Spectral fingerprinting of cellular lipid droplets using stimulated Raman scattering microscopy and chemometric analysis

Abstract

Hyperspectral stimulated Raman scattering (SRS) microscopy coupled to spectral phasor analysis is a powerful method for the detection of fatty acids in solution and in cellular lipid droplets.

Label-Free Screening of Drug-Induced Liver Injury Using Stimulated Raman Scattering Microscopy and Spectral Phasor Analysis

“… 18 The spectral phasor therefore represents a density plot of the ensemble pixels which are clustered based on spectral similarity, thereby simplifying the interpretation and segmentation of hyperspectral imaging data sets. Previous studies have highlighted the application of spectral phasor analysis for visualizing the phenotypic outcomes of drug treatment, 21 , 26 , 28 imaging ratiometric alkyne sensors, 22 and for investigating cellular mitosis. 28 In each of these studies, the biomolecular content of each cluster in the spectral phasor plot was projected back onto the original data set.…”

“…Hyperspectral SRS imaging has become a high-content cellular imaging platform and the application of chemometric analysis techniques can extricate the underlying spectral changes associated with drug treatment or disease progression. In particular, multivariate curve resolution, k -means cluster analysis , and spectral phasor analysis − have recently been applied to hyperspectral SRS imaging data for cellular and tissue studies.…”

Raman Imaging—A Valuable Tool for Tracking Fatty Acid Metabolism—Normal and Cancer Human Colon Single-Cell Study

“…Research into the detection of fatty acids, their storage sites or the tracking of fat metabolism products has been successfully carried out by various research groups from around the world. Lipid metabolism and its mechanism have been the subject of research by scientists from all over the world; however, Raman spectroscopy is a unique method in this aspect as it gives very precise, unambiguous results, allowing the registration of even the smallest changes occurring in the cell as a result of fatty acid supplementation [45][46][47][48][49][50][51][52][53].…”