Specificity of helper T-cells generated from macaques infected with attenuated simian immunodeficiency virus.

Dittmer, Ulf; Feldmann, G; Sauermann, Ulrike; Spirng, M; Überla, Klaus; Stahl–Hennig∥, Christiane; Hunsmann, Gerhard

doi:10.1099/0022-1317-79-7-1801

Cited by 5 publications

(2 citation statements)

References 21 publications

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“…Nonetheless, studies have suggested that HIV-specific CD4 cells are important in the control of viremia in patients infected with HIV (Rosenberg et al 1997;Pitcher et al 1999;Kaufmann et al 2004;. As an extension, several groups have identified SIV-specific CD4 cells in various non-human primate models of SIV or SHIV infection (Mills et al 1991;Lekutis and Letvin 1997;Dittmer et al 1998;Kuroda et al 2000;Sarkar et al 2002). Within these analyses, one group examined MHC class II DRB restriction of the HIV envelope in a SHIV model (Lekutis and Letvin 1997;Kuroda et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Comprehensive characterization of MHC class II haplotypes in Mauritian cynomolgus macaques

et al. 2007

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There are currently no non-human primate models with fully defined major histocompatibility complex (MHC) class II genetics. We recently showed that 6 common MHC haplotypes account for essentially all MHC diversity in cynomolgus macaques (Macaca fascicularis) from the island of Mauritius. Here we employ cDNA cloning and sequencing to comprehensively characterize full length MHC class II alleles expressed at the Mafa-DPA, -DPB, -DQA, -DQB, -DRA, and -DRB loci on the 6 common haplotypes. We describe 34 full-length MHC class II alleles, 12 of which are completely novel. Polymorphism was evident at all six loci, including DPA which is considered monomorphic in rhesus macaques. Similar to other old world monkeys, Mauritian cynomolgus macaques (MCM) share MHC class II allelic lineages with humans at the DQ and DR loci, but not at the DP loci. Additionally, we identified extensive sharing of MHC class II alleles between MCM and other non-human primates. The characterization of these full length expressed MHC class II alleles will enable researchers to generate MHC class II transferent cell lines and tetramers that can be used to explore CD4+ T-lymphocyte responses in MCM.

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Section: Discussionmentioning

confidence: 99%

Comprehensive characterization of MHC class II haplotypes in Mauritian cynomolgus macaques

et al. 2007

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“…Dittmer et al (46) described a comprehensive analysis of Env SU-specific Th responses by using cultured short-term T cell lines from four monkeys inoculated with an attenuated SIV, while excluding comparative analysis of fine Th responses elicited to other SIV proteins. Thus, the current comparative analysis of the broadly reactive Th epitope peptides from SU and Gag showed that, despite similarity in protein lengths, SU contained only ϳ15% of the mapped broadly reactive Th epitope peptides, while Gag contained 40% of the epitopes.…”

Section: Discussionmentioning

confidence: 99%

Detailed Analysis of CD4+ Th Responses to Envelope and Gag Proteins of Simian Immunodeficiency Virus Reveals an Exclusion of Broadly Reactive Th Epitopes from the Glycosylated Regions of Envelope

Sarkar

Kalia

Murphey‐Corb

et al. 2002

The Journal of Immunology

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Ag-specific CD4+ Th cells play a key role in the development, maturation, and maintenance of pathogen-specific humoral and cellular immune responses. To define the fine specificity of broadly reactive Th responses associated with mature immunity in a lentiviral system, we analyzed peptide-specific Th responses in eight macaques chronically infected with a reference live attenuated SIV at 12–14 mo postinoculation. All macaques had stable immunocompetent Th cells at the time of analysis, and a unique array of Th responses to 20-mer overlapping peptides from envelope (Env) and Gag was identified for each macaque, which were then used to define a set of 31 broadly reactive peptide epitopes. Only 5 of the 31 broadly reactive Th epitope peptides mapped to the surface (SU) domain of Env. Interestingly, these were all confined to two conserved nonglycosylated regions toward the carboxyl terminus of SU, suggesting a structural influence of glycosylation on development of Th responses. Gag and the Env transmembrane proteins contained the majority of broadly reactive peptide epitopes (12 and 14 peptides, respectively), which were uniformly distributed throughout their sequence. This study defines for the first time broadly reactive Th epitope peptides of SIV Env and Gag proteins that are associated with enduring broadly protective vaccine immunity to attenuated SIV, which may be used for the design and evaluation of experimental vaccines. Moreover, the data suggest that extensive glycosylation of SU may provide yet another immune escape mechanism developed by lentiviruses to restrict the breadth of Th repertoire to SU, a major immunologically exposed protein of the virus.

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Lebendimpfstoffe gegen HIV: Chancen und Risiken

Dittmer¹

2000

HIV-Infekt

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Specificity of helper T-cells generated from macaques infected with attenuated simian immunodeficiency virus.

Cited by 5 publications

References 21 publications

Comprehensive characterization of MHC class II haplotypes in Mauritian cynomolgus macaques

Comprehensive characterization of MHC class II haplotypes in Mauritian cynomolgus macaques

Detailed Analysis of CD4+ Th Responses to Envelope and Gag Proteins of Simian Immunodeficiency Virus Reveals an Exclusion of Broadly Reactive Th Epitopes from the Glycosylated Regions of Envelope

Lebendimpfstoffe gegen HIV: Chancen und Risiken

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