Specification of auditory sensitivity by Drosophila TRP channels

Göpfert, Martin C.; Albert, Joerg T.; Nadrowski, Björn; Kamikouchi, Azusa

doi:10.1038/nn1735

Cited by 157 publications

(232 citation statements)

References 15 publications

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“…Mechanical amplification and spontaneous mechanical emissions have also been demonstrated in the hearing organ of Drosophila (Göpfert et al, 2005(Göpfert et al, , 2006. Functional mechanotransduction was required for both phenomena, indicating that as postulated in vertebrates, the transduction machinery in Drosophila is necessary for producing active amplification (Göpfert et al, 2006).…”

Section: Introductionmentioning

confidence: 82%

“…Functional mechanotransduction was required for both phenomena, indicating that as postulated in vertebrates, the transduction machinery in Drosophila is necessary for producing active amplification (Göpfert et al, 2006). Adaptation has also been characterized in Drosophila (Walker et al, 2000), making the flies well suited for studying the contribution of both active amplification and adaptation to gain control in mechanotransduction.…”

Section: Introductionmentioning

confidence: 93%

“…Measurements of antennal motions and compound action potentials indicate that the auditory system of the fly actively amplifies mechanical stimuli (Göpfert et al, 2006) and adapts to them (Albert et al, 2007). Such studies are well suited for defining the contribution of molecular components to amplification processes, because of the sensitive measurements of mechanical motions (Albert et al, 2007).…”

Section: Gain Control Mechanisms In Drosophila Mechanotransductionmentioning

confidence: 99%

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Dissection of Gain Control Mechanisms inDrosophilaMechanotransduction

Chadha

Cook

2012

J. Neurosci.

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Mechanoreceptor cells respond to a vast span of stimulus intensities, which they transduce into a limited response-range using a dynamic regulation of transduction gain. Weak stimuli are detected by enhancing the gain of responses through the process of active mechanical amplification. To preserve responsiveness, the gain of responses to prolonged activation is rapidly reduced through the process of adaptation. We investigated long-term processes of mechanotransduction gain control by studying responses from single mechanoreceptor neurons in Drosophila. We found that mechanical stimuli elicited a sustained reduction of gain that we termed long-term adaptation. Long-term adaptation and the adaptive decay of responses during stimuli had distinct kinetics and they were independently affected by manipulations of mechanotransduction. Therefore, long-term adaptation is not associated with the reduction of response gain during stimulation. Instead, the long-term adaptation suppressed canonical features of active amplification which were the high gain of weak stimuli and the spontaneous emission of noise. In addition, depressing amplification using energy deprivation recapitulated the effects of long-term adaptation. These data suggest that long-term adaptation is mediated by suppression of active amplification. Finally, the extent of long-term adaptation matched with cytoplasmic Ca 2ϩ levels and dTrpA1-induced Ca 2ϩ elevation elicited the effects of long-term adaptation. Our data suggest that mechanotransduction employs parallel adaptive mechanisms: while a rapid process exerts immediate gain reduction, long-term adjustments are achieved by attenuating active amplification. The slow adjustment of gain, manifest as diminished sensitivity, is associated with the accumulation of Ca 2ϩ .

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Section: Introductionmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 93%

Section: Gain Control Mechanisms In Drosophila Mechanotransductionmentioning

confidence: 99%

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Dissection of Gain Control Mechanisms inDrosophilaMechanotransduction

Chadha

Cook

2012

J. Neurosci.

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“…1A). Sound-induced antennal rotations are also smaller in nompC mutants at some of these particle velocities, due to a loss of active amplification [3], so we controlled for this by measuring sound-evoked antennal movements in wild type flies and mutants, and plotting the sound response data relative to antennal rotations.…”

Section: Loss Of Nompc Decreases the Sensitivity Of Generator Currentmentioning

confidence: 99%

“…NompC or TRPN1 was proposed as a candidate member of the transduction complex that converts mechanical force into an electrical signal in Drosophila auditory receptor neurons, in part due to its requirement for active amplification of sound-induced antennal motion [2,3,8,11,13]. NompC is present in arthropod, fish, and amphibian genomes but absent from those of birds and mammals.…”

Section: Introductionmentioning

confidence: 99%

Separate TRP channels mediate amplification and transduction in drosophila

Lehnert¹,

Baker²,

Wilson³

2015

AIP Conference Proceedings

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Abstract. Auditory receptor cells rely on mechanically-gated channels to transform sound stimuli into neural activity. Several TRP channels have been implicated in Drosophila auditory transduction, but mechanistic studies have been hampered by the inability to record subthreshold signals from receptor neurons. We developed a non-invasive method for measuring these signals by recording from a central neuron that is electrically coupled to a genetically-defined population of auditory receptors. We find that the TRPN family member NompC, which is necessary for the active amplification of motion by the auditory organ, is not required for transduction. Instead, NompC sensitizes the transduction complex to movement and precisely regulates the static forces on the complex. In contrast, the TRPV channels Nanchung and Inactive are required for responses to sound, suggesting they are components of the transduction complex. Thus, transduction and active amplification are genetically separable processes in Drosophila hearing.

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Conservation of Pain Genes Across Evolution

Khuong

Neely

2013

Pain Genetics

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Specification of auditory sensitivity by Drosophila TRP channels

Cited by 157 publications

References 15 publications

Dissection of Gain Control Mechanisms inDrosophilaMechanotransduction

Dissection of Gain Control Mechanisms inDrosophilaMechanotransduction

Separate TRP channels mediate amplification and transduction in drosophila

Conservation of Pain Genes Across Evolution

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