Specific non-saturable binding of insulin by human platelets

Setiadi, Hendra; Herington, Adrian C.

doi:10.1016/0303-7207(85)90038-3

Cited by 7 publications

(3 citation statements)

References 30 publications

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“…35,36 However, there are no data on the similar effect of these molecules on the vertebrate insulin receptor, but there are data on the ineffectiveness of these hormones on binding of insulin in mammalian model systems. [37][38][39] Also interesting is the behaviour of binding after histamine and EGF treatment (Figure 3). Both hormones allow binding at control level up to 15 min, but subsequent histamine treatment causes a significant decrease.…”

Section: Resultsmentioning

confidence: 99%

Effects of extremely low concentrations of hormones on the insulin binding of Tetrahymena

Csaba

Kovács

Tóthfalusi

et al. 2006

Cell Biology International

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FITC-insulin binding to previously hormone-treated Tetrahymena was studied by flow cytometry and confocal microscopy. Hormones produced by Tetrahymena were chosen for study and the hormone concentrations were administered between 10(-6) and 10(-21)M for 30 min. Endorphin, serotonin and insulin significantly reduced the hormone binding however histamine did not influence it at all. Endorphin, serotonin and insulin were significantly effective down to 10(-18)M and the effect of insulin and endorphin suggest a similar mechanism. The results call attention to the efficacy of very low hormone concentrations, which can influence the hormone content (earlier experiments) and receptor binding capacity (present study) of a unicellular organism. This seems to be very important, as in wild (natural) conditions the dilution of signaling materials secreted by a water-living protozoan is very high. In addition, the results point to the selectivity of response, as not all of the hormones that deeply influence other physiological indices (e.g. histamine) have an effect on insulin content or insulin receptors.

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Section: Resultsmentioning

confidence: 99%

Effects of extremely low concentrations of hormones on the insulin binding of Tetrahymena

Csaba

Kovács

Tóthfalusi

et al. 2006

Cell Biology International

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“…The discrepancy between these results and ours may be related to different experimental conditions, since these authors used unwashed platelet pellets. It is then not possible to exclude a contamination by other growth factors such as insulin [21] or IGF I and I1 [22] which are known to bind to platelets [23].…”

Section: Discussionmentioning

confidence: 99%

Platelet‐derived growth factor (PDGF) in type 1 diabetes mellitus

Guillausseau¹,

Dupuy

Bryckaert

et al. 1989

Eur J Clin Investigation

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In 10 patients with type 1 diabetes mellitus, platelet-derived growth factor (PDGF) was assessed using an in vitro assay based on the stimulation of DNA synthesis in the 3T3 mouse fibroblast cell line. beta-Thromboglobulin (beta-TG) was used as an index for platelet alpha-granules content. Platelet beta-TG content and PDGF were markedly decreased in diabetic patients while plasma beta-TG was increased as compared with control subjects. In diabetic patients, a significant negative correlation was found between plasma beta-TG level and beta-TG total platelet content, associated with a significant positive correlation between platelet beta-TG content and PDGF. These results suggest that PDGF release might be increased in diabetic subjects. This may account in part for the cell proliferation observed in diabetic angiopathy.

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“…93 In other studies, 50 µM colchicine had no effect on the endocytosis of luciferase yellow carbohydrazide in pig platelets. 94 High concentrations of colchicine inhibit platelet serotonin uptake 95 and insulin binding, 96 and also block the concanavalin A-mediated inhibition of platelet serotonin uptake. 97 Nanomolar concentrations of colchicine increase the binding of vinblastine to platelets, suggesting a potential synergistic interaction between these two agents.…”

Section: Colchicine and Platelet-mediated Binding Endocytosis And Pro...mentioning

confidence: 99%

Colchicine as a Modulator of Platelet Function: A Systematic Review

Reddel

Pennings

Chen

et al. 2022

Semin Thromb Hemost

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The microtubule inhibitor and anti-inflammatory agent colchicine is used to treat a range of conditions involving inflammasome activation in monocytes and neutrophils, and is now known to prevent coronary and cerebrovascular events. In vitro studies dating back more than 50 years showed a direct effect of colchicine on platelets, but as little contemporary attention has been paid to this area, we have critically reviewed the effects of colchicine on diverse aspects of platelet biology in vitro and in vivo. In this systematic review we searched Embase, Medline, and PubMed for articles testing platelets after incubation with colchicine and/or reporting a clinical effect of colchicine treatment on platelet function, including only papers available in English and excluding reviews and conference abstracts. We identified 98 relevant articles and grouped their findings based on the type of study and platelet function test. In vitro, colchicine inhibits traditional platelet functions, including aggregation, clotting, degranulation, and platelet-derived extracellular vesicle formation, although many of these effects were reported at apparently supraphysiological concentrations. Physiological concentrations of colchicine inhibit collagen- and calcium ionophore-induced platelet aggregation and internal signaling. There have been limited studies of in vivo effects on platelets. The colchicine-platelet interaction has the potential to contribute to colchicine-mediated reduction in cardiovascular events, but there is a pressing need for high quality clinical research in this area.

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Specific non-saturable binding of insulin by human platelets

Cited by 7 publications

References 30 publications

Effects of extremely low concentrations of hormones on the insulin binding of Tetrahymena

Effects of extremely low concentrations of hormones on the insulin binding of Tetrahymena

Platelet‐derived growth factor (PDGF) in type 1 diabetes mellitus

Colchicine as a Modulator of Platelet Function: A Systematic Review

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