Spectral-Domain Optical Coherence Tomography as a Noninvasive Method to Assess Damaged and Regenerating Adult Zebrafish Retinas

Bailey, Travis J.; Davis, Darin H.; Vance, Joseph E.; Hyde, David R.

doi:10.1167/iovs.11-8895

Cited by 42 publications

(35 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1H) with exception to the distal aspect of the outer retina. As previously demonstrated by Bailey et al (Bailey et al, 2012). , additional bands (Figs.…”

Section: Resultssupporting

confidence: 76%

See 1 more Smart Citation

The adult zebrafish retina: In vivo optical sectioning with Confocal Scanning Laser Ophthalmoscopy and Spectral-Domain Optical Coherence Tomography

Bell

Yuan

DiCicco

et al. 2016

Experimental Eye Research

View full text Add to dashboard Cite

Non-invasive imaging is an invaluable diagnostic tool in ophthalmology. Two imaging devices, the scanning laser ophthalmoscope (SLO) and spectral domain optical coherence tomography (SDOCT), emerged from the clinical realm to provide research scientists with a real-time view of ocular morphology in living animals. We utilized these two independent imaging modalities in a complementary manner to perform in vivo optical sectioning of the adult zebrafish retina. Due to the very high optical power of the zebrafish lens, the confocal depth of field is narrow, allowing for detailed en face views of specific retinal layers, including the cone mosaic. Moreover, we demonstrate that both native reflectance, as well as fluorescent features observed by SLO, can be combined with axial in-depth information obtained by SDOCT. These imaging approaches can be used to screen for ocular phenotypes and monitor retinal pathology in a non-invasive manner.

show abstract

“…1H) with exception to the distal aspect of the outer retina. As previously demonstrated by Bailey et al (Bailey et al, 2012). , additional bands (Figs.…”

Section: Resultssupporting

confidence: 76%

“…In the LRP, two smaller but distinct peaks (arrow) can be seen in albinos but not in pigmented animals. These peaks are believed to represent rod outer segments (ROS) and the retinal pigment epithelium (RPE) (Bailey et al 2012). …”

Section: Resultsmentioning

confidence: 99%

The adult zebrafish retina: In vivo optical sectioning with Confocal Scanning Laser Ophthalmoscopy and Spectral-Domain Optical Coherence Tomography

Bell

Yuan

DiCicco

et al. 2016

Experimental Eye Research

View full text Add to dashboard Cite

show abstract

“…Importantly, OCT has also been employed to assay dynamic morphological changes during zebrafish retinal lamination and photoreceptor loss, and regeneration with light-induced or ouabaininduced damage in vivo. 98 It is possible to not only resolve individual retinal layers, from the retinal nerve fibre layer to the RPE, but also the photoreceptor mosaic, emphasising the high-resolution imaging capacity of OCT in small-animal imaging (Figure 4). This work potentiates the ability to study dynamic processes of zebrafish retinal development, damage, and regeneration whilst providing a means to characterise retinal degenerative disorders and assess the efficacy of potential treatments.…”

Section: Photoreceptor Regenerationmentioning

confidence: 99%

The zebrafish eye—a paradigm for investigating human ocular genetics

Richardson¹,

Tracey‐White²,

Webster

et al. 2016

Eye

153

162

View full text Add to dashboard Cite

Although human epidemiological and genetic studies are essential to elucidate the aetiology of normal and aberrant ocular development, animal models have provided us with an understanding of the pathogenesis of multiple developmental ocular malformations. Zebrafish eye development displays in depth molecular complexity and stringent spatiotemporal regulation that incorporates developmental contributions of the surface ectoderm, neuroectoderm and head mesenchyme, similar to that seen in humans. For this reason, and due to its genetic tractability, external fertilisation, and early optical clarity, the zebrafish has become an invaluable vertebrate system to investigate human ocular development and disease. Recently, zebrafish have been at the leading edge of preclinical therapy development, with their amenability to genetic manipulation facilitating the generation of robust ocular disease models required for large-scale genetic and drug screening programmes. This review presents an overview of human and zebrafish ocular development, genetic methodologies employed for zebrafish mutagenesis, relevant models of ocular disease, and finally therapeutic approaches, which may have translational leads in the future.

show abstract

“…[2][3][4] We chose a commercial hand-held portable platform approved for retinal imaging in pediatric human subjects 5,14,[20][21][22] and in basic animal research. [23][24][25] Furthermore, the largest ongoing randomized trial for age-related macular degeneration (AMD), the NEI-sponsored Age-Related Eye Disease Study 2, exclusively allows the Bioptigen SDOIS platform for its longitudinal, observational ancillary SDOCT study (AREDS2 Ancillary SDOCT Study). 6,26 The baseline dataset and measurements for both control and AMD eyes in this study has been made publicly available.…”

Section: -10mentioning

confidence: 99%

Lateral and axial measurement differences between spectral-domain optical coherence tomography systems

et al. 2014

View full text Add to dashboard Cite

Abstract. We assessed the reproducibility of lateral and axial measurements performed with spectral-domain optical coherence tomography (SDOCT) instruments from a single manufacturer and across several manufacturers. One human retina phantom was imaged on two instruments each from four SDOCT platforms: Zeiss Cirrus, Heidelberg Spectralis, Bioptigen SDOIS, and hand-held Bioptigen Envisu. Built-in software calipers were used to perform manual measurements of a fixed lateral width (LW), central foveal thickness (CFT), and parafoveal thickness (PFT) 1 mm from foveal center. Inter-and intraplatform reproducibilities were assessed with analysis of variance and Tukey-Kramer tests. The range of measurements between platforms was 5171 to 5290 μm for mean LW (p < 0.001), 162 to 196 μm for mean CFT (p < 0.001), and 267 to 316 μm for mean PFT (p < 0.001). All SDOCT platforms had significant differences between each other for all measurements, except LW between Bioptigen SDOIS and Envisu (p ¼ 0.27). Intraplatform differences were significantly smaller than interplatform differences for LW (p ¼ 0.020), CFT (p ¼ 0.045), and PFT (p ¼ 0.004). Conversion factors were generated for lateral and axial scaling between SDOCT platforms. Lateral and axial manual measurements have greater variance across different SDOCT platforms than between instruments from the same platform. Conversion factors for measurements from different platforms can produce normalized values for patient care and clinical studies.

show abstract

Spectral-Domain Optical Coherence Tomography as a Noninvasive Method to Assess Damaged and Regenerating Adult Zebrafish Retinas

Cited by 42 publications

References 36 publications

The adult zebrafish retina: In vivo optical sectioning with Confocal Scanning Laser Ophthalmoscopy and Spectral-Domain Optical Coherence Tomography

The adult zebrafish retina: In vivo optical sectioning with Confocal Scanning Laser Ophthalmoscopy and Spectral-Domain Optical Coherence Tomography

The zebrafish eye—a paradigm for investigating human ocular genetics

Lateral and axial measurement differences between spectral-domain optical coherence tomography systems

Contact Info

Product

Resources

About