Specificity of monoclonal antibodies obtained by immunization of mice with trypsin-treated group a streptococcus culture

Abstract

Hybridomas producing monoclonal antibodies intensively reacting with group A streptococcus antigens in enzyme immunoassay were obtained as a result of immunizing mice with pepsin-treated cultures of group A streptococcus. All antibodies were referred to class M immunoglobulins. The reactions of monoclonal antibodies were completely inhibited by the pepsin-treated culture of group A streptococcus. The degree of inhibition with A-polysaccharide was lower, being 17.5 to 50.0 in different monoclonal antibodies. All the monoclonal antibodies obtained cross-reacted with antigens of murine and human epithelial tissues of the thymus and skin.

Counts of Stromal Precursor Cells in Heterotopic Bone Marrow Transplants in Mice Immunized with Group A Streptococcus Antigens

“…It was previously shown that these antibodies are directed to organ-specific myocardial antigens common for animals of different species (rabbit, mouse, ox, etc. ), that is, they were autoantibodies [1,3]. Hence, immunization of animals according to the above protocol results in production of not only antibodies to streptococcal antigens, but also autoantibodies.…”

“…The mice were intraperitoneally immunized with the vaccine in ascending doses during 3 weeks (3 consecutive days in each week): 1 billion bacterial cells/injection on week 1, 2 billions on week 3, and 3 billions on week 3. Immunization of animals (rabbits, mice) according to this protocol leads to the formation (in addition to antibodies to streptococcal antigens) of autoantibodies to various tissue antigens, the maximum antibody production was observed on days 7-14 after the last injection of the antigens [1,3]. Autoantibodies to heart tissue antigens were detected in the sera of immune mice by indirect immunofluorescence on bovine heart tissue sections.…”

“…After immunization of animals (rabbits, mice) with cultures of some streptococcus types (1, 5, 12, et al), containing cross-reacting antigens, autoantibodies reacting with normal tissue components were formed in the sera of these animals [1,5]. Hybrydomas producing monoclonal antibodies cross-reacting with human and animal tissue antigens were obtained by immunization of mice by group A streptococcal cultures [3]. Another aspect in this problem is the use of stromal tissue transplantation for the treatment of some pathologies [8].…”

Preparation and characterization of monoclonal antibodies to group-specific antigenic determinant of group a streptococcus polysaccharide

“…The results of these and previous [5] studies indicate that A-PS contains at least 4 cross-reacting determinants. The dominant role of rhamnose in this epitopes suggests the presence of cross-reacting determinants common for skin epithelial and thymic antigens in group A streptococci and in other microorganisms, whose polysaccharide contains rhamnose.…”

“…Challenge dose (3X10 9 bacterial cells) was injected intravenously on day 25 after the last injection to mice with high anti-A-PS antibody titer. After 2 days splenocytes were fused with murine plasmacytoma cells [5]. Antibodies to A-PS in the sera, supernatants, and aseitic fluids containing MAb were detected by ELISA [7], or agar gel immunodiffusion [2].…”