Speckle tracking echocardiography in the diagnosis of early left ventricular systolic dysfunction in type II diabetic mice

Abstract

AbstractsBackgroundThe leptin receptor-deficient db/db mouse is a well-established type II diabetes animal model used to investigate diabetic cardiomyopathy. Previous reports have documented diabetic cardiomyopathy is accompanied by cardiac structural and functional abnormalities. To better elucidate early or subtle changes in cardiac performance in db/db mice, we used speckle tracking echocardiography to assess systolic myocardial strain in vivo with diabetic db/db mice in order to study early changes of left ventricle contractile function in type II diabetes model.MethodsMale diabetic db/db mice and age-matched control mice from C57BL/6J strain at 8,12 and 16 weeks of age were subjected to echocardiography. At the midpapillary level in the parasternal left ventricular short-axis view, end diastolic and systolic left ventricular diameter, interventricular septal thickness and posterior wall thicknesses, ejection fraction, fractional shortening were determined by M-mode echocardiography. Using speckle-tracking based strain analysis of two-dimensional echocardiographic images acquired from the parasternal short-axis views at the mid-papillary level, systolic global radial and circumferential strain values were analyzed.ResultsThere was no significant difference in interventricular septal thickness, posterior wall thicknesses, end diastolic and systolic left ventricular diameter, ejection fraction and fractional shortening between db/db and age-matched control mice at 8,12 or 16 weeks of age (P > 0.05). At 8 and 12 weeks of age, there was no significant difference in left ventricular radial strain and circumferential strain between db/db mice and age-matched controls (P > 0.05). But at 16 weeks of age, the left ventricular radial strain and circumferential strain in db/db mice were lower than in control mice (P < 0.01).ConclusionThe present study shows that speckle tracking echocardiography can be used to evaluate cardiac functional alterations in mouse models of cardiovascular disease. Radial and circumferential strain are more sensitive and can be used for detection of early left ventricular contractile dysfunction in db/db type II diabetic mice.

The additional utility of two‐dimensional strain in detection of coronary artery disease presence and localization in patients undergoing dobutamine stress echocardiogram

“…3 Two-dimensional strain has been recently presented as a strong predictor of different cardiac pathologies including CAD. 4,5 Since the use of ultrasound strain in identifying the acutely ischemic myocardium was reported in 2003 6 the role of two-dimensional strain in the diagnoses of CAD at rest has been investigated in a number of studies involving different patients' groups like diabetic patients or those with suspected new onset non-ST segment elevation acute coronary syndrome. 7,8 It was not until recently that the incremental value of twodimensional strain to DSE has been investigated.…”

Advances in Echocardiographic Imaging in Heart Failure With Reduced and Preserved Ejection Fraction

“…For example, the association between early myocardial dysfunction and abnormal interstitial depositions of collagen and amyloid material has been elucidated in several animal models of HFpEF. 47,48 Similarly, in a rat model of chronic hypertension, it was recently noticed that progressive disruption of T-tubule and defects in calcium cycling started at an early stage and were associated with progressive subclinical reductions in systolic and diastolic myocardial mechanics. These changes preceded the occurrence of clinical symptoms and development of cardiac fibrosis, which was associated with the fall in LVEF.…”

Cardiometabolic Traits and Systolic Mechanics in the Community

“…36 We now extend prior findings to also highlight a role for leptin in relation to LV systolic mechanics in humans, which corroborates prior findings in leptin-receptor deficient animal models. 37 It is important to note that data linking circulating leptin concentrations to clinical outcomes in population-based studies have shown conflicting results, with some studies suggesting higher risk and others a null or even protective effect. 38-40 …”