Specificity of oligonucleotide probes complementary to evolutionarily variable regions of 16S rRNA from Mycoplasma hyopneumoniae and Mycoplasma hyorhinis

Johansson, Karl‐Erik; Mattsson, Jens G.; Jacobsson, Karin; Fernández, Carlos M. Fernández; Bergström, Katrin; Bölske, Göran; Wallgren, Per; Göbel, Ulf B.

doi:10.1016/0034-5288(92)90010-y

Cited by 15 publications

(9 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, the nonpathogenic Mycoplasma flocculare is commonly found in swine lungs and has many morphologic, growth, and antigenic characteristics in common with M. hyopneumoniae (3,18). These similarities are also supported by molecular data (10,21,23).…”

supporting

confidence: 72%

“…Samples from experimentally infected pigs. Bronchial lavage samples and ground lung tissue samples from experimentally infected pigs were prepared as described earlier (10). Prior to the PCR, 100-l samples were washed in PBS, resuspended in water, and lysed by boiling as described above.…”

Section: Methodsmentioning

confidence: 99%

“…However, none of these systems were used to analyze clinical samples. We have earlier reported about an oligonucleotide probe targeting 16S rRNA suitable for direct detection of M. hyopneumoniae in lung biopsy specimens from pigs suffering from pneumonia in the acute phase (10). In the present work we describe a PCR system based on amplification of the 16S rRNA gene for the detection of M. hyopneumoniae and the testing of this PCR on nasal swabs taken from conventional pigs in a fattening herd.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Detection of Mycoplasma hyopneumoniae in nose swabs from pigs by in vitro amplification of the 16S rRNA gene

et al. 1995

View full text Add to dashboard Cite

We report the use of PCR to detect DNA from Mycoplasma hyopneumoniae, the etiological agent of enzootic porcine pneumonia. A primer set was designed for the amplification of a 649-bp fragment of the 16S rRNA gene from M. hyopneumoniae. The PCR product was identified by ethidium bromide staining after gel electrophoresis and by Southern hybridization with an M. hyopneumoniae-specific oligonucleotide probe. No amplification was observed from any other porcine bacteria tested, including several closely related mycoplasmas. It was also possible to demonstrate the presence of M. hyopneumoniae in bronchial lavage samples and lung tissue samples from experimentally infected pigs. Furthermore, the PCR system was used for analysis of nasal samples obtained from pigs in a fattening herd. By this method, we were able to detect M. hyopneumoniae in nose swabs from naturally infected pigs. However, our results suggest that M. hyopneumoniae can be detected in the nasal cavities only during a limited time period.

show abstract

supporting

confidence: 72%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Detection of Mycoplasma hyopneumoniae in nose swabs from pigs by in vitro amplification of the 16S rRNA gene

et al. 1995

View full text Add to dashboard Cite

show abstract

“…Recently, DNA recombinant techniques have been directly applied to diagnosis and vaccine production. Synthetic oligonucleotide probes have been developed to detect a variety of infectious agents, for example, enteroviruses, rhinoviruses or mycoplasmas (Bruce et aL, 1989a,b;Johansson et al, 1992). Recombinant virus vaccines have been produced to control a number of infectious diseases, for example rabies (Wiktor et aL, 1988).…”

Section: Introductionmentioning

confidence: 99%

Application of the polymerase chain reaction (PCR) in veterinary diagnostic virology

Belák

Ballagi-Pordány

1993

Vet Res Commun

View full text Add to dashboard Cite

show abstract

“…hyopneumoniae genome have been developed as diagnostic tools for identification purposes by DNA hybridization (1,100,217). DNA probes labeled with "P, or '^'l have been used to detect 10 pg of mycoplasma DNA (equivalent to 10* organisms) (217).…”

Section: Mycoplasma Hyopneumoniaementioning

confidence: 99%

Sequence and functional analysis of the P97 swine cilium adhesin gene of Mycoplasma hyopneumoniae

Hsu

View full text Add to dashboard Cite

Specificity of oligonucleotide probes complementary to evolutionarily variable regions of 16S rRNA from Mycoplasma hyopneumoniae and Mycoplasma hyorhinis

Cited by 15 publications

References 36 publications

Detection of Mycoplasma hyopneumoniae in nose swabs from pigs by in vitro amplification of the 16S rRNA gene

Detection of Mycoplasma hyopneumoniae in nose swabs from pigs by in vitro amplification of the 16S rRNA gene

Application of the polymerase chain reaction (PCR) in veterinary diagnostic virology

Sequence and functional analysis of the P97 swine cilium adhesin gene of Mycoplasma hyopneumoniae

Contact Info

Product

Resources

About