Spectral composition of irradiation regulates the cell growth and flavonoids biosynthesis in callus cultures of Saussurea medusa Maxim

Guo, Bin; Liu, Yonggang; Yan, Qiliang; Liu, Chun-Zhao

doi:10.1007/s10725-007-9192-0

Cited by 21 publications

(12 citation statements)

References 19 publications

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“…2). This is in agreement with previous reports, indicating the stimulatory effect of blue light on the accumulation of plant phenolics in in vitro cultures, with examples including flavonoids in Saussurea medusa calli (Guo et al 2007) and phenolic acids in shoots of Ruta graveolens (Szopa et al 2012). Unfortunately, in the present work the production of the most valuable metabolites (i.e.…”

supporting

confidence: 94%

Light and temperature conditions affect bioflavonoid accumulation in callus cultures of Cyclopia subternata Vogel (honeybush)

Kokotkiewicz

Buciński

Łuczkiewicz

2014

Plant Cell Tiss Organ Cult

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Callus cultures of the endemic South-African legume Cyclopia subternata were cultivated under varying light and temperature conditions to determine their influence on biomass growth and bioflavonoids accumulation. Experimental modifications of light included complete darkness, light of different spectral quality (white, red, blue and yellow) and ultraviolet C (UVC) irradiation. The calli were also subjected to elevated temperature or cold stress. Among the tested light regimes, cultivation under blue light resulted in the highest levels of hesperidin (H)-118.00 mg 100 g -1 dry weight (DW) on 28 days of experiment, as well as isoflavones: 7-O-b-glucosides of calycosin (CG), pseudobaptigenin (PG) and formononetin (FG)-28.74, 19.26 and 10.32 mg 100 g -1 DW, respectively, in 14-days old calli. UVC irradiation applied on 20 days stimulated the accumulation of H (204.14 mg 100 g -1 DW), CG (31.84 mg 100 g -1 DW) and PG (18.09 mg 100 g -1 DW) in 28 days culture by 140, 46 and 165 %, respectively, without negatively influencing callus growth. Low temperature (13°C) increased CG content by over 1,500 % (235.29 mg 100 g -1 DW) when applied during the whole 28-days growth cycle, at the same time causing 95 % decrease in culture growth in comparison to reference calli maintained at 24°C. On the contrary, elevated temperature (29°C) applied during the second half of the culture period resulted in over 300 and 500 % increase in CG and PG content (61.76 and 58.89 mg 100 g -1 , respectively) while maintaining relatively high biomass yield.

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confidence: 94%

Light and temperature conditions affect bioflavonoid accumulation in callus cultures of Cyclopia subternata Vogel (honeybush)

Kokotkiewicz

Buciński

Łuczkiewicz

2014

Plant Cell Tiss Organ Cult

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“…Although no previous information is available on the effects of light quality on these phenolic compounds, similar results have been reported for other phenolics under red light. Guo et al (2007) showed that flavonoid biosynthesis in Saussurea medusa callus cultures was inhibited by red light irradiation. Similarly, leaves of Ocimum basilicum (Shoji et al, 2011) irradiated by red light were found to contain lower concentrations of chicoric acid.…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, the phenolic content of buckwheat (Fagopyrum esculentum) leaves was significantly increased when the plants received a combination of red, green, and blue light (Hossen, 2007). In Saussurea medusa, a positive correlation was found between blue LEDs and the biosynthesis of flavonoids, whereas red LEDs inhibited flavonoid biosynthesis (Guo et al, 2007).…”

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confidence: 99%

Red Light-emitting Diode Light Irradiation Improves Root and Leaf Formation in Difficult-to-propagate Protea cynaroides L. Plantlets In Vitro

