Specificities of the Skin Morphology in Juvenile Minipigs

Gauthier, B.; Penard, Laure; Bordier, Nicole; Briffaux, Jean-Paul; Ruty, Bernard

doi:10.1177/0192623318804520

Cited by 8 publications

(10 citation statements)

References 11 publications

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“…The value of the pig model has been shown in many studies [5][6][7][8][9]. Due to multiple similarities in the structure and physiology of human and pig skin, this model is very useful for subcutaneous (SC) formulations testing [10]. Moreover, the Fc receptor of pigs, critical for the elimination half-life of therapeutic monoclonal antibodies (mAb), is similar to the Fc human receptor [11].…”

Section: Introductionmentioning

confidence: 99%

WITHDRAWN: Ustekinumab pharmacokinetics after subcutaneous administration in pig

Grabowski¹,

Burmańczuk²,

Derlacz³

et al. 2019

Preprint

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Background. Due to multiple similarities in the structure and physiology of human and pig skin, the pig model is very useful for biological drugs testing after subcutaneous administration. The differences between subcutaneous injection sites could have significant impact on the absorption phase and pharmacokinetic profiles of biologic drugs. The aim of the current study was to analyse pharmacokinetics and selected biochemical and hematological parameters after a single subcutaneous administration of ustekinumab – human therapeutic monoclonal antibody, in regular pigs and compare parameters following administration into external or internal regions of the inguinal fold. The subjects were 12 healthy female large white piglets. Each group received a single dose of ustekinumab given in a 1 mg/kg subcutaneous injection into the internal inguinal fold or external part of the inguinal fold. Results. An analysis of hematological parameters showed that a single subcutaneous dose of ustekinumab at dose 1 mg/kg of body weight in pigs does not cause changes in the hematological parameters that go beyond the physiological limits. The differences in absorption rate between the internal inguinal fold or external part of the inguinal fold were not significant. However the time of maximal concentration, clearance, area under the curve calculated between zero and mean residence time and mean residence time between groups were substantially different (p>0.05). The relative bioavailability after external part of the inguinal fold administration was 40.36% lower than after internal inguinal fold ustekinumab administration. Conclusions. Breeding pig has been shown to be a relevant pharmacokinetics model to study subcutaneously administered ustekinumab.

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Section: Introductionmentioning

confidence: 99%

WITHDRAWN: Ustekinumab pharmacokinetics after subcutaneous administration in pig

Grabowski¹,

Burmańczuk²,

Derlacz³

et al. 2019

Preprint

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“…The minipig is considered an animal model of choice for skin studies, as this model shares many common features with human skin, including low hair density, epidermal morphology and thickness. [13,23] The morphological, biochemical and biomechanical features of porcine skin that mimic human skin have been established in previous studies via in vitro methods. [15,[33][34][35][36] However, optical coherence tomography (OCT), [35,37] high-frequency ultrasound (HFUS), [38] photoacoustic imaging (PAI), [39] MPM [26,27] and reflectance confocal microscopy (RCM) [40] have demonstrated their capability for acquiring skin images in vivo.…”

Section: Discussionmentioning

confidence: 99%

“…[9] Among different animal models of skin, the pig or minipig is often the species of choice in dermal studies based on the anatomical, physiological, and immunological similarities with that of human skin. [10][11][12][13] Similar to human skin, porcine skin has well developed rete ridges and dermal papilla, similar dermal-epidermal thickness ratio, epidermal turnover time, vascular anatomy and collagen structure. [14][15][16] Additionally, porcine skin is tightly attached to subcutaneous connective tissue as is human skin.…”

Section: Introductionmentioning

confidence: 99%

“…Skin from several animal species including mice, rats, pigs and monkeys are typically used for pre‐clinical studies in dermatology [9] . Among different animal models of skin, the pig or minipig is often the species of choice in dermal studies based on the anatomical, physiological, and immunological similarities with that of human skin [10‐13] . Similar to human skin, porcine skin has well developed rete ridges and dermal papilla, similar dermal‐epidermal thickness ratio, epidermal turnover time, vascular anatomy and collagen structure [14‐16] .…”

