Specific Oxidative Cleavage of Carotenoids by VP14 of Maize

Schwartz, Steven H.; Tan, Bao Cai; Gage, Douglas A.; Zeevaart, Jan A. D.; McCarty, Donald R.

doi:10.1126/science.276.5320.1872

Cited by 817 publications

(580 citation statements)

References 18 publications

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“…To determine the cause of the flooding-induced drop in ABA concentration, expression of ABA metabolism genes was studied. ABA accumulation is commonly controlled at the level of synthesis, at the step catalyzed by 9-cis-epoxycarotenoid dioxygenase (NCED)-type enzymes (Schwartz et al, 1997). Both of the identified ABA synthesis-related NCED orthologs were strongly down-regulated 24 h after the start of flooding or ethylene treatment ( Fig.…”

Section: Reduction Of Aba Levels Acts Downstream Of Ethylene In the Amentioning

confidence: 99%

A Co-Opted Hormonal Cascade Activates Dormant Adventitious Root Primordia upon Flooding in Solanum dulcamara

et al. 2016

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Section: Reduction Of Aba Levels Acts Downstream Of Ethylene In the Amentioning

confidence: 99%

A Co-Opted Hormonal Cascade Activates Dormant Adventitious Root Primordia upon Flooding in Solanum dulcamara

et al. 2016

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“…The Wrst carotenoid cleaving enzyme (Vp14) was isolated from the abscisic acid (ABA) deWcient viviparous maize mutant. Vp14 is an NCED and catalyses the rate-limiting step in ABA (also an apocarotenoid) biosynthesis, the cleavage of the 9-cis-isomer of neoxanthin or violaxanthin at the 11,12 position (Schwartz et al 1997). Based on the sequence homology to Vp14, nine CCDs have been identiWed in the Arabidopsis thaliana genome.…”

Section: Introductionmentioning

confidence: 99%

Cloning and characterisation of a maize carotenoid cleavage dioxygenase (ZmCCD1) and its involvement in the biosynthesis of apocarotenoids with various roles in mutualistic and parasitic interactions

Sun¹,

Hans

Walter

et al. 2008

Planta

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Colonisation of maize roots by arbuscular mycorrhizal (AM) fungi leads to the accumulation of apocarotenoids (cyclohexenone and mycorradicin derivatives). Other root apocarotenoids (strigolactones) are involved in signalling during early steps of the AM symbiosis but also in stimulation of germination of parasitic plant seeds. Both apocarotenoid classes are predicted to originate from cleavage of a carotenoid substrate by a carotenoid cleavage dioxygenase (CCD), but the precursors and cleavage enzymes are unknown. A Zea mays CCD (ZmCCD1) was cloned by RT-PCR and characterised by expression in carotenoid accumulating E. coli strains and analysis of cleavage products using GC-MS. ZmCCD1 eYciently cleaves carotenoids at the 9, 10 position and displays 78% amino acid identity to Arabidopsis thaliana CCD1 having similar properties. ZmCCD1 transcript levels were shown to be elevated upon root colonisation by AM fungi. Mycorrhization led to a decrease in seed germination of the parasitic plant Striga hermonthica as examined in a bioassay. ZmCCD1 is proposed to be involved in cyclohexenone and mycorradicin formation in mycorrhizal maize roots but not in strigolactone formation.

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“…Class 2 includes mutants associated with reduced or suppressed carotenoid accumulation in both endosperm and vegetative tissues (vp2, vp5, vp7, vp9, vp12, al1, y9 and w3) as a result of a mutational block in early biosynthetic steps before the branching point that separates ABA and carotenoid biosynthesis (Singh et al, 2003). Class 3 includes vp8 and vp14 which affect later steps of the biosynthesis (Schwartz et al, 1997) as well as vp10 and vp15 that regulate the synthesis of the molybdenum cofactor required for the last step in ABA biosynthesis (Porch et al, 2006;Suzuki et al, 2006). The relationship between dek33 and ABA has not yet been investigated.…”

Section: Introductionmentioning

confidence: 99%

Vivipary as a tool to analyze late embryogenic events in maize

et al. 2008

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In maize vivipary, the precocious germination of the seed while still attached to the ear, is the diagnostic phenotype of mutants, which are impaired in the biosynthesis or response to abscisic acid (ABA). Of the 15 genes so far described, 12 control specific steps in ABA biosynthesis, two mediate hormone response and one still has an undefined role. We have analyzed a collection of 25 independent vp isolates with the aim of determining the degree of mutational saturation that has so far been reached. Of the 25 viviparous mutants complementation tested, 22 correspond to known loci: six are allelic to vp1, another six to vp5, one to vp7, two to vp9, six to vp10 and one to w3. The remaining three represent genes not previously identified. All mutants so far tested except rea show a decrease in ABA content. As to the only two mutants (vp1 and rea) whose endogenous ABA content is not impaired, the reduction in sensitivity of the double mutant compared to the single ones suggests that the two genes control separate pathways in the ABA signal transduction. Some of the mutants in this collection have a characteristic incomplete germination that allows the embryo of the mature dry seed to resume germination. By exploiting this feature it is possible to infer, through a germination test, whether the mutant has been impaired in the acquisition of desiccation tolerance. This information provides the starting point for the dissection of the genetic basis of desiccation tolerance.

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Specific Oxidative Cleavage of Carotenoids by VP14 of Maize

Cited by 817 publications

References 18 publications

A Co-Opted Hormonal Cascade Activates Dormant Adventitious Root Primordia upon Flooding in Solanum dulcamara

A Co-Opted Hormonal Cascade Activates Dormant Adventitious Root Primordia upon Flooding in Solanum dulcamara

Cloning and characterisation of a maize carotenoid cleavage dioxygenase (ZmCCD1) and its involvement in the biosynthesis of apocarotenoids with various roles in mutualistic and parasitic interactions

Vivipary as a tool to analyze late embryogenic events in maize

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