Specificities and genetic characteristics of nucleosome-reactive antibodies from autoimmune mice

Monestier, Marc; Novick, Kristine E.

doi:10.1016/0161-5890(95)00115-8

Cited by 54 publications

(35 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ELISA data 11,13,14,17,18 demonstrate that the chromatin epitope recognized by PL2-6 is a conformational one, involving a ternary complex of H2A, H2B and DNA. Even though the epitope is present in the binary complex of H2A and H2B, reactivity is augmented by complexing with (mixed sequence) DNA on a surface, as in ELISA plates, or in solution.…”

Section: Discussionmentioning

confidence: 99%

“…Most of our current knowledge about the binding specificity of the epichromatin antibody (PL2-6) is derived from ELISA studies. 11,13,14,17,18 We know, based upon ELISA quantitation, that PL2-6 binds strongly to mononucleosomes and to a ternary complex of histones H2A + H2B + DNA, weakly to H2A + H2B and very weakly to H3 + H4 + DNA, individual histones or DNA alone. In the present immunoblotting results with both native Hela and recombinant H2A and H2B.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

An epichromatin epitope: Persistence in the cell cycle and conservation in evolution

Olins¹,

Langhans²,

Monestier³

et al. 2011

nucleus

Self Cite

View full text Add to dashboard Cite

NucleusVolume 2 Issue 1 U2OS and HL-60/S4 cells by deconvolution immunofluorescence microscopy with the aim of searching for chromatin substructures. One anti-nucleosome antibody (PL2-6) specifically stained a chromatin compartment at the periphery of interphase nuclei, as well as staining the surface of mitotic chromosomes. We suggest that this compartment represents a unique surface chromatin conformation, to which we assign the name "epichromatin". Furthermore, we formulate an "epichromatin hypothesis", suggesting that this chromatin may play a crucial role in organizing interphase nuclear architecture, justifying its conservation in the evolution of cell structure. Results

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An epichromatin epitope: Persistence in the cell cycle and conservation in evolution

Olins¹,

Langhans²,

Monestier³

et al. 2011

nucleus

Self Cite

View full text Add to dashboard Cite

show abstract

“…Because nucleosomes are a common autoantigen in both murine and human systemic autoimmune disease (42,43), initial studies were conducted with several MRL-lpr-derived IgG2a anti-nucleosome mAbs (31,34). Nucleosomes were purified from bovine thymus as described previously (39).…”

Section: Rf ϩ B Cells Can Be Stimulated Directly By Monoclonal Anti-nmentioning

confidence: 99%

“…The nucleosome-specific mAbs PR1-3, PL2-6, PL2-3, LG4-1, MRB-4, LG10-1, LG8-1, PL9-7, PL2-8, PL2-7, and MGC 23 were derived from MRL mice and purified by protein G affinity chromatography (31)(32)(33)(34) …”

Section: Serum Samples and Absmentioning

confidence: 99%

Immune Complexes Present in the Sera of Autoimmune Mice Activate Rheumatoid Factor B Cells

Rifkin

Leadbetter²,

Beaudette³

et al. 2000

The Journal of Immunology

Self Cite

View full text Add to dashboard Cite

The fate of an autoreactive B cell is determined in part by the nature of the interaction of the B cell receptor with its autoantigen. In the lpr model of systemic autoimmunity, as well as in certain human diseases, autoreactive B cells expressing rheumatoid factor (RF) binding activity are prominent. A murine B cell transgenic model in which the B cell receptor is a RF that recognizes IgG2a of the j allotype (IgG2aj), but not the b allotype, was used in this study to investigate how the form of the autoantigen influences its ability to activate B cells. We found that sera from autoimmune mice, but not from nonautoimmune mice, were able to induce the proliferation of these RF+ B cells but did not stimulate B cells from RF− littermate controls. The stimulatory factor in serum was found to be IgG2aj, but the IgG2aj was stimulatory only when in the form of immune complexes. Monomeric IgG2aj failed to stimulate. Immune complexes containing lupus-associated nuclear and cytoplasmic autoantigens were particularly potent B cell activators in this system. Appropriate manipulation of such autoantibody/autoantigen complexes may eventually provide a means for therapeutic intervention in patients with certain systemic autoimmune disorders.

show abstract

“…cell antigen in murine (18,19,30,(51)(52)(53) and human (26,54,55) lupus-like syndromes. Alternatively, these antibodies may have no genealogical relationship and arose independently.…”

Section: Discussionmentioning

confidence: 99%

Autoimmunity caused by disruption of central T cell tolerance. A murine model of drug-induced lupus.

Kretz-Rommel¹,

Duncan²,

Rubin³

1997

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

Specificities and genetic characteristics of nucleosome-reactive antibodies from autoimmune mice

Cited by 54 publications

References 43 publications

An epichromatin epitope: Persistence in the cell cycle and conservation in evolution

An epichromatin epitope: Persistence in the cell cycle and conservation in evolution

Immune Complexes Present in the Sera of Autoimmune Mice Activate Rheumatoid Factor B Cells

Autoimmunity caused by disruption of central T cell tolerance. A murine model of drug-induced lupus.

Contact Info

Product

Resources

About