Spectral analysis for diagnosis of esophagus dysplasia using fluorescence Raman spectroscopy

Abstract

In this paper, we present a three parametric model using Raman and fluorescence spectroscopy for detection and analysis of esophagus dysphasia and esophagus cancer. The model was set up from more than 200 samples. And 40 samples were used to test this algorithm of the model prospectively. The serum spectra were excited by laser of the wavelength 488.0 nm and 514.5 nm. The apparent differences of auto-fluorescence and Raman spectroscopy were observed for patients compared to the normal: the majority of the fluorescence spectra did not have violent alteration, but three Raman peaks had disappeared or very weak. For successful operation, the Raman spectrum of patient's serum is similar to the normal. Moreover, Delta lambda value(red shift of fluorescence peak) and alpha-value (rate of fluorescence intensity) also provide the reference for future research. And beta-value(rate of Raman peak intensity) will decrease with progression of the tumor. The result of spectrum analysis is accordance with the clinical diagnosis.