Wu¹,

Lin²

2012

horts

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The effects of light quality emitted by light-emitting diodes (LEDs) on the growth and morphogenesis, and concentrations of endogenous phenolic compounds of Protea cynaroides L. plantlets in vitro, were investigated. Plantlets were cultured under four light treatments: conventional fluorescent lamps (control), red LEDs (630 nm), blue LEDs (460 nm), and red + blue LEDs (1:1 photosynthetic photon flux). Four phenolic compounds extracted from the plantlets were analyzed: 3,4-dihydroxybenzoic acid, gallic acid, caffeic acid, and ferulic acid. The highest rooting percentage was observed in plantlets cultured under red LEDs (67%) compared with 7% under conventional white fluorescent light, 13% under blue LEDs, and 13% under red + blue LEDs. The highest number of roots per plantlet was also found under red LEDs, whereas a significantly lower number of roots per plantlet was obtained under the other light treatments. Furthermore, red light promoted the formation of new leaves in P. cynaroides plantlets. However, the highest leaf dry weight (53.8 mg per plantlet) was found in plantlets irradiated by the combination of red and blue LEDs. Phenolic analyses showed that the lowest concentrations of 3,4-dihydroxybenzoic acid (4.3 mg·g−1), gallic acid (7.0 mg·g−1), and ferulic acid (7.4 mg·g−1) were detected in plantlets exposed to red light, whereas those irradiated by white fluorescent light contained the highest concentration. A significant inverse correlation (r = –0.419) was established between 3,4-dihydroxybenzoic acid and rooting percentage. Strong inverse correlations were also established between 3,4-dihydroxybenzoic acid and number of roots per plantlet (r = –0.768) as well as between ferulic acid and number of roots per plantlet (r = –0.732). These results indicate that the stimulation of root formation in P. cynaroides plantlets under red LEDs is the result of the low endogenous concentrations of 3,4-dihydroxybenzoic acid and ferulic acid.

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“…Cultured cells could produce secondary metabolites, and the quantity of such a useful ingredient varies with the culture environment, including the kind and strength of light irradiating cultured cells (Guo et al, 2007;Ouyong et al, 2003). In this study, we expected to isolate the allelochemicals via abundant biosynthesis, so they could be released into the solvent by regulating the type and strength of light irradiating the calluses, because it is evident that asparagus calluses possess allelopathic activities.…”

Section: Resultsmentioning

confidence: 99%

“…It has been reported that artemisinin contents were increased under red light in the culture of hairy roots in Artemisia annua (Wang et al, 2001). In Cistanche deserticola and Saussurea medusa, phenylethanoid glycoside content (Ouyang et al, 2003) and the accumulation of flavonoid (Guo et al, 2007) could be promoted, respectively, in individual calluses by irradiation with blue light. These findings led us to anticipate that the cultures would actively produce allelochemicals by irradiation with a specific wavelength range of light.…”

Section: Introductionmentioning

confidence: 99%

Development of an in vitro System for the Evaluation of Allelopathic Activities of Asparagus Calluses

Watanabe

Iwai

Ono

et al. 2011

J. Japan. Soc. Hort. Sci.

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An aseptic bioassay system to estimate allelopathy in asparagus calluses was established. Allelopathic activities of calluses were measured according to the callus packed cell volume (PCV) by means of the sandwich method, and remarkable inhibition of acceptor plants was observed by the addition of over 0.1 mL PCV. When female and male calluses were separately used for the sandwich method, there was no sexual difference in allelopathic activity. To detect the allelopathic activities of the callus cells in detail, we improved the plant box method that substituted the calluses for the seedlings, and serious inhibition of acceptor plants was observed with the addition of over 0.35 mL PCV as donor material. Allelopathic activities were accelerated by irradiating the calluses with light-emitting diodes (LEDs) prior to performing the aseptic bioassay. It also seemed that the light quality would affect their activities. The allelopathic activity appeared intensely with irradiation of the calluses with a blue LED. The aseptic bioassay system that employed LED-irradiated calluses established here could accelerate effectively the isolation and identification of allelochemical(s).

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Spectral composition of irradiation regulates the cell growth and flavonoids biosynthesis in callus cultures of Saussurea medusa Maxim

Cited by 21 publications

References 19 publications

Light and temperature conditions affect bioflavonoid accumulation in callus cultures of Cyclopia subternata Vogel (honeybush)

Light and temperature conditions affect bioflavonoid accumulation in callus cultures of Cyclopia subternata Vogel (honeybush)

Red Light-emitting Diode Light Irradiation Improves Root and Leaf Formation in Difficult-to-propagate Protea cynaroides L. Plantlets In Vitro

Development of an in vitro System for the Evaluation of Allelopathic Activities of Asparagus Calluses

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