Spectral fingerprinting of polycyclic aromatic hydrocarbons in high-volume ambient air samples by constant energy synchronous luminescence spectroscopy

Kerkhoff, M. Jonell; Lee, T.M.; Allen, Eric R.; Lundgren, Dale A.; Winefordner, J. D.

doi:10.1021/es00138a007

Cited by 24 publications

(2 citation statements)

References 26 publications

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“…Samples of these compounds are very often complex and usually require extensive identification and quantitation procedures. Many methods have utilized a combination of a glass or quartz fiber filter with a polyurethane foam plug to collect PAH (151)(152)(153).…”

Section: Polynuclear and Polycyclic Aromatic Hydrocarbonsmentioning

confidence: 99%

Industrial hygiene

Langhorst¹,

Coyne²

1987

Anal. Chem.

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Section: Polynuclear and Polycyclic Aromatic Hydrocarbonsmentioning

confidence: 99%

Industrial hygiene

Langhorst¹,

Coyne²

1987

Anal. Chem.

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“…This approach has been successfully applied in PAH analysis. [9][10][11][12][13][14][15][16][17][18] In CESLS, the synchronous scanning can be optimized for a specific absorption-fluorescence transition by setting a ∆ -υ value equal to the vibrational energy difference. CESLS is more effective for narrowing the spectral band and reducing Raman scattering interference than general SFS.…”

mentioning

confidence: 99%

Micelle-Sensitized Constant-Energy Synchronous Fluorescence Spectrometry for the Simultaneous Determination of Pyrene, Benzo[a]pyrene and Perylene

Lin

2005

ANAL. SCI.

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The identification and quantification of polycyclic aromatic hydrocarbons (PAHs) in the environment are of great interest due to their carcinogenic and mutagenic characteristics. 1 Chromatographic methods, like GC-MS, HPLC with UV-visible or fluorescence detector etc., are time-consuming, expensive and require a separation procedure before analysis. Because of the strong intrinsic fluorescence emission of PAHs, fluorescence spectroscopy could provide a highly sensitive method for the analysis of PAHs.Synchronous fluorescence spectroscopy (SFS) has been known to be a simple and selective method for the simultaneous determination of multicomponent samples, 2-6 while maintainning the fluorometric sensitivity.Inman and Winefordner 7,8 described a kind of synchronous scanning mode, namely constant-energy synchronous luminescence spectrometry (CESLS), in which a constant energy difference (∆ -υ) between excitation and emission monochromators is maintained as each is scanned through the spectral region of interest. This approach has been successfully applied in PAH analysis. [9][10][11][12][13][14][15][16][17][18] In CESLS, the synchronous scanning can be optimized for a specific absorption-fluorescence transition by setting a ∆ -υ value equal to the vibrational energy difference. CESLS is more effective for narrowing the spectral band and reducing Raman scattering interference than general SFS. The fluorescence excitation and emission spectra of PAHs are generally characterized with similar spaced vibrational features. CESLS can attain the same spectral features with a single scan as conventional constant-wavelength synchronous fluorescence spectroscopy in PAH multicomponent analysis.However, resonance electronic energy transfer in mixture of PAHs has limited the application of SFS for PAH analysis. 19 For example, there is appreciable energy transfer from pyrene to perylene in a solution containing both. 20,21 The micellization of a PAHs mixture solution can help resolve this problem by isolating fluorophores in different micelles. 22,23 Micelles can also easily solubilize PAHs and increase the fluorescence intensities of fluorophores, thereby increasing the sensitivity. The analysis of certain PAHs mixtures in micellar media has been made using conventional fluorometric techniques, 22,24,25 as well as constant-wavelength and variable-angle synchronous fluorometry. 23,26,27 It can be expected that CESLS would encounter the same problem of energy transfer, and micelles would be a good solution, as in other fluorometric methods.In this paper, combined use of micellar media and constantenergy synchronous fluorescence spectroscopy for the simultaneous determination of pyrene, perylene and benzo [a]pyrene in water samples is described.To our knowledge, this is the first report on the application of micellar media in CESLS. The method exempted the resonance electronic energy transfer among the different PAHs, and thus allowed their simultaneous identification and quantitative determination via a single spectrum. Because th...

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Highly sensitive and selective spectrofluorimetric approach for the rapid determination of trace benzo[α]pyrene in drinking water and in solutions leached from disposable paper cups

Zhou

Luo²,

et al. 2011

Luminescence

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Based on the excellent band narrowing and background suppressing features of second-derivative constant-energy synchronous spectrofluorimetry with a Δ⁻υ value of 1400 cm(-1) , the strong fluorescence intensity for benzo[α]pyrene (BaP) obtained in dichloromethane and the use of standard addition method, a highly sensitive and selective approach for the quantitative determination of trace amount of BaP in drinking water has been established in this study. The detection and quantification limits were 0.11 and 0.37 ng L(-1) , respectively, and the recoveries obtained from spiked Milli-Q water, bottled natural spring water, tank-purified water and tap water at different concentrations, ranged from 86.0 to 104.0%. This method has been applied for the determination of trace BaP in solution leached from disposable paper cups. The experimental results indicated that BaP was leached from paper cups when filled with hot water, but it was not detected when cool (unheated) water was used.

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Spectral fingerprinting of polycyclic aromatic hydrocarbons in high-volume ambient air samples by constant energy synchronous luminescence spectroscopy

Cited by 24 publications

References 26 publications

Industrial hygiene

Industrial hygiene

Micelle-Sensitized Constant-Energy Synchronous Fluorescence Spectrometry for the Simultaneous Determination of Pyrene, Benzo[a]pyrene and Perylene

Highly sensitive and selective spectrofluorimetric approach for the rapid determination of trace benzo[α]pyrene in drinking water and in solutions leached from disposable paper cups

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