Specific roles of Os4BGlu10, Os6BGlu24, and Os9BGlu33 in seed germination, root elongation, and drought tolerance in rice

Ren, Ruijuan; Dong, Liang; ChunYan, Zhen; Chen, Defu; Chen, Xiwen

doi:10.1007/s00425-019-03125-2

Cited by 23 publications

(14 citation statements)

References 39 publications

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“…In the case of drought stress, simulated by PEG osmotic stress, roots and shoots of the β-glucosidase expression lines were significantly longer than those of wild type, as well ( Figure 5 B). An Os4BGlu10 knockout rice line characterized by Ren and colleagues [ 30 ] showed decreased tolerance to salt and drought stress, consistent with the expression of Os4BGlu10 and its homologues in Arabidopsis improving salt stress resistance.…”

Section: Discussionsupporting

confidence: 53%

Action of Multiple Rice β-Glucosidases on Abscisic Acid Glucose Ester

Kongdin

Mahong

Lee

et al. 2021

IJMS

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Conjugation of phytohormones with glucose is a means of modulating their activities, which can be rapidly reversed by the action of β-glucosidases. Evaluation of previously characterized recombinant rice β-glucosidases found that nearly all could hydrolyze abscisic acid glucose ester (ABA-GE). Os4BGlu12 and Os4BGlu13, which are known to act on other phytohormones, had the highest activity. We expressed Os4BGlu12, Os4BGlu13 and other members of a highly similar rice chromosome 4 gene cluster (Os4BGlu9, Os4BGlu10 and Os4BGlu11) in transgenic Arabidopsis. Extracts of transgenic lines expressing each of the five genes had higher β-glucosidase activities on ABA-GE and gibberellin A4 glucose ester (GA4-GE). The β-glucosidase expression lines exhibited longer root and shoot lengths than control plants in response to salt and drought stress. Fusions of each of these proteins with green fluorescent protein localized near the plasma membrane and in the apoplast in tobacco leaf epithelial cells. The action of these extracellular β-glucosidases on multiple phytohormones suggests they may modulate the interactions between these phytohormones.

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Section: Discussionsupporting

confidence: 53%

Action of Multiple Rice β-Glucosidases on Abscisic Acid Glucose Ester

Kongdin

Mahong

Lee

et al. 2021

IJMS

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“…After 14 d grown in the glasshouse, the seedlings were drought‐treated by not watering for 7 d, and then watered for 7 d (rehydration). The survival rates, chlorophyll fluorescence variable fluorescence/maximal fluorescence ( F v / F m ) parameters and relative water content (RWC) were determined according to the methods described in our previous research (Ren et al , ) using an ultra‐portable chlorophyll fluorimeter Mini‐PAM‐II (Walz, Nuremberg, Germany).…”

Section: Methodsmentioning

confidence: 99%

Chloroplastic Os3BGlu6 contributes significantly to cellular ABA pools and impacts drought tolerance and photosynthesis in rice

Chen

Dong

et al. 2020

New Phytologist

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Cellular abscisic acid (ABA) concentration is determined by both de novo biosynthesis and recycling via b-glucosidase(s). However, which rice b-glucosidase(s) are involved in this process remains unknown. Here, we report on a chloroplastic b-glucosidase isoenzyme, Os3BGlu6, that functions in ABA recycling in rice.Disruption of Os3BGlu6 in rice resulted in dwarfism, lower ABA content in leaves, droughtsensitivity, lower photosynthesis rate and higher intercellular CO 2 concentration. Os3BGlu6 could hydrolyze ABA-GE to ABA in vitro. The reversion and overexpression rice lines restored or increased the drought tolerance as shown by the higher b-glucosidase activity, ABA concentrations and expressions of ABA-and drought-responsive genes. Drought induced Os3BGlu6 to form dimers, and the degree of polymerization correlated well with the increase in cellular ABA concentrations and drought tolerance in rice.Os3BGlu6 was responsive to drought and ABA treatments, and the protein was localized to the chloroplast. Disruption of Os3BGlu6 resulted in the increased stomatal density and impaired stomatal movement. Transcriptomics revealed that disruption of Os3BGlu6 resulted in chloroplastic oxidative stress and lowered Rubisco activity even under normal conditions. Taken together, these results suggest that chloroplastically localized Os3BGlu6 significantly affects cellular ABA pools, thereby affecting drought tolerance and photosynthesis in rice.

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“…OsPP2CC51 is down-regulated in japonica 02428, consistent with our results that japonica lines have a higher GI. Mutants of Os9BGlu33 exhibit a lower germination rate than wild type seeds, but which are less inhibited than wild type in the presence of ABA, a germination inhibitor (Ren et al 2019).…”

Section: Differential Expression Of Seed Dormancy-related Genes In Japonica and Indica Ricementioning

confidence: 97%

Integrating GWAS and transcriptomics to identify genes involved in seed dormancy in rice

et al. 2021

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Seed dormancy is a key agricultural trait to prevent pre-harvest sprouting in crop plants such as rice (Oryza sativa L.), wheat (Triticum aestivum), and barley (Hordeum vulgare L.). However, our knowledge of seed dormancy is hampered by the complexities of studying a trait that changes over time after seed harvest, and is complicated by interactions between phytohormones, other regulators, and the environment. Here, we have conducted a genome-wide association study using a panel of 311 natural accessions of cultivated rice, examining a total of 519,158 single nucleotide polymorphisms (SNPs).Eight quantitative trait loci (QTLs) were found to associate with seed dormancy; expression of candidate genes within 100 kb of each QTL was examined in two published, germination-speci c transcriptomic datasets. Ten candidate genes, differentially expressed within the rst four days post-imbibition, were identi ed. Five of these genes had previously been associated with awn length, heading date, yield, and spikelet length phenotypes. In addition, previously identi ed genes involved in hormone signaling during germination were found to be differentially expressed between a japonica and an indica line; SNPs in the promoter of Os9BGlu33 were associated with germination index. Collectively, our results are useful for future characterization of seed dormancy mechanism and crop improvement, and suggest haplotypes for further analysis that may be of use to boost PHS resistance in rice. Key MessagesSeveral QTLs and genes responsible for seed dormancy were detected and SNP candidates were shown to cause changes in seed germination.

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Specific roles of Os4BGlu10, Os6BGlu24, and Os9BGlu33 in seed germination, root elongation, and drought tolerance in rice

Cited by 23 publications

References 39 publications

Action of Multiple Rice β-Glucosidases on Abscisic Acid Glucose Ester

Action of Multiple Rice β-Glucosidases on Abscisic Acid Glucose Ester

Chloroplastic Os3BGlu6 contributes significantly to cellular ABA pools and impacts drought tolerance and photosynthesis in rice

Integrating GWAS and transcriptomics to identify genes involved in seed dormancy in rice

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