Specific Real-Time PCR for the Detection and Absolute Quantitation of Grapevine Roditis Leaf Discoloration-Associated Virus, an EPPO Alert Pathogen

Morán, Félix; Sassalou, Chrysoula-Lito; Canales, Célia; Maliogka, Varvara I.; Olmos, Antonio; Ruiz-García, Ana Belén

doi:10.3390/plants9091151

Cited by 4 publications

(2 citation statements)

References 21 publications

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“…To detect GRLDaV, a sensitive and specifi c quantitative real-time PCR TaqMan was developed (Morán et al, 2020). This real-time PCR detected all known GRLDaV isolates, regardless of their high degree of genetic diversity, from different geographic locations.…”

Section: Introductionmentioning

confidence: 99%

“…This real-time PCR detected all known GRLDaV isolates, regardless of their high degree of genetic diversity, from different geographic locations. In addition, it was successfully used for the quantitative determination of GRLDaV in the vegetative material and in two (artifi cially contaminated) species of mealybugs -Planococcus citri and Pseudococcus viburni, which transmitted the virus under experimental conditions (Morán et al, 2020). The virus can be artifi cially transmitted to V. vinifera as well as to herbaceous plants Chenopodium quinoa, Gomphrena globosa, and Nicotiana benthamiana (EPPO Alert List, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Grapevine roditis leaf Discoloration-associated virus: express pest risk analysis for Ukraine

et al. 2022

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Aim. To conduct an express pest risk analysis of Grapevine Roditis leaf discoloration-associated virus (GRLDaV) for Ukraine, a virus that has been related to a grapevine disease and was included in the EPPO Alert List in 2018. Methods. The phytosanitary risk analysis was carried out on the basis of an analytical review of expert literature and in accordance with the EPPO Decision-support scheme for an Express Pest Risk Analysis (EPPO, 2012) and with methodological recommendations for Ukraine (Pylypenko et al, 2012). The possibility of further spread and the potential range of the virus were determined using modern software packages AgroAtlas (Afonin and Li, 2011; Shumilin and Li, 2009), MapInfo Pro15.0 (ESTIMap®) and IDRISI SELVA (Clarklabs®). Results. An express pest risk analysis of GRLDaV for Ukraine was carried out for the first time. Outbreaks of the virus were first detected in the 1980ies in Greece (Rumbos, Avgelis, 1989) and from 2014–2018 in: Italy (Chiumenti et al, 2015, 2016; Maliogka et al, 2015), Turkey (Adan, 2016; Serçe et al, 2018) and Croatia (Vončina et al, 2018). Climatic predictors were analyzed in the outbreaks of the countries where the causal agent of the disease was identified. The potential range of GRLDaV in Ukraine has been established in case of importing infected planting material with further spreading of the virus. Risk management measures are proposed, which envisage including GRLDaV in the list of the Regulated Non-Quarantine Harmful Organisms of Ukraine. Conclusions. There is a possibility of introduction, further spread, and harmfulness of Grapevine Roditis leaf discoloration-associated virus in Ukraine, which is due to the presence of the host plant (grapevine, Vitis vinifera ssp. vinifera L.) and the corresponding climatic conditions of the southern and (part of) western Ukraine, where grapevine is cultivated on an industrial scale. The introduction of GRLDaV into Ukraine is possible as a result of the import of GRLDaV-infected grapevine planting material from the countries where the virus has been reported and presumably is still present. The current Ukrainian phytosanitary measures cannot reliably prevent the risk of intoduction of GRLDaV into Ukraine. The inclusion of GRLDaV in the List of the Regulated Non-Quarantine Harmful Organisms of Ukraine with the recommendation of permission to import grapevine planting material certified for the absence of GRLDaV (from the countries where the virus is reported) or mandatory testing of imported grapevine planting material for the presence of GRLDaV (from areas where such certification is absent), can be an effective risk management measure. It requires the producers and importers of grapevine planting material to include GRLDaV virus in their certification schemes to prevent eventual further spreading of the virus. It is recommended to do a nation-wide survey to determine the likelihood of the presence of GRLDaV in the region. Further research to identify possible natural virus vectors and to develop methods of (latent) GRLDaV diagnostics are needed. Finally additional studies on prevention and control of the virus (including breeding for resistance) are also required.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Grapevine roditis leaf Discoloration-associated virus: express pest risk analysis for Ukraine

