Specific removal of DNA antibodies in vivo by extracorporeal circulation over DNA immobilized in collodion charcoal

Terman, David S.; Petty, Donald; Harbeck, Ronald J.; Carr, Ronald I.; Buffaloe, George

doi:10.1016/0090-1229(77)90095-2

Cited by 35 publications

(24 citation statements)

References 18 publications

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“…However, the most effective treatment is probably the selective removal of specific pathogenic antibodies from the blood. In line with this point of view, Terman et al reported the specific removal of DNA antibodies by extracorporeal circulation over DNA immobilized in collodion charcoal and cellulose agar (5,6). They combined DNA with matrices, charcoal, and cellulose agar, which appears not to be tightly bonded.…”

Section: Introductionmentioning

confidence: 84%

THE REMOVAL OF ANTI‐DNA ANTIBODIES IN VITRO USING DNA COMBINED IMMUNOADSORBENT MATRICES

Taneda

Miyazawa

Ogawa

et al. 1984

The Journal of Dermatology

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An important approach to the treatment of auto-immune diseases is the selective removal of specifically pathogenic antibodies from the blood. This study reports the effectiveness of using DNAcombined immuno-absorbent matrixes for the removal of anti-DNA antibodies in vitro. Sera obtained from patients with Systemic Lupus Erythematosus (SLE) were used, as the pathogenesis of SLE is partially mediated by antibodies against DNA (1). A series of DNA-combined immunoabsorbent matrices was prepared using several different approaches, and the adsorption of anti-DNA antibodies from the SLE sera was investigated on these matrices. A comparison was then made between these matrices and commercially available adsorbents with respect to their adsorbing abilities and their stability in sera. Readings of the following were also made before and after the reaction; pH, albumin, globulin, CH so , compliments C" C., c, DNA, DNA and anti-DNA antibodies complex ratios. Of the adsorbents investigated, the newly prepared immuno-adsorbent matrix of DNA, glow discharge treated DNA-PVA on cotton gauze, proved to be the most effective in reducing the anti-DNA antibodies and immune complex levels specifically, while remaining relatively non-toxic to the host.

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Section: Introductionmentioning

confidence: 84%

THE REMOVAL OF ANTI‐DNA ANTIBODIES IN VITRO USING DNA COMBINED IMMUNOADSORBENT MATRICES

Taneda

Miyazawa

Ogawa

et al. 1984

The Journal of Dermatology

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“…This was initially used to increase the blood compatibility of the adsorbent artificial cells for hemoperfusion (Chang, 1969a). In addition, albumin-coated collodion-activated charcoal (ACAC) was found to effectively remove antibodies to albumin in animal studies (Terman et al, 1977). This has become the basis of one line of research in which other types of antigens or antibodies are applied to the collodion coating of the activated charcoal to form immunosorbents.…”

Section: Surface Modification In Immunoadsorptionmentioning

confidence: 99%

“…This albumin coating has also been applied to synthetic immunosorbents resulting in blood compatible syn thetic blood group immunosorbents (Chang, 1980d). In addition, Terman et al (1977) show in animal studies that albumin-coated collodion-activated charcoal (ACAC) can remove antibodies to albumin. This has become a basis of one line of his research in which other types of antigens or antibodies are applied to the collodion coating of the artificial cells to form immunosorbents ( Fig.…”

Section: Surface Modification In Immunoadsorptionmentioning

confidence: 99%

First Design and Clinical Use in Patients of Surface Modified Sorbent Hemoperfusion Based on Artificial Cells for Poisoning, Kidney Failure, Liver Failure and Immunology

Chang¹

2017

Hemoperfusion, Plasmaperfusion and Other Clinical Uses of General, Biospecific, Immuno and Leucocyte Adsorbents

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“…adsorption on a synthetic immunoadsorbent [1], on collodion-charcoal [26], or red cell ghosts [3]. However, these procedures lead to several disadvantageous effects.…”

Section: Complication Of Antibody Removal Therapy and Exchange Plasmamentioning

confidence: 99%

A New Therapeutic Antibody Removal Method Using Antigen Positive Red Cells

Yoshida¹,

Yoshida²,

Tatsumi³

et al. 1982

Vox Sanguinis

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A study was made for the therapeutic removal of red cell antibodies using antigen positive red cells. With preliminary experiments in vitro, the criteria for the optimal absorption of anti-M with O MM red cells were estimated to be the following: an incubation time of 10 min, an incubation temperature of 0-4 °C (in an ice-water bath), and a red cell-to-plasma ratio of 1:2.5. On the basis of these in vitro experiments, the following procedure was designed. One unit (about 90 ml) of O MM packed red cells was added to a bag containing 250 ml of patient’s plasma, the mixture was incubated in an ice-water bath for 10 min. Following centrifugation, autologous plasma from which the antibodies were removed was reinfused. After absorption the titer of anti-M fell from 512 to 4. No variation in the level of other plasma components was detected and no hemolysis was seen. After 2.5 l of patient’s plasma was treated using the above described method, the antibody titer usually decreased one log(2) unit. When this method was compared with exchange plasmapheresis, no significant abnormalities in the immunoglobulin and hemostatic factors could be detected, but a moderate decrease in platelet count was seen after plasmapheresis. Thus, it appears that our method provides a rational therapeutic modality for specifically removing antibodies.

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Specific removal of DNA antibodies in vivo by extracorporeal circulation over DNA immobilized in collodion charcoal

Cited by 35 publications

References 18 publications

THE REMOVAL OF ANTI‐DNA ANTIBODIES IN VITRO USING DNA COMBINED IMMUNOADSORBENT MATRICES

THE REMOVAL OF ANTI‐DNA ANTIBODIES IN VITRO USING DNA COMBINED IMMUNOADSORBENT MATRICES

First Design and Clinical Use in Patients of Surface Modified Sorbent Hemoperfusion Based on Artificial Cells for Poisoning, Kidney Failure, Liver Failure and Immunology

A New Therapeutic Antibody Removal Method Using Antigen Positive Red Cells

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