Specific Strains of Honeybee Gut
            <i>Lactobacillus</i>
            Stimulate Host Immune System to Protect against Pathogenic Hafnia alvei

Lang, Haoyu; Duan, Huijuan; Wang, Jieni; Zhang, Wenhao; Guo, Jun; Zhang, Xue; Hu, Xiaosong; Zheng, Hao

doi:10.1128/spectrum.01896-21

Cited by 58 publications

(58 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Within this megadiverse insect order, a large number of species engage in different feeding habits and ecological habitats, yet there is still a lack of comprehensive investigation into the association with bacterial partners. The gut microbiota of insects, as well as other animals, provide the host with key functions in diet digestion [ 53 , 54 ], abiotic stress detoxification [ 55 ], nutrition supplementation [ 56 ], pathogen resistance [ 57 , 58 ], etc. In this work, insects of three heteropteran infraorders were selected for the characterization of their gut microbiota.…”

Section: Discussionmentioning

confidence: 99%

The Impact of Environmental Habitats and Diets on the Gut Microbiota Diversity of True Bugs (Hemiptera: Heteroptera)

Sun

Meng

et al. 2022

Biology

Self Cite

View full text Add to dashboard Cite

Insects are generally associated with gut bacterial communities that benefit the hosts with respect to diet digestion, limiting resource supplementation, pathogen defense, and ecological niche expansion. Heteroptera (true bugs) represent one of the largest and most diverse insect lineages and comprise species consuming different diets and inhabiting various ecological niches, even including underwater. However, the bacterial symbiotic associations have been characterized for those basically restricted to herbivorous stink bugs of the infraorder Pentatomomorpha. The gut microbiota associated with the megadiverse heteropteran lineages and the implications of ecological and diet variance remain largely unknown. Here, we conducted a bacterial 16S rRNA amplicon sequencing of the gut microbiota across 30 species of true bugs representative of different ecological niches and diets. It was revealed that Proteobacteria and Firmicute were the predominant bacterial phyla. Environmental habitats and diets synergistically contributed to the diversity of the gut bacterial community of true bugs. True bugs living in aquatic environments harbored multiple bacterial taxa that were not present in their terrestrial counterparts. Carnivorous true bugs possessed distinct gut microbiota compared to phytophagous species. Particularly, assassin bugs of the family Reduviidae possessed a characterized gut microbiota predominantly composed of one Enterococcus with different Proteobacteria, implying a specific association between the gut bacteria and host. Overall, our findings highlight the importance of the comprehensive surveillance of gut microbiota association with true bugs for understanding the molecular mechanisms underpinning insect–bacteria symbiosis.

show abstract

Section: Discussionmentioning

confidence: 99%

The Impact of Environmental Habitats and Diets on the Gut Microbiota Diversity of True Bugs (Hemiptera: Heteroptera)

Sun

Meng

et al. 2022

Biology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Moreover, Lactiplantibacillus plantarum can disturb the natural symbiotic microbiota and significantly increase the mortality of Nosema ‐infected honeybees (Ptaszynska et al ., 2016). Several bacteria strains isolated from the honeybee gut have been demonstrated to be protective against pathogenic microorganisms (Carina Audisio et al ., 2011; Miller et al ., 2021; Lang et al ., 2022). More understanding of the microbial community of bumblebees is urgently needed to improve bumblebee health and to provide theoretical support for the conservation of commercial and wild bumblebees.…”

Section: Potential Roles Of the Gut Microbiota In Pathogen Resistancementioning

confidence: 99%

Bumblebees with the socially transmitted microbiome: A novel model organism for gut microbiota research