et al. 2022

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Improved detection, mealybug transmission and prevalence of grapevine Roditis leaf discoloration-associated virus in Greek vineyards

Sassalou,

Panailidou,

Chiumenti

et al. 2023

J Plant Pathol

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Grapevine Roditis leaf discoloration-associated virus (GRLDaV) is a member of the species Badnavirus decoloratiovitis in the genus Badnavirus that was recently identified in grapevine using high throughput sequencing. In this study, a large-scale survey was conducted to monitor the prevalence of the virus in major viticultural areas of Greece. A total of 970 samples were collected from vines and molecularly analysed using PCR assays targeting two regions of the virus genome. GRLDaV was detected in 8 geographic areas of Greece, showing high frequency in southern and central Greece and an overall prevalence of 17.1%. GRLDaV was identified mainly in autochthonous grapevine varieties, and it showed higher frequency in the self-rooted ones, especially in the geographic region of the Cyclades islands. In transmission tests conducted using Planococcus ficus (Signoret) and 48 h acquisition access period (AAP) and inoculation access period (IAP), the virus was transmitted with an efficiency of 14.3% (3/21 grapevines). Finally, electron microscopy revealed the bacilliform morphology of GRLDaV virions, thus further confirming its episomal infection.

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Genetic diversity and population structure of grapevine Roditis leaf discoloration‐associated virus in Turkey vineyards

Ulubaş‐Serçe,

Aydınlık,

Öztürk

et al. 2024

Journal of Phytopathology

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Grapevine Roditis leaf discoloration‐associated virus (GRLDaV) is a type of virus that infects grapevines. This research aimed to determine the prevalence of GRLDaV in Turkey vineyards and investigate the genetic diversity and population structure of the selected viral isolates from Turkey compared to isolates from other countries. In this study, 800 shoot samples from Turkey vineyards were collected in late spring and autumn from different regions. The samples were analysed by RT‐PCR targeting the partial RNaseH‐like gene region of the virus genome. The overall prevalence of GRLDaV was 8.1% among the samples. Phylogenetic analysis of the GRLDaV genome showed that the GRLDaV isolates were clustered into two major lineages. The nucleotide diversity (π) value of Group I, including only Mediterranean isolates, was lower than Group II, suggesting little variation among isolates. Even though π values were low among the geographical regions in Turkey, the region with the highest nucleotide diversity was the Aegean population, followed by the Eastern and Mediterranean populations. Comparison between nonsynonymous and synonymous substitutions showed that negative selection acts on synonymous amino acids. Along with neutrality tests and gene flow analyses, it was emphasized that genetic drift played an important role in shaping the GRLDaV populations. This study provides important information on the prevalence and genetic diversity of GRLDaV in Turkey vineyards. The findings will help to develop better strategies to manage and control this virus, which can cause significant economic losses in grape industries.

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Specific Real-Time PCR for the Detection and Absolute Quantitation of Grapevine Roditis Leaf Discoloration-Associated Virus, an EPPO Alert Pathogen

Cited by 4 publications

References 21 publications

Grapevine roditis leaf Discoloration-associated virus: express pest risk analysis for Ukraine

Grapevine roditis leaf Discoloration-associated virus: express pest risk analysis for Ukraine

Improved detection, mealybug transmission and prevalence of grapevine Roditis leaf discoloration-associated virus in Greek vineyards

Genetic diversity and population structure of grapevine Roditis leaf discoloration‐associated virus in Turkey vineyards

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