Specific suppression of mixed lymphocyte reactions by alloactivated cells is correlated with cytotoxicity

Vandekerckhove, Bart; Jong, E. Timmermans‐De; Termijtelen, A.; Claas, F. H. J.

doi:10.1016/0198-8859(89)90059-1

Cited by 4 publications

(10 citation statements)

References 15 publications

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“…Radioactivity was counted in a Packard Multi Prias Gcounter. The percentage of specific *'Cr release was calculated as described [21].…”

Section: Generation Of the Secondary Mlr Culture (Smc)mentioning

confidence: 99%

“…Then, 1 fid of ['H]thymidine was added followed by an additional incubation for 16-20 h. Subsequently, the cultures were harvested onto glass-fibre filters and [^H]thymidine incorporation was determined by liquid scintillation spectroscopy. The percentage of specific suppression was calculated as described [21]. When suppression was titrated, the S50 value was used [21], which is defined as the minimal number of cells in thousands necessary to suppress a given MLR by 50%.…”

Section: Generation Of the Secondary Mlr Culture (Smc)mentioning

confidence: 99%

“…Based on these findings we argued that cytotoxic T lymphocytes (CTL) might be the cells responsible for the observed suppression, probably by killing the antigen-presenting cells of the stimulator (or the responding T-cell blasts in the reverse MLR; see Ref. 21).…”

mentioning

confidence: 98%

“…In vitro alloactivated lymphocytes can display potent suppressive activity when added to a mixed lymphocyte reaction (MLR) [8,9]. This suppression is specific for the HLA class I and/or class II antigens expressed on the original stimulator cell [ 13,15,20,21 ]. Recently, we were able to demonstrate a positive \mear correlation between suppression of a panel of mixed lymphocyte reactions and cytolytic activity against each stimulator cell.…”

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confidence: 99%

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Cytotoxic T Lymphocytes are the Prime Mediators of Suppression of the Mixed Lymphocyte Reaction by Alloactivated Cells

et al. 1989

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An alloactivated secondary mixed lymphocyte reaction (MLR) culture (SMC), which was suppressive in the MLR with autologous responder cells, was studied in more detail. In particular, we investigated whether the suppressive activity of this SMC was mediated by cytotoxic T cells or whether bona fide suppressor cells were involved. The SMC suppressed an MLR when the stimulator cells shared Bw57, Bw60, or Dw19 with the original stimulator. Separation of the SMC into CD4+ and CD8+ fractions demonstrated that the CD8+ fraction contained suppressive and cytotoxic activity against Bw57 or Bw60 antigens, while the CD4+ fraction contained both activities against the Dw19 specificity. The CD8+ fraction was also suppressive in the reverse MLR, while the CD4+ fraction was not. Limiting dilution analysis demonstrated that all CD8+-suppressive cultures were also cytotoxic, whereas in the CD4+ fraction both cytotoxic and non-cytotoxic suppressor cultures were found.

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“…Radioactivity was counted in a Packard Multi Prias Gcounter. The percentage of specific *'Cr release was calculated as described [21].…”

Section: Generation Of the Secondary Mlr Culture (Smc)mentioning

confidence: 99%

Section: Generation Of the Secondary Mlr Culture (Smc)mentioning

confidence: 99%

mentioning

confidence: 98%

mentioning

confidence: 99%

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Cytotoxic T Lymphocytes are the Prime Mediators of Suppression of the Mixed Lymphocyte Reaction by Alloactivated Cells

et al. 1989

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“…including tumour immunopathoiogy [1]. The mixed lymphocyte culture (MLC) interaction remains the most widely applicable in vitro model for alloreactivity in which parameters for the generation of suppressor T-Lph can be investigated [2][3][4][5][6][7][8][9][10]. For example, non-cylotoxic alloanti gen-specific CD8 + suppressor T-cell clones have been generated in this system [11].…”

mentioning

confidence: 99%

Different Conditions for Generation of Non‐Cytotoxic Autorestricted CD4^‐ CD8⁺ Human Suppressor T Cells and Allospecific Cytotoxic T Lymphocytes in One‐Way Mixed Lymphocyte Cultures: the Role of Adherent Mononuclear Cells

Balashov¹,

Brondz²

1991

Scand J Immunol

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The purpose of the present study was to investigate the role of adherent mononuclear cells (AMC) in generation of suppressor T lymphocytes (T-Lph) in one-way allogeneic mixed lymphocyte cultures (MLC). Alloantigen-specific cytotoxic T-Lph were generated in MLC predominantly in the presence of stimulator AMC, but not responder AMC. The presence of responder AMC and the absence of stimulator AMC were optimal conditions for generation of non-cytotoxic (through the whole duration of MLC) suppressor T-Lph. These CD4-CD8+ suppressor T-Lph were allononspecific but autorestricted (with responders in test MLC).

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γδ T-cell receptor-positive T-cell clones derived from human heart transplants do not show donor-specific cytotoxicity

et al. 1990

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Specific suppression of mixed lymphocyte reactions by alloactivated cells is correlated with cytotoxicity

Cited by 4 publications

References 15 publications

Cytotoxic T Lymphocytes are the Prime Mediators of Suppression of the Mixed Lymphocyte Reaction by Alloactivated Cells

Cytotoxic T Lymphocytes are the Prime Mediators of Suppression of the Mixed Lymphocyte Reaction by Alloactivated Cells

Different Conditions for Generation of Non‐Cytotoxic Autorestricted CD4^‐ CD8⁺ Human Suppressor T Cells and Allospecific Cytotoxic T Lymphocytes in One‐Way Mixed Lymphocyte Cultures: the Role of Adherent Mononuclear Cells

γδ T-cell receptor-positive T-cell clones derived from human heart transplants do not show donor-specific cytotoxicity

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Specific suppression of mixed lymphocyte reactions by alloactivated cells is correlated with cytotoxicity

Cited by 4 publications

References 15 publications

Cytotoxic T Lymphocytes are the Prime Mediators of Suppression of the Mixed Lymphocyte Reaction by Alloactivated Cells

Cytotoxic T Lymphocytes are the Prime Mediators of Suppression of the Mixed Lymphocyte Reaction by Alloactivated Cells

Different Conditions for Generation of Non‐Cytotoxic Autorestricted CD4‐ CD8+ Human Suppressor T Cells and Allospecific Cytotoxic T Lymphocytes in One‐Way Mixed Lymphocyte Cultures: the Role of Adherent Mononuclear Cells

γδ T-cell receptor-positive T-cell clones derived from human heart transplants do not show donor-specific cytotoxicity

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Different Conditions for Generation of Non‐Cytotoxic Autorestricted CD4^‐ CD8⁺ Human Suppressor T Cells and Allospecific Cytotoxic T Lymphocytes in One‐Way Mixed Lymphocyte Cultures: the Role of Adherent Mononuclear Cells