Specificity of endothelial cell reorientation in response to cyclic mechanical stretching

Wang, James H.‐C.; Goldschmidt‐Clermont, Pascal J.; Wille, Jeremiah J.; Yin, F. C. P.

doi:10.1016/s0021-9290(01)00150-6

Cited by 306 publications

(355 citation statements)

References 20 publications

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“…The same principle applies to individual cells for determining cell deformations (Lee et al, 1996). Because of variation in the adhesion strength of individual cells to underlying substrate and incomplete strain transmission from substrate to cells, the strains experienced by individual cells vary and are generally smaller than substrate surface strains (Wang et al, 2001).…”

Section: Fibroblasts and Mechanical Loadsmentioning

confidence: 99%

Mechanoregulation of gene expression in fibroblasts

Wang

Thampatty

Lin

et al. 2007

Gene

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227

187

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Mechanical loads placed on connective tissues alter gene expression in fibroblasts through mechanotransduction mechanisms by which cells convert mechanical signals into cellular biological events, such as gene expression of extracellular matrix components (e.g., collagen). This mechanical regulation of ECM gene expression affords maintenance of connective tissue homeostasis. However, mechanical loads can also interfere with homeostatic cellular gene expression and consequently cause the pathogenesis of connective tissue diseases such as tendinopathy and osteoarthritis. Therefore, the regulation of gene expression by mechanical loads is closely related to connective tissue physiology and pathology. This article reviews the effects of various mechanical loading conditions on gene regulation in fibroblasts and discusses several mechanotransduction mechanisms. Future research directions in mechanoregulation of gene expression are also suggested.

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Section: Fibroblasts and Mechanical Loadsmentioning

confidence: 99%

Mechanoregulation of gene expression in fibroblasts

Wang

Thampatty

Lin

et al. 2007

Gene

Self Cite

227

187

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“…Through these links, the role of the cytoskeleton has been shown to regulate many processes in mechanical stimulation including the remodeling of the cytoskeleton under fluid shear stress, the strengthening of mechanical response through connecting intermediate filaments to intercellular adhesion sites, and remodeling the actin cytoskeleton in the direction of deformation. 12,43,113 Furthermore, studies of stress and strain on cells have increased the understanding of mechanics with respect to intracellular behavior involving the cytoskeleton. 35,39 From a biochemical perspective, local modulation of integrin receptors affects cell behavior because applying mechanical stresses alters the expression of cyclic AMP as well as induces local recruitment of mRNA and ribosomes to focal adhesions.…”

Section: Mechanotransductionmentioning

confidence: 99%

“…Over the past several decades, microtechnology such as micropipette aspiration devices has improved the understanding of membrane mechanics including: the interconnection between the membrane of the outer hair cell with its cortical lattice; the cell-cycle-regulated viscoelastic properties of hepatocellular carcinoma cells; and the volume differential behavior of red blood cells under an anisotonic environment, which has direct and indirect links to the cytoskeleton. 19,71,113 Fluid shearing devices have helped uncover protein-specific attachment sites and the contributions of the cytoskeleton to them. 17,49,61,87,106,110 Alternately, other methods can examine local domains.…”

Section: Nano-and Microtechnology To Address the Cytoskeletonmentioning

confidence: 99%

“…21,23,32,45,72,74,99 The cytoskeleton itself has been shown to contribute directly to many processes in mechanical stimulation including its own remodeling under fluid shear stress, the strengthening of mechanical response by connecting the intermediate filaments to intercellular adhesion sites, and remodeling the actin cytoskeleton in the direction of minimal deformation under equibiaxial and uniaxial strain with elastomeric membranes. 12,43,113 . Moreover, forces distributed through the cytoskeleton have been shown to have long-range effects propagating into the nucleus, where single chromosomes can be displaced through mechanical manipulations of cells at their surface.…”

Section: Introductionmentioning

confidence: 99%

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Nanoscale Intracellular Organization and Functional Architecture Mediating Cellular Behavior

LeDuc

Bellin

2006

Ann Biomed Eng

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Abstract-Cells function based on a complex set of interactions that control pathways resulting in ultimate cell fates including proliferation, differentiation, and apoptosis. The interworkings of this immensely dense network of intracellular molecules are influenced by more than random protein and nucleic acid distribution where their interactions culminate in distinct cellular function. By probing the design of these biological systems from an engineering perspective, researchers can gain great insight that will aid in building and utilizing systems that are on this size scale where traditional large-scale rules may fail to apply. The organized interaction and gradient distribution in intracellular space imply a structural architecture that modulates cellular processes by influencing biochemical interactions including transport and binding-reactions. One significant structure that plays a role in this modulation is the cell cytoskeleton. Here, we discuss the cytoskeleton as a central and integrating functional structure in influencing cell processes and we describe technology useful for probing this structure. We explain the nanometer scale science of cytoskeletal structure with respect to intracellular organization, mechanotransduction, cytoskeletal-associated proteins, and motor molecules, as well as nano-and microtechnologies that are applicable for experimental studies of the cytoskeleton. This biological architecture of the cytoskeleton influences molecular, cellular, and physiological processes through structured multimodular and hierarchical principles centered on these functional filaments. Through investigating these organic systems that have evolved over billions of years, understanding in biology, engineering, and nanometer-scaled science will be advanced.

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“…For instance, cells orient perpendicular to cyclic strain when cultured on stiff substrates or on biaxially constrained collagen gels (1)(2)(3)(4)(5)(6)(7). This phenomenon, called strain avoidance (SA), depends on the profile of the cyclic strain because the degree of cellular (re)orientation is proportional to the amplitude and frequency of the strain (1,2).…”

Section: Introductionmentioning

confidence: 99%

Prediction of Cell Alignment on Cyclically Strained Grooved Substrates

Ristori

Vigliotti

Baaijens

et al. 2016

Biophysical Journal

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Cells respond to both mechanical and topographical stimuli by reorienting and reorganizing their cytoskeleton. Under certain conditions, such as for cells on cyclically stretched grooved substrates, the effects of these stimuli can be antagonistic. The biophysical processes that lead to the cellular reorientation resulting from such a competition are not clear yet. In this study, we hypothesized that mechanical cues and the diffusion of the intracellular signal produced by focal adhesions are determinants of the final cellular alignment. This hypothesis was investigated by means of a computational model, with the aim to simulate the (re)orientation of cells cultured on cyclically stretched grooved substrates. The computational results qualitatively agree with previous experimental studies, thereby supporting our hypothesis. Furthermore, cellular behavior resulting from experimental conditions different from the ones reported in the literature was simulated, which can contribute to the development of new experimental designs.

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Specificity of endothelial cell reorientation in response to cyclic mechanical stretching

Cited by 306 publications

References 20 publications

Mechanoregulation of gene expression in fibroblasts

Mechanoregulation of gene expression in fibroblasts

Nanoscale Intracellular Organization and Functional Architecture Mediating Cellular Behavior

Prediction of Cell Alignment on Cyclically Strained Grooved Substrates

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