Specific survivin dual fluorescence resonance energy transfer molecular beacons for detection of human bladder cancer cells

Wang, Zhiqiang; Zhao, Jun; Zeng, Jin; Wu, Kaijie; Chen, Yule; Wang, Xin-Yang; Chang, Luke S.; He, Dalin

doi:10.1038/aps.2011.122

Cited by 7 publications

(5 citation statements)

References 20 publications

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“…An example of dual fluorescence imaging is when surviving dual fluorescence resonance energy transfers molecular beacons with fluorophores to form one donor–acceptor pair. This occurs in order to improve the specific detection of cancer cells [ 37 ]. In this study, the 400-nm pitch of a plasmonic chip exhibited an advantage for the dual-color fluorescence microscopic observation.…”

Section: Resultsmentioning

confidence: 99%

Dual-Color Fluorescence Imaging of EpCAM and EGFR in Breast Cancer Cells with a Bull’s Eye-Type Plasmonic Chip

Izumi

Yamamura

Hayashi

et al. 2017

Sensors

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Surface plasmon field-enhanced fluorescence microscopic observation of a live breast cancer cell was performed with a plasmonic chip. Two cell lines, MDA-MB-231 and Michigan Cancer Foundation-7 (MCF-7), were selected as breast cancer cells, with two kinds of membrane protein, epithelial cell adhesion molecule (EpCAM) and epidermal growth factor receptor (EGFR), observed in both cells. The membrane proteins are surface markers used to differentiate and classify breast cancer cells. EGFR and EpCAM were detected with Alexa Fluor® 488-labeled anti-EGFR antibody (488-EGFR) and allophycocyanin (APC)-labeled anti-EpCAM antibody (APC-EpCAM), respectively. In MDA-MB231 cells, three-fold plus or minus one and seven-fold plus or minus two brighter fluorescence of 488-EGFR were observed on the 480-nm pitch and the 400-nm pitch compared with that on a glass slide. Results show the 400-nm pitch is useful. Dual-color fluorescence of 488-EGFR and APC-EpCAM in MDA-MB231 was clearly observed with seven-fold plus or minus two and nine-fold plus or minus three, respectively, on the 400-nm pitch pattern of a plasmonic chip. Therefore, the 400-nm pitch contributed to the dual-color fluorescence enhancement for these wavelengths. An optimal grating pitch of a plasmonic chip improved a fluorescence image of membrane proteins with the help of the surface plasmon-enhanced field.

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Section: Resultsmentioning

confidence: 99%

Dual-Color Fluorescence Imaging of EpCAM and EGFR in Breast Cancer Cells with a Bull’s Eye-Type Plasmonic Chip

Izumi

Yamamura

Hayashi

et al. 2017

Sensors

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“…Wang et al [24], in their study used confocal microscopy to prove that the fluorescent signal was higher in patients with BC than in healthy patients. The authors recommended the usage of this method for early detection of recurrence.…”

Section: Discussionmentioning

confidence: 99%

“…In our study, overexpression of BRIC5 was discovered in urine samples from patients with BC and compared to BRIC5 level in healthy people and revealed a statistically significant difference. Wang et al [ 24 ], in their study used confocal microscopy to prove that the fluorescent signal was higher in patients with BC than in healthy patients. The authors recommended the usage of this method for early detection of recurrence.…”

Section: Discussionmentioning

confidence: 99%

BASIC SCIENCE HIF-1, GLUT1, endoglin, and BIRC5 expression in urine samples obtained from patients with bladder malignancies – after photodynamic diagnosis (PDD)

et al. 2012

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IntroductionBladder cancer (BC) is a serious medical problem. The high rate of recurrence and progression demands the development of new methods, such as genetic markers, which allow diagnosis and patient follow-up.ObjectivesThe aim of this study was to compare expression of HIF-1, GLUT1, endoglin, and BRIC5 in patients without and those with BC. The second group was divided into sub-groups: those without a history of PDD (photodynamic diagnosis) in the diagnostic process and those after PDD.MethodsPatients with BC were diagnosed using the PDD method using hexaminolevulinate (Hexvix®). The expressions of HIF-1, GLUT1, endoglin, and BRIC5 genes were established in urine specimens by real-time quantitative polymerase chain reaction (PCR).ResultsThe expressions of all tested genes were higher in the group of patients with BC than in the group without BC. In the group after PDD, a statistically significant overexpression of HIF-1 was observed. In this group, changes were not observed in cases of the other three tested genes.ConclusionsThe differences between the group with PDD and the group without it can be connected with the direct influence of PDD on malignant tissue, which can cause overexpression of HIF-1 only. This is, however, only a hypothesis and needs further study.

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“…One approach measures miRNA with fluorescein (FAM) or cyanine dye 3 (Cy3) fluorophores in tumour cell lines [13], which enables a more accurate observation of miRNA by fluorescence microscopy. The interaction of miRNAs and other molecules can be studied based by the FRET principle.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA Quantitation During Dendritic Cell Endocytosis Using Imaging Flow Cytometry: Key Factors and Requirements

Tong

Zhu

Zhang

et al. 2018

Cell Physiol Biochem

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Background/Aims: MicroRNA (miRNA)-induced suppression of dendritic cells (DCs) has been implicated in many diseases. Therefore, accurate monitoring of miRNA endocytosis by DCs is important for understanding the role of miRNAs in many diseases. Recently, a method for measuring the co-localization of Argonaute 2 (AGO2)-associated miRNAs on laser-scanning confocal microscopy method was proposed to localize the miRNAs. But its definition was limited by the number of observed cells through its accuracy. Methods: In this study, a method based on imaging flow cytometry was developed to localize miR-590-5p with fluorescent probes in DCs. miR-590-5p proven to play an important role in tumor immunity. This method enabled the quantification, visualization and localization of the fluorescence intensity in 30,000 individual cells. Results: Using this method, the DCs with different endocytotic ability were distinguished. The behaviour of miR-590-5p during endocytosis under the stimulation of tumor antigen in DCs was observed, binding to its cognate target mRNA and degradation in DCs. Conclusion: This method based on imaging flow cytometry provide an additional method to study miRNA processing in DCs, which makes it a valuable addition to existing miRNA research techniques.

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Specific survivin dual fluorescence resonance energy transfer molecular beacons for detection of human bladder cancer cells

Cited by 7 publications

References 20 publications

Dual-Color Fluorescence Imaging of EpCAM and EGFR in Breast Cancer Cells with a Bull’s Eye-Type Plasmonic Chip

Dual-Color Fluorescence Imaging of EpCAM and EGFR in Breast Cancer Cells with a Bull’s Eye-Type Plasmonic Chip

BASIC SCIENCE HIF-1, GLUT1, endoglin, and BIRC5 expression in urine samples obtained from patients with bladder malignancies – after photodynamic diagnosis (PDD)

MicroRNA Quantitation During Dendritic Cell Endocytosis Using Imaging Flow Cytometry: Key Factors and Requirements

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