Specificity of IgG subclass antibodies in different clinical manifestations of leprosy

Abstract

SUMMARYWe analysed specific IgG subclasses levels to Mycobacterium leprae sonieale cxiraci (MSE), lipoarabinomannan B (LAM) and phenolic glycolipid I (PGL-l)in the sera of leprosy patients with different elinieal manifestations. IgG2 was found to be the predominant antibody to MSE regardless of clinical manifestations, and IgG I response was mostly seen in lepromatous patients, IgG3 reacted only rarely but IgG4 reacted relatively more in certain elinieal groups such as borderline lepromalous and lepromatous with erythema nodosum leprosum (ENL) reaction. Mosl of the IgG subclass responses to MSE could be accounted for reactivity with LAM, suggesting that LAM is the major immunogen involved in the pathogenesis of leprosy. In contrast to LAM. PGL-I antigen showed considerably lower reactivities for IgG subelasses. An association between IgG subclass responses and clinical manifestations of leprosy was also seen. Whereas borderline lepromatous patients were found to have significantly higher leveis of lgG2 and IgG4 to MSE. lepromatous patients had elevated levels of IgGl and lower levels of IgG2. An interesting observation, however, was the significantly higher levels of IgG2 to LAM in the pure neuritic leprosy patients.

Comparative Evaluation of Profiles of Antibodies to Mycobacterial Capsular Polysaccharides in Tuberculosis Patients and Controls Stratified by HIV Status

“…However, studies have documented the heterogeneity of Ab responses to proteins in HIV Ϫ TB cases, which might be explained by the broad range of clinical presentations at the time of sample acquisition (9,21,24,34,35). Despite a predominant IgG2 response, variations in IgG subclass titers to LAM were associated with different clinical manifestations of leprosy (11). It is conceivable that several factors in our HIV Ϫ TB patients contributed to the lack of significant correlation between AM and either MS or MPT51: (i) the stronger IgG responses to AM than to MS and MPT51, (ii) the differences in IgG subclass responses, and (iii) the heterogeneity of IgG responses to mycobacterial proteins.…”

Features of intrathecal immunoglobulins in patients with multiple sclerosis

“…Since each isotype and subclass of Ig's has distinct biological functions, the preponderance of a specific subclass could be crucial for the outcome of a disease [13,14]. However, in MS correlations of immunoglobulins and autoantigenic profiles with disease evolution and severity are still under debate.…”

Biotechnological and Immunological Platforms Based on PGL-I Carbohydrate-Like Peptide of Mycobacterium leprae for Antibodies Detection Among Leprosy Clinical Forms

“…Regarding the IgG subclasses in leprosy, although controversial, it has been previously demonstrated that IgG3 antibody levels were higher in lepromatous than in tuberculoid patients (Beuria et al, 1998). Similarly, it has also been shown that IgG1 was mostly found in LL patients (Dhandayuthapani et al, 1992), whereas IgG2 and IgG4 presented higher levels in TT patients (Hussain et al, 2004). However, it is claimed that IgG2 antibodies are the predominant subclass across the disease spectrum (Beuria et al, 1998).…”