Zhang

Zheng

2022

Insect Science

Self Cite

View full text Add to dashboard Cite

Eusocial bumble and honey bees are important pollinators for global ecology and the agricultural economy. Although both the bumble and honey bees possess similar and host‐restricted gut microbiota, they differ in aspects of morphology, autonomy, physiology, behavior, and life cycle. The social bee gut bacteria exhibit host specificity that is likely a result of long‐term co‐evolution. The unique life cycle of bumblebees is key for the acquisition and development of their gut microbiota, and affects the strain‐level diversity of the core bacterial species. Studies on bumblebee gut bacteria show that they retain less functional capacity for carbohydrate metabolism compared with that of the honeybee. We discuss the potential roles of the bumblebee gut microbiota against pathogenic threats and the application of host‐specific probiotics for bumblebees. Given the advantages of the bumblebee microbiome, including the simple structure and host specificity, and the ease of manipulating bumblebee colonies, we propose that bumblebees may provide a valuable system for understanding the general principles of host–microbe interactions, gut–brain axis, and vertical transmission.

show abstract

“…Studies have shown that honeybee gut microbiota affects host nutrition, weight gain, and endocrine signaling [20]. Moreover, gut microbial members also have immunomodulation effects on bees [21] and protect honeybees from the opportunistic pathogens [22], which is critical to their survival and health in a complex environment [23]. Furthermore, there are known interaction between honeybee intestinal microorganisms and some honeybee diseases, for example, Nosema ceranae infection can promote proliferation of yeasts in honeybee gut and cause disease [24].…”

Section: Introductionmentioning

confidence: 99%

Strain-level profiling with picodroplet microfluidic cultivation reveals host-specific adaption of honeybee gut symbionts

Meng

Zhang

et al. 2022

Microbiome

Self Cite

View full text Add to dashboard Cite

Background Symbiotic gut microbes have a rich genomic and metabolic pool and are closely related to hosts’ health. Traditional sequencing profiling masks the genomic and phenotypic diversity among strains from the same species. Innovative droplet-based microfluidic cultivation may help to elucidate the inter-strain interactions. A limited number of bacterial phylotypes colonize the honeybee gut, while individual strains possess unique genomic potential and critical capabilities, which provides a particularly good model for strain-level analyses. Results Here, we construct a droplet-based microfluidic platform and generated ~ 6 × 108 droplets encapsulated with individual bacterial cells from the honeybee gut and cultivate in different media. Shotgun metagenomic analysis reveals significant changes in community structure after droplet-based cultivation, with certain species showing higher strain-level diversity than in gut samples. We obtain metagenome-assembled genomes, and comparative analysis reveal a potential novel cluster from Bifidobacterium in the honeybee. Interestingly, Lactobacillus panisapium strains obtained via droplet cultivation from Apis mellifera contain a unique set of genes encoding l-arabinofuranosidase, which is likely important for the survival of bacteria in competitive environments. Conclusions By encapsulating single bacteria cells inside microfluidic droplets, we exclude potential interspecific competition for the enrichment of rare strains by shotgun sequencing at high resolution. The comparative genomic analysis reveals underlying mechanisms for host-specific adaptations, providing intriguing insights into microbe-microbe interactions. The current approach may facilitate the hunting for elusive bacteria and paves the way for large-scale studies of more complex animal microbial communities.

show abstract

Specific Strains of Honeybee Gut Lactobacillus Stimulate Host Immune System to Protect against Pathogenic Hafnia alvei

Abstract: Honeybees are essential pollinators supporting global agricultural economies and food supplies. Recent honeybee decline has been linked to several factors, while pathogen infection is considered one of the most significant contributing factors.

Cited by 58 publications

References 62 publications

The Impact of Environmental Habitats and Diets on the Gut Microbiota Diversity of True Bugs (Hemiptera: Heteroptera)

The Impact of Environmental Habitats and Diets on the Gut Microbiota Diversity of True Bugs (Hemiptera: Heteroptera)

Bumblebees with the socially transmitted microbiome: A novel model organism for gut microbiota research

Strain-level profiling with picodroplet microfluidic cultivation reveals host-specific adaption of honeybee gut symbionts

Contact Info

Product

Resources

About