Specific peptide patterns of follicular fluids at different growth stages analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Liu, Aixia; Zhu, Yimin; Luo, Qiong; Wu, Yanting; Gao, Huijuan; Zhu, Xiaoming; Xu, Chenming; Huang, He-feng

doi:10.1016/j.bbagen.2006.06.017

Cited by 24 publications

(18 citation statements)

References 31 publications

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“…To our knowledge, this study is the first to investigate a folate‐related follicle fluid proteome in human, while accounting for several important factors. We have obtained monofollicular fluids as the environment of a single oocyte and used a reproducible and validated proteomics technique to identify the folate‐related follicular proteome . Given the observational nature of the study, we cannot exclude that a high folate concentration in follicle fluid is a proxy for other unmeasured factors, such as an adequate dietary pattern.…”

Section: Discussionmentioning

confidence: 92%

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Preconception folic acid use influences the follicle fluid proteome

Twigt

Bezstarosti

Demmers

et al. 2015

Eur J Clin Investigation

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Section: Discussionmentioning

confidence: 92%

“…Monofollicular fluid total protein concentrations vary with follicular maturity . Therefore, concentrations of tHcy and B vitamins in monofollicular fluid are expressed in concentration per gram of protein.…”

Section: Methodsmentioning

confidence: 99%

Preconception folic acid use influences the follicle fluid proteome

Twigt

Bezstarosti

Demmers

et al. 2015

Eur J Clin Investigation

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“…Spitzer et al (1996) carried out 2-DE to compare protein profiles between mature and immature human follicles; their results indicated different FF protein contents in different developmental stages could be used as follicular maturation biomarkers. Liu et al (2007) performed MALDI-TOF-MS to analyze specific FF peptides of different developmental stages by hydrolyzing FF proteins and analyzing final products using MALDI-FOL-MS. A comparison of peaks obtained from samples at different development stages identified 5 specific peaks with a 100% occurrence in mature oocyte FF and 25 specific peaks with a 100% occurrence in immature oocyte FF. By searching the protein database, numerous proteins associated with oocyte maturation were identified.…”

Section: Discussionmentioning

confidence: 99%

Comparative Proteomic Analysis of Yak Follicular Fluid during Estrus

Guo

Pei

Ding

et al. 2015

Asian Australas. J. Anim. Sci

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The breeding of yaks is highly seasonal, there are many crucial proteins involved in the reproduction control program, especially in follicular development. In order to isolate differential proteins between mature and immature follicular fluid (FF) of yak, the FF from yak follicles with different sizes were sampled respectively, and two-dimensional gel electrophoresis (2-DE) of the proteins was carried out. After silver staining, the Image Master 2D platinum software was used for protein analysis and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) was performed for differential protein identification. The expression level of transferrin and enolase superfamily member 1 (ENOSF1) was determined by Western blotting for verification analysis. The results showed that 2-DE obtained an electrophoresis map of proteins from mature and immature yak FF with high resolution and repeatability. A comparison of protein profiles identified 12 differently expressed proteins, out of which 10 of them were upregulated while 2 were downregulated. Western blotting showed that the expression of transferrin and ENOSF1 was enhanced with follicular development. Both the obtained protein profiles and the differently expressed proteins identified in this study provided experimental data related to follicular development during yak breeding seasons. This study also laid the foundation for understanding the microenvironment during oocyte development.

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“…The differentially expressed proteins included fibrinogen g and antithrombin, which were downregulated in the FF fluid of women with recurrent spontaneous abortions; these data imply that coagulation factors play an important role in maintaining normal pegnancy. Liu et al (2007) studied FF samples obtained at different stages of follicular development by direct analysis of mixtures, using MALDI-TOF-MS. They identified specific peptide peaks that could be used to distinguish samples at different oocyte growth stages.…”

Section: Proteomic Analysis In Reproductive Medicinementioning

confidence: 99%

Mass spectrometry‐based proteomics in reproductive medicine

Kοlialexi¹,

Mavrou²,

Spyrou

et al. 2008

Mass Spectrometry Reviews

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The emergence of powerful mass spectrometry-based proteomic techniques has added a new dimension to the field of biomedical research. Application of these high throughput methodologies in pregnancy-related pathology has contributed to the comprehension of the underlying pathophysiologies and the successful identification of relevant protein biomarkers that can potentially change early diagnosis and treatment of several medical conditions related to human pregnancy. Most of the existing research on human reproduction and gestation has focused on follicular fluid, cervical/vaginal fluid, and amniotic fluid. Although proteome technologies in reproductive medicine research are not as yet widely applied, characterization of the proteome of reproductive fluids can be expected to significantly improve maternal healthcare. This article aims to summarize the applications of mass spectrometry based technology on the most important and specific biological fluids related to reproduction and gestation.

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Specific peptide patterns of follicular fluids at different growth stages analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Cited by 24 publications

References 31 publications

Preconception folic acid use influences the follicle fluid proteome

Preconception folic acid use influences the follicle fluid proteome

Comparative Proteomic Analysis of Yak Follicular Fluid during Estrus

Mass spectrometry‐based proteomics in reproductive medicine

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