Specificity of 
            5l
            Cr-Release Cytotoxicity of Lymphocytes Immune to Murine Sarcoma Virus
            2

Herberman, Ronald B.; Aoki, Tadao; Nunn, Myrthel E.; Lavrin, David H.; Soares, Neci Matos; Gazdar, Adi F.; Holden, Howard T.; Chang, Kenneth S. S.

doi:10.1093/jnci/53.4.1103

Cited by 136 publications

(45 citation statements)

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“…All of the surface antigenic markers studied here have been previously found to be associated with expression of murine type C viruses [5,9]. It is not clear, however, whether each of these antigens is characteristic of a the cell lines described above (same antigenic pattern as S16CI-10-I) particular virus or can be induced by a variety of exogenous or endogenous type C viruses.…”

Section: Discussionmentioning

confidence: 79%

“…Two different absorptions of test antisera, in vivo and in vitro, were used as described by Aoki et al [5], Ferritin-conjugated IgG purified from appropriate heteroantisera were ab sorbed in vitro with target cells as described elsewhere [5], Celt-mediated cytotoxicity and inhibition tests. These techniques were used for the demon stration of MEV-SA1 [9], Briefly, C57BL/6 mice were inoculated intramuscularly with Moloney murine sarcoma virus (M-MSV), and spleen cells harvested 13-16 days after virus inoculation were used as attacking cells. Ten million attacking cells were incubated with 5 x io4 51Cr-labeled C57BL/6 Rauscher leukemia RBL-5 target cells for 4 h at 37° [12].…”

Section: Antigen Systems Tested See Table IImentioning

confidence: 99%

“…The mechanisms of these changes in host range have been extensively studied from the genetic, virologic, and molecular biologic standpoints [3]. An immunologic approach to this problem can also be very useful, since viral envelope antigens (VEAs) and cell surface antigens (CSAs) associated with endogenous type C viruses have been analyzed to a large extent by various immunologic techniques including immunoelectron microscopy (IEM) [4][5][6], neutralization tests [7,8], and in vitro cell-mediated immune response [9]. These studies defined several VEAs and CSAs as summarized in table II.…”

mentioning

confidence: 99%

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Heterogeneity of Surface Antigens on Endogenous Type C Virus-Producing Cell Sublines Derived from a Clonal Line of BALB/3T3 Cells

Aoki¹,

Herberman²,

Liu³

1975

Intervirology

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When viral envelope antigens (VEAs) and virus-associated cell surface antigens (CSAs) are used as markers of type C viruses, more heterogeneity of virus populations can be demonstrated than by using host range alone. In the present study, four CSAs (PCI, X.l, GCSA, and MEV-SA1) and three VEAs (xVEA, xl-VEA, and sub-gsVEA) were used as markers of virus populations present in various sublines of the BALB/c embryonic fibroblast BALB/3T3 clone A31 line. Of four spontaneously transformed sublines, two released N-tropic endogenous type C viruses spontaneously after long-term culture and each had distinct antigenic patterns. Treatment with 5-bromodeoxyuridine (BrdU) resulted in the detection of X-tropic viruses in all four sublines. The expression of these X-tropic viruses was associated with two different antigenic patterns. When the clonal rabbit corneal SIRC cell line was infected with the X-tropic viruses obtained after BrdU treatment, the cells acquired detectable amounts of only one of the antigenic markers.

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Section: Discussionmentioning

confidence: 79%

Section: Antigen Systems Tested See Table IImentioning

confidence: 99%

mentioning

confidence: 99%

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Heterogeneity of Surface Antigens on Endogenous Type C Virus-Producing Cell Sublines Derived from a Clonal Line of BALB/3T3 Cells

Aoki¹,

Herberman²,

Liu³

1975

Intervirology

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“…(See also Hofer & Hughes, 1970. ) Cytotoxicity assay The activity of natural killer (NK) cells was determined in a 51Cr release assay, as previously described (Herberman et al, 1974) (Belardelli et al, 1984). Tumour titration carried out in DBA/2 hosts confirms that the same differences can be observed after i.v.…”

Section: Irradiationmentioning

confidence: 92%

Natural resistance in mice against Friend cells injected intravenously. III. Comparison between in vivo and in vitro passaged interferon-sensitive (745) and interferon-resistant (3Cl8) cell clones

