Specific Primers for Rapid Typing of Tuber Borchii Mycorrhizal Roots

Mello, Antonietta; Garnero, Lilia; Meotto, F.; Bonfante, Paola

doi:10.17660/actahortic.1998.457.28

Cited by 7 publications

(4 citation statements)

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“…with ITS sequence motifs is consistent with investigations made using traditional approaches (i.e., by morphology), as well as through molecular fingerprinting and/or phylogeny of genomic loci such as ITS and ␤-tubulin (see GenBank entries) (29,31,35,36,38,40,(51)(52)(53)56). The specific motifs identified the Tuber species not only from the ascocarp sequences but also from the sequences of unidentified soil-borne ectomycorrhizas (either complete or partial), suggesting that the ITS motifs can be used in a wide range of ecological studies to monitor the hypogeous Tuber species without cultivation.…”

Section: Vol 73 2007 Its Sequence Motifs For Truffle Identificationsupporting

confidence: 85%

“…The isolates were collected from different countries in Europe and Asia and have comprehensive, full-length ITS sequences (i.e., Ͼ460 bp). They were clearly identified from the ascocarps by morphology as well as by molecular fingerprinting and phylogenetic inference, including single nucleotide polymorphism and haplotype genotyping of several loci, such as ITS, ␤-tubulin, translation elongation factor 1-alfa, and protein kinase C (29,31,34,36,38,40,(51)(52)(53)56). The isolates also showed high sequence homology with their corresponding reference and well-identified sequences in our groups as well as in other groups: accession no.…”

Section: Methodssupporting

confidence: 54%

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Identification of Internal Transcribed Spacer Sequence Motifs in Truffles: a First Step toward Their DNA Bar Coding

Karkouri

Murat

Zampieri

et al. 2007

Appl Environ Microbiol

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This work presents DNA sequence motifs from the internal transcribed spacer (ITS) of the nuclear rRNA repeat unit which are useful for the identification of five European and Asiatic truffles (Tuber magnatum, T. melanosporum, T. indicum, T. aestivum, and T. mesentericum). Truffles are edible mycorrhizal ascomycetes that show similar morphological characteristics but that have distinct organoleptic and economic values. A total of 36 out of 46 ITS1 or ITS2 sequence motifs have allowed an accurate in silico distinction of the five truffles to be made (i.e., by pattern matching and/or BLAST analysis on downloaded GenBank sequences and directly against GenBank databases). The motifs considered the intraspecific genetic variability of each species, including rare haplotypes, and assigned their respective species from either the ascocarps or ectomycorrhizas. The data indicate that short ITS1 or ITS2 motifs (<50 bp in size) can be considered promising tools for truffle species identification. A dot blot hybridization analysis of T. magnatum and T. melanosporum compared with other close relatives or distant lineages allowed at least one highly specific motif to be identified for each species. These results were confirmed in a blind test which included new field isolates. The current work has provided a reliable new tool for a truffle oligonucleotide bar code and identification in ecological and evolutionary studies.The internal transcribed spacers (ITS) of nuclear rRNA repeat units have frequently been used to identify fungal species in fields (9,11,14,20). The emergence of sequence-based identification with a BLAST similarity search connected to public databases (1, 25) has resolved several experimental and taxonomic constraints. However, generalized databases can contain either misidentified or misannotated sequences (7,17,23,24). In addition, BLAST outcomes give no information about species delimitation for closely related species. The identification of unknown ITS sequences based on these approaches therefore needs to be supported by phylogenetic analysis (19,20).At the same time, the ITS offers sequence motifs that are useful for the development of fungal DNA bar coding. This is a technique that uses short DNA sequences from a standard-

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Section: Vol 73 2007 Its Sequence Motifs For Truffle Identificationsupporting

confidence: 85%

Section: Methodssupporting

confidence: 54%

Identification of Internal Transcribed Spacer Sequence Motifs in Truffles: a First Step toward Their DNA Bar Coding

Karkouri

Murat

Zampieri

et al. 2007

Appl Environ Microbiol

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“…The finding of a substantial polymorphism in ITS regions questions the efficiency of primers designed on their sequences. However, this is not an issue for some ectomycorrhizal species: Tuber species possess homogenous ITS sequences, a low RAPD polymorphism ( Lanfranco et al 1995 ) and specific primers designed on ITS sequences are highly efficient ( Amicucci et al 1998 ; Mello et al 1998 ).…”

Section: Discussionmentioning

confidence: 99%

Intrasporal variability of ribosomal sequences in the endomycorrhizal fungus Gigaspora margarita

Lanfranco¹,

Delpero

Bonfante³

1999

Molecular Ecology

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106

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The sequence variability of the ribosomal internal transcribed spacer (ITS) region, which comprises the 5.8 gene and the flanking regions ITS1 and ITS2, was investigated in the arbuscular mycorrhizal fungus Gigaspora margarita. DNA analysis of a multispore preparation and three single spores led to the identification of 11 slightly different sequences (three variants within a single spore), indicating substantial intersporal and intrasporal genetic variability (up to 9% sequence divergence). The sequence variations inside a single spore may be higher than that observed between spores. Even so, primers designed on the ITS1 and ITS2 regions identified Gi. margarita isolates and detected the endophyte during colonization.

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“…and other fungi with S. brunnea (Bertini et al 1998;Mello et al 1998;Amicucci et al 2000;Zambonelli et al 2000). Palazón et al (2005) began experiments on fungicide treatments in vitro and in situ for the purpose of controlling S. brunnea contamination in nurseries.…”

Section: Introductionmentioning

confidence: 99%

Effect ofSphaerosporella brunneamycorrhizas on mycorrhization ofQuercus ilex×Tuber melanosporum

García-Montero¹,

Massimo²,

Manjón³

et al. 2008

New Zealand Journal of Crop and Horticultural Science

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It is generally accepted that Sphaerosporella brunnea is a significant ectomycorrhizal contaminant in nurseries producing plants mycorrhized with various species of Tuber, and subsequently in truffières after outplanting. At the University of Alcalá, Spain, 397 small plants of Quercus ilex which were mycorrhized with Tuber melanosporum were inadvertently contaminated with S. brunnea, and this contamination was then monitored for 2 years. Sixty percent of the plants were contaminated and had S. brunnea ascomata on the surface of the container on one or several occasions. However, a Spearman test provided no evidence that S. brunnea mycorrhizas affected T. melanosporum mycorrhization whereas other contaminating ectomycorrhizal fungi significantly did. Therefore, it appears that S. brunnea is not detrimental to plants which are well mycorrhized with T. melanosporum.

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Specific Primers for Rapid Typing of Tuber Borchii Mycorrhizal Roots

Cited by 7 publications

References 0 publications

Identification of Internal Transcribed Spacer Sequence Motifs in Truffles: a First Step toward Their DNA Bar Coding

Identification of Internal Transcribed Spacer Sequence Motifs in Truffles: a First Step toward Their DNA Bar Coding

Intrasporal variability of ribosomal sequences in the endomycorrhizal fungus Gigaspora margarita

Effect ofSphaerosporella brunneamycorrhizas on mycorrhization ofQuercus ilex×Tuber melanosporum

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