Section: Introductionmentioning

confidence: 99%

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In vivo characterization of minipig skin as a model for dermatological research using multiphoton microscopy

Alex

Chaney

Žurauskas

et al. 2020

Experimental Dermatology

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Minipig skin is one of the most widely used non‐rodent animal skin models for dermatological research. A thorough characterization of minipig skin is essential for gaining deeper understanding of its structural and functional similarities with human skin. In this study, three‐dimensional (3‐D) in vivo images of minipig skin was obtained non‐invasively using a multimodal optical imaging system capable of acquiring two‐photon excited fluorescence (TPEF) and fluorescence lifetime imaging microscopy (FLIM) images simultaneously. The images of the structural features of different layers of the minipig skin were qualitatively and quantitatively compared with those of human skin. Label‐free imaging of skin was possible due to the endogenous fluorescence and optical properties of various components in the skin such as keratin, nicotinamide adenine dinucleotide phosphate (NAD(P)H), melanin, elastin and collagen. This study demonstrates the capability of optical biopsy techniques, such as TPEF and FLIM, for in vivo non‐invasive characterization of cellular and functional features of minipig skin, and the optical image‐based similarities of this commonly utilized model of human skin. These optical imaging techniques have the potential to become promising tools in dermatological research for developing a better understanding of animal skin models, and for aiding in translational pre‐clinical to clinical studies.

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“…[96][97][98][99][100] The minipig epidermal thickness varies only minimally between birth and adulthood, whereas the dermal and especially the subcutaneous thicknesses increases over time. 101 Although the epidermis generally consists of 5 to 7 cell layers with only minor regional variations, the thickness of porcine epidermis varies from 30 to 140 mm (compared to 50-120 mm in human skin) and is thinner in the abdominal region and thicker at the neck, particularly in males. Similarly, the dermis and subcutis are thicker on the dorsum and thinner at the abdomen and inner thighs.…”

Section: Chapter 8 Integumentary Systemmentioning

confidence: 99%

International Harmonization of Nomenclature and Diagnostic Criteria (INHAND): Nonproliferative and Proliferative Lesions of the Minipig

et al. 2021

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The INHAND (International Harmonization of Nomenclature and Diagnostic Criteria for Lesions) Project ( www.toxpath.org/inhand.asp ) is a joint initiative of the Societies of Toxicologic Pathology from Europe (ESTP), Great Britain (BSTP), Japan (JSTP), and North America (STP) to develop an internationally accepted nomenclature for proliferative and nonproliferative lesions in laboratory animals. The purpose of this publication is to provide a standardized nomenclature for classifying microscopic lesions observed in most tissues and organs from the minipig used in nonclinical safety studies. Some of the lesions are illustrated by color photomicrographs. The standardized nomenclature presented in this document is also available electronically on the internet ( http://www.goreni.org/ ). Sources of material included histopathology databases from government, academia, and industrial laboratories throughout the world. Content includes spontaneous lesions as well as lesions induced by exposure to test materials. Relevant infectious and parasitic lesions are included as well. A widely accepted and utilized international harmonization of nomenclature for lesions in laboratory animals will provide a common language among regulatory and scientific research organizations in different countries and increase and enrich international exchanges of information among toxicologists and pathologists.

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Specificities of the Skin Morphology in Juvenile Minipigs

Cited by 8 publications

References 11 publications

WITHDRAWN: Ustekinumab pharmacokinetics after subcutaneous administration in pig

WITHDRAWN: Ustekinumab pharmacokinetics after subcutaneous administration in pig

In vivo characterization of minipig skin as a model for dermatological research using multiphoton microscopy

International Harmonization of Nomenclature and Diagnostic Criteria (INHAND): Nonproliferative and Proliferative Lesions of the Minipig

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