Neri

Zei²,

Bonmassar³

et al. 1989

Br J Cancer

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Summary In vitro (FLC-Vt) or in vivo (FLC-V) passaged Friend erythroleukaemia cells of DBA/2 origin were tested for susceptibility to natural resistance (NR) in vivo or to NK cell activity in vitro. Scarcely oncogenic FLC-Vt cells were highly susceptible to in vivo NR (measured as rapid organ clearance or growth inhibition in lethally irradiated mice) or to in vitro NK attack. Conversely, highly oncogenic FLC-V cells were weakly susceptible to NR and to NK as well. These data seem to point out that natural immunity, which is up-regulated by endogenous or exogenous interferons, can play a significant role in surveillance against mouse leukaemic cells of retrovirus origin.It has been reported that the in vivo tumour suppressive effects of interferon (IFN) against in vitro and in vivo passaged Friend erythroleukaemia cells (FLC-Vt and FLC-V, respectively), derived from DBA/2 mice infected with Friend virus, might involve activation of immune host's mechanism rather than acting directly on tumour cells (Belardelli et al., 1982a;Gresser et al., 1983Gresser et al., , 1988). This hypothesis was based on the isolation of FLC clones differing for sensitivity to IFN-alpha-beta-induced antiproliferative effects under in vitro conditions (Affabris et al., 1982). Using the highly tumorigenic IFN-sensitive (745) or IFN-resistant (3C18) FLC-V clones it was demonstrated that exogenous IFN preparations could suppress FLC-V in vivo growth, irrespective of the differences in IFN sensitivity (Belardelli et al., 1982a;Gresser et al., 1988). Moreover, it was observed that the low oncogenicity of both 745-and 3C18-FLC-Vt clones was due to host's reactivity enhanced by endogenous IFN, since pretreatment of mice with anti-IFN antibodies augmented FLC-Vt tumorigenicity (Gresser et al., 1983).Previous investigations were carried out to detect the possible origin of host's immune reactivity involved in the indirect antitumour effects of IFN. The results showed that in vivo natural resistance (NR) against both FLC-Vt (Iorio et al., 1986) and FLC-V clones could play a significant role in this system. The present study shows that the low oncogenic FLC-Vt clone is subjected to strong NR in syngeneic DBA/2 mice and is more susceptible to NR effectors as compared with the highly oncogenic FLC-V. Irradiation Animals were subjected to total body irradiation using a cobalt-60 irradiator (Hot Spot MKIV, Harwell, England), delivering gamma-rays at the rate of 800Rmin-m.Organ clearance of 125I-iodedeoxyuridine (12 5IUdR)-labelled cells The general procedure for this assay has been previously described (Riccardi et al., 1980). Briefly 25 x 106 FLC-V or FLC-Vt in 50ml of RPMI 1640 medium supplemented with 10% fetal calf serum were incubated overnight at 37°C in a

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“…Although the existence of a lymphocyte population which inhibits tumour growth in vivo has not yet been reported (Ilfeld et al, 1973;Nordlund and Gershon, 1975), in vitro evidence suggests the presence of cytotoxic T cells and cytostatic B cells in the M-MuSV system (Hellstrom and Hellstr6m, 1969;Herberman et al, 1974;Lamon et al, 1972;Plata et al, 1974;Leclerc et al, 1972;Lamon et al, 1974;Plata et al, 1976) and the regression of tumours induced by regressor M-MuSV has been attributed to the immune response of the host animals (Fefer et al, 1968;Weinert et al, 1974). In our experiments with progressor M-MuSV, the regressive stage and the first plateau stage observed in adult mice, as well as the occasional regression of tumours, may also be ascribed to the results of a host immune response.…”

Section: (I) Non-specific Tumour Enhancementmentioning

confidence: 99%

Enhancing and inhibiting effects of spleen cells from tumour-bearing mice on growth of virus-induced primary sarcoma

Kimura¹,

Aoki²

1978

Br J Cancer

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Summary.-The effects of adoptive transfer of spleen cells from tumour-bearing mice on the growth of Moloney murine sarcoma virus (M-MuSV)-induced primary tumours in BALB/c mice were studied. The effects were in 2 directions, depending on the time of lymphocyte inoculation; when spleen cells from tumour-bearing mice 12 days after M-MuSV inoculation were inoculated into recipient mice before M-MuSV inoculation, the appearance of tumours was significantly delayed and their incidence was reduced, whereas when these lymphocytes were inoculated after the development of tumours in recipient mice, tumour growth was significantly enhanced. The data indicated that these inhibiting and enhancing effects were mediated mainly by T cells. The mechanisms of the paradoxical effects were investigated.

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Specificity of 5l Cr-Release Cytotoxicity of Lymphocytes Immune to Murine Sarcoma Virus 2

Cited by 136 publications

References 0 publications

Heterogeneity of Surface Antigens on Endogenous Type C Virus-Producing Cell Sublines Derived from a Clonal Line of BALB/3T3 Cells

Heterogeneity of Surface Antigens on Endogenous Type C Virus-Producing Cell Sublines Derived from a Clonal Line of BALB/3T3 Cells

Natural resistance in mice against Friend cells injected intravenously. III. Comparison between in vivo and in vitro passaged interferon-sensitive (745) and interferon-resistant (3Cl8) cell clones

Enhancing and inhibiting effects of spleen cells from tumour-bearing mice on growth of virus-induced primary sarcoma

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