Specific Nucleotide Changes in the Subgenomic Promoter Region Influence Infectivity of the Sindbis Virus

Zhu, Wuyang; Zhao, Ting; Liang, Guodong

doi:10.1159/000351081

Cited by 1 publication

(1 citation statement)

References 19 publications

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“…Since conserved sequence elements (CSEs) have important roles in the lifecycle of alphaviruses (Fayzulin and Frolov, 2004;Hyde et al, 2015;Niesters and Strauss, 1990;Zhu et al, 2013), we investigated the sequence identities of CSEs with other defined alphaviruses. The CSEs of MWAV within the nsP1 51 nt, subgenomic promoter sequence and the 3'-UTR shared more than 90% nt sequence identities with EILV and TALV (Fig.…”

Section: Main Textmentioning

confidence: 99%

Discovery of Mwinilunga alphavirus: A novel alphavirus in Culex mosquitoes in Zambia

et al. 2018

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Mosquito-borne alphaviruses are disseminated globally and cause febrile illness in humans and animals. Since the prevalence and diversity of alphaviruses has not been previously investigated in Zambia, reverse transcription PCR was employed as a broad-spectrum approach for the detection of alphaviruses in mosquitoes. From 552 mosquito pools, a novel alphavirus, tentatively named Mwinilunga alphavirus (MWAV), was discovered from a single Culex quinquefasciatus mosquito pool. The full genome of MWAV was subsequently determined, and pairwise comparisons demonstrated that MWAV represented a new alphavirus species. Phylogenetic analyses and a linear discriminant analysis based on the dinucleotide ratios in various virus sequences indicated that MWAV is related to a mosquito-specific alphavirus distinct from other known mosquito-borne alphaviruses due to its inability to replicate in vertebrate cell lines. Further analyses of these novel alphaviruses will help to facilitate a greater understanding of the molecular determinants of host range restriction and the evolutionary relationships of alphaviruses.

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Section: Main Textmentioning

confidence: 99%