Specificity protein-1 and -3 trans-activate the ovine placental lactogen gene promoter

Jeckel, Kimberly M.; Limesand, Sean W.; Anthony, Russell V.

doi:10.1016/j.mce.2009.04.006

Cited by 5 publications

(3 citation statements)

References 29 publications

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“…CSH1 expression is well correlated to the maturation of BNCs among bovine trophoblasts (Nakano et al 2005); however, information on the regulation and transcription factors for CSH1 is still limited. Some elements, AP2, GATAs, PURA, and SPs, have been reported as activation factors for this gene, and CSH1 expression was closely related to the implantation process spatiotemporally (Limesand et al 2004, Jeckel et al 2009). GATA may play a regulatory role of CSH1 expression and differentiation of BNCs (Bai et al 2011).…”

Section: Discussionmentioning

confidence: 98%

Bone morphogenetic protein 4 accelerates the establishment of bovine trophoblastic cell lines

Suzuki¹,

Koshi²,

Imai³

et al. 2011

Reproduction

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Trophoblastic cells play a crucial role in implantation and placentogenesis. A large proportion of the failures of conception in cows occur in the peri-implantation period, which are known as early embryo losses. In exploring this critical phenomenon, trophoblastic cell lines can provide substantial information. Unfortunately, there are few cell lines for this purpose in cattle because of the difficulty of raising successive cell stock in the long term. In this study, 12 new cell lines were established using bone morphogenetic protein 4 (BMP4). BMP4 stimulated embryonic cells to enter the trophoblastic cell lineage but there were no significant differences between intact and BMP4-treated groups. Only one out of 49 embryos developed trophoblastic cells in the intact group. Finally, 12 cell lines were maintained for around 30 passages, and they retained trophoblastic characteristics and expressed bovine trophoblastic genes: placental lactogen, interferon-t, pregnancy-associated glycoprotein 1, and prolactin-related protein 1. Although the gene expression patterns were different among cell lines and depended on the cells, there was no significant relationship between the expression intensities of genes and the treatment dose of BMP4. All of them expressed bovine POU domain class 5 transcription factor 1 and caudal-type homeobox 2. The expression of these genes was confirmed by quantitative RT-PCR and immunohistochemical detection. These results suggest that BMP4 is involved in the raising of trophoblast cell lines from early embryonic cells and the newly developed cell lines can provide different types of bovine trophoblastic cells with different cell lineages. This may constitute a significant new tool for the examination of trophoblastic differentiation.

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Section: Discussionmentioning

confidence: 98%

Bone morphogenetic protein 4 accelerates the establishment of bovine trophoblastic cell lines

Suzuki¹,

Koshi²,

Imai³

et al. 2011

Reproduction

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“…For cold competition (CC), 200-fold molar excess of unlabeled homologous oligonucleotides were added to EMSA binding reactions. As described earlier [ 63 ], for super-shift assays, 1 μl of normal rabbit serum or HIF-1α antiserum (Active Motif) was pre-incubated with NE in the 1x binding buffer for 2 hours at 4°C. This was followed by addition of biotinylated probe and poly (dI-dC).…”

Section: Methodsmentioning

confidence: 99%

SIRT1 increases cardiomyocyte binucleation in the heart development

et al. 2018

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SIRT1 regulates cell senescence. We investigated a novel role of SIRT1 in the regulation of cardiomyocyte terminal differentiation in the developing heart. Retinoic acid (RA)-induced binucleation of H9c2 cells was associated with increased SIRT1 expression. Inhibition of SIRT1 activity or expression significantly decreased RA-induced binucleation. SIRT1 expression was minimal in the fetal heart and significantly upregulated in the hearts of postnatal day 7 (P7) rat pups. In contrast, heart-specific miR-133a expression was high in the fetal heart but significantly reduced in P7 pup hearts. The miR-133a promoter contains a canonical HRE element and hypoxia upregulated miR-133a gene expression in the heart. SIRT1 mRNA 3′UTR has miR-133a binding sequences and miR-133a and hypoxia suppressed SIRT1 expression in cardiomyocytes. Of importance, inhibition of SIRT1 significantly reduced binucleated cardiomyocytes in the hearts of P7 pups. Taken together, the present study reveals a novel role of SIRT1 and its regulation by miR-133a in cardiomyocyte terminal differentiation of the developing heart, and suggests a potential therapeutic strategy that may impact cardiac function later in life.

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“…The positive effect of leptin on CSH2 expression also may encourage the continued use of CT1 cells to study CSH2 gene expression. Previous examinations of bovine or ovine CSH2 expression regulation required heterologous transfection studies using human and rodent choriocarcinoma lines [18,19]. Marginal CSH2 expression occurs in bovine trophoblast cell lines, including CT1 cells under normal growth conditions.…”

Section: Discussionmentioning

confidence: 99%

Activities for leptin in bovine trophoblast cells

Hughes

Xie

McCoski

et al. 2017

Domestic Animal Endocrinology

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Leptin is involved in various reproductive processes in humans and rodents, including placental development and function. The specific ways that leptin influences placental development and function in cattle are poorly understood. This work was completed to explore how leptin regulates hormone, cytokine and metalloprotease transcript abundance, and cell proliferation in cultured bovine trophoblast cells. In the first set of studies, cells were cultured in the presence of graded recombinant bovine leptin concentrations (0, 10, 50, 250 ng/mL) for 6 or 24 h. Transcript profiles were examined from extracted RNA. Leptin supplementation did not affect abundance of the maternal recognition of pregnancy factor, interferon-tau (IFNT), but leptin increased (P < 0.05) abundance of chorionic somatomammotropin hormone 2 (CSH2; ie, placental lactogen) at both 6 and 24 h at each concentration tested. At 24 h, the greatest CSH2 abundance (P < 0.05) was detected in cells supplemented with 50 ng/mL leptin. Transcript abundance of the remodeling factor, metalloprotease 2 (MMP2), was greater (P < 0.05) in leptin-treated cells at 24 h but not at 6 h. The 24 h MMP2 response was greatest (P < 0.05) at 250 ng/mL. Transcript abundance for MMP9 was not altered by leptin treatment. In a separate set of studies, cell proliferation assays were completed. Leptin supplementation did not affect bovine trophoblast cell line proliferation at any dose tested. In conclusion, leptin supplementation did not affect bovine trophoblast cell proliferation or IFNT expression, but leptin increases CSH2 and MMP2 transcript abundance. Both of these factors are involved with peri-implantation and postimplantation placental development and function, and this implicates leptin as a potential mediator of early placental development and function in cattle.

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Specificity protein-1 and -3 trans-activate the ovine placental lactogen gene promoter

Cited by 5 publications

References 29 publications

Bone morphogenetic protein 4 accelerates the establishment of bovine trophoblastic cell lines

Bone morphogenetic protein 4 accelerates the establishment of bovine trophoblastic cell lines

SIRT1 increases cardiomyocyte binucleation in the heart development

Activities for leptin in bovine trophoblast cells

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