Specific proteomic adaptation to distinct environments in Vibrio parahaemolyticus includes significant fluctuations in expression of essential proteins

Abstract

Bacteria constantly experience changes to their external milieu and need to adapt accordingly to ensure their survival. Certain bacteria adapt by means of cellular differentiation, resulting in the development of a specific cell type that is specialized for life in a distinct environment. Furthermore, to understand how bacteria adapt, it is essential to appreciate the significant changes that occur at the proteomic level. By analysing the proteome of our model organism Vibrio parahaemolyticus from distinct environmental conditions and cellular differential states, we demonstrate that the proteomic expression profile is highly flexible, which likely allows it to adapt to life in different environmental conditions and habitats. We show that, even within the same swarm colony, there are specific zones of cells with distinct expression profiles. Furthermore, our data indicate that cell surface attachment and swarmer cell differentiation are distinct programmes that require specific proteomic expression profiles. This likely allows V. parahaemolyticus to adapt to life in different environmental conditions and habitats. Finally, our analyses reveal that the expression profile of the essential protein pool is highly fluid, with significant fluctuations that dependent on the specific lifestyle , environment and differentiation state of the bacterium.

Adjustment in the Composition and Organization of Proteus mirabilis Lipids during the Swarming Process

“…Only Rauprich et al [32] indicated that the leading edge of P. mirabilis migrating cells was quite ragged in appearance. However, the encroaching swarm fronts of the following strains Serratia liquefaciens MG1 [39], B. subtilis JCM 32,485 [40], Bacillus cereus NCIB 8122 [41], and Vibrio parahaemolyticus RIMD 2,210,633 [42] also resembled finger-like structures migrating outward towards the colony border.…”

Impact of temperature on the virulence of Streptococcus agalactiae in Indonesian aquaculture: A better vaccine design is required

“…Acidophile (pH 0.5 to 5) Efficient efflux system, acid tolerant membrane, proton exclusion and secondary transporters driven by protons [45,46] Halorhodospira halochloris, Thiohalospira alkaliphila, Bacillus firmus, Natronomonas pharaonis Alcaliphile (pH 8.5 to 11) Efficient proton uptake system mediated by antiporters of the membrane, OH-ion [45] Psychrobacter adeliensis, P. aestuarii, P. alimentarius, P aquimaris, P. luti, P glacincola Psychrophile (-10°C-40°C) Synthesis of unsaturated fatty acids to prevent the decrease of membrane fluidity reduces the size of the cell and elevates cellular water in an ordered fashion [47][48][49] Serratia ureilytica Thermophile (20°C-54°C) Modified vegetative cells into resistant structures [50] Available at www.veterinaryworld.org/Vol.17/March-2024/20.pdf by changing into different cell types (swarm cells) to maintain optimal growth and motility [57,58]. This adaptation involves a number of genes involved in their expression and repression.…”

Microbial adaptation to different environmental conditions: molecular perspective of evolved genetic and cellular systems

“…The bacteria can also become resistant to antibiotics due to the mutations in their genome or by uptaking foreign DNA (Larsson and Flach 2021). The microbes can also adapt to the changing environmental condition by transforming to different cell types that help the microbes to maintain optimum growth in new environment (Freitas et al 2020). An example of this changing environment adaptability towards the changing surrounding support is reported in Vibrio parahaemolyticus (Freitas et al 2020).…”

“…The microbes can also adapt to the changing environmental condition by transforming to different cell types that help the microbes to maintain optimum growth in new environment (Freitas et al 2020). An example of this changing environment adaptability towards the changing surrounding support is reported in Vibrio parahaemolyticus (Freitas et al 2020). In a highly viscous vicinity, the bacteria form a swarmer cell that ensures motility for the process of chemotaxis (Jose and Singh 2020).…”