial Adjuvant and Autoimmunity

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