Specificity of the juvenile hormone binding protein: The geometrical isomers of juvenile hormone I

Goodman, Walter G.; Schooley, David A.; Gilbert, Lawrence I.

doi:10.1073/pnas.75.1.185

Cited by 53 publications

(30 citation statements)

References 12 publications

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“…Twenty-hydroxyecdysone (20E) (a gift from Dr. Takeshi Matsumoto, Daicel Chemical Co.) was dissolved in ethanol and stored at À20°C. The concentrations of JH I, methoprene and 20E were determined spectrophotometrically [e 217 = 14,800 in MeOH for JH I, Goodman et al (1978); e 261 = 28,000 in hexane for methoprene, Osir and Riddiford (1988); e 240 = 12,677 in EtOH for 20E, Lafont and Wilson (1992)]. For the JH experiments in vitro, pieces of integument were pre-cultured for 2 h with respective concentrations for the actual culture, then transferred to the culture medium containing JH I or JH I and 20E.…”

Section: Tissue Culture and Hormonesmentioning

confidence: 99%

The role of 20-hydroxyecdysone and juvenile hormone in pupal commitment of the epidermis of the silkworm, Bombyx mori

Muramatsu

Kinjoh

Shinoda

et al. 2008

Mechanisms of Development

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During the pupal metamorphosis in insects, cellular commitment for pupal differentiation must precede before its differentiation. The pupal commitment of Bombyx mori epidermis occurred from day 3 to day 6 last (5th) instar larvae in response to the gradual increase in ecdysteroid titer in the presence of a small amount of juvenile hormone (JH). Yet the concealed preparatory process of the commitment had begun in the newly synthesized 5th instar larval epidermis (approximately 6 h before the ecdysis) as a competence phase, in which pupal commitment in vitro was induced by 20-hydroxyecdysone (20E) but inhibited by JH. This competence phase continued until day 2 5th instar, and the decrease and increase in cellular sensitivity to JH and 20E, respectively, occurred gradually during this period. In early day 3, autonomous pupal commitment began in vitro and 20E stimulated the commitment, but JH could only partially prevent the commitment in both cases. This apparent reversible to irreversible transition ended in early day 6 by the completion of pupal commitment, when the cells completely lost their sensitivity to JH and no longer expressed the larval cuticle protein gene 30. The expression of the transcription factor, broad, closely followed the commitment, so that we could use this gene expression as a molecular marker for pupal commitment. These results indicate that exposure to 20E and loss of the sensitivity of the epidermal cells to JH are required for the completion of pupal commitment, and suggest that the unusually long process over 3 days could be due to the presence of the detectable JH during the commitment.

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Section: Tissue Culture and Hormonesmentioning

confidence: 99%

The role of 20-hydroxyecdysone and juvenile hormone in pupal commitment of the epidermis of the silkworm, Bombyx mori

Muramatsu

Kinjoh

Shinoda

et al. 2008

Mechanisms of Development

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show abstract

“…The hormone was quantified spectrophotometrically (Goodman et al, 1978) using a spectrophotometer calibrated according to the method of Schmid (1990). The purified hormone was dissolved in acetone just before application and placed in a dispensing chamber to prevent inadvertent concentration of the hormone during application (Goodman and Allington, 1990).…”

Section: Hormone Treatmentmentioning

confidence: 99%

Embryonic expression of juvenile hormone binding protein and its relationship to the toxic effects of juvenile hormone in Manduca sexta

Orth

Tauchman

Doll

et al. 2003

Insect Biochemistry and Molecular Biology

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“…3 LMW juvenile hormone binding proteins (JHBPs) demonstrate stereoselectivity and high affinity towards JH molecules (K d below 10 − 6 M) and almost no binding of JH degradation products (JH diol and JH acid). [11][12][13][14] Among the JHBPs, the protein from Galleria mellonella, the object of the present study, is of particular importance as the insect, commonly known as greater wax moth, is perceived as the worst beehive pest in the world. 15 Devastation of honeycombs results from the nutritional habits of the larvae, which ingest honey, pollen and wax.…”

Section: Introductionmentioning

confidence: 99%

Insect Juvenile Hormone Binding Protein Shows Ancestral Fold Present in Human Lipid-Binding Proteins

Kolodziejczyk

Bujacz

Jakób

et al. 2008

Journal of Molecular Biology

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Specificity of the juvenile hormone binding protein: The geometrical isomers of juvenile hormone I

Abstract: The binding of the geometrical isomers (>99%

Cited by 53 publications

References 12 publications

The role of 20-hydroxyecdysone and juvenile hormone in pupal commitment of the epidermis of the silkworm, Bombyx mori

The role of 20-hydroxyecdysone and juvenile hormone in pupal commitment of the epidermis of the silkworm, Bombyx mori

Embryonic expression of juvenile hormone binding protein and its relationship to the toxic effects of juvenile hormone in Manduca sexta

Insect Juvenile Hormone Binding Protein Shows Ancestral Fold Present in Human Lipid-Binding Proteins

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