Spectral imaging of single molecules by transmission grating-based epi-fluorescence microscopy

Han, Rui; Zhang, Ye-Wang; Dong, Xin; Gai, Hongluei; Yeung, Edward S.

doi:10.1016/j.aca.2008.05.017

Cited by 21 publications

(34 citation statements)

References 34 publications

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“…Single QDs spectral imaging was described by our previous work (Han et al 2008;Chen et al 2009;Shi et al 2010). A transmission grating with 70 lines/mm placed in front of the EMCCD camera can separate the fluorescence imaging into zeroth-order spots and firstorder spectral streak.…”

Section: Resultsmentioning

confidence: 99%

Excitation wavelength and intensity dependence of photo-spectral blue shift in single CdSe/ZnS quantum dots

et al. 2014

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The influence of excitation wavelength and intensity on core/shell CdSe/ZnS quantum dots (QDs) photo-spectral blue shift was investigated by spectral imaging. Analysis of the evolution of the distance between the zeroth-order spot and the firstorder spectral streak, we found that the extent of blue shift strongly depends on the excitation wavelength and QDs sizes, but not on the excitation intensity. Converted the extent of blue shift into the decreased QDs volume at a series of time, the core oxidation kinetics of CdSe/ZnS QDs was uncovered that provided a quantitative comparison method for study the excitation wavelength and intensity dependence of single QDs blue shift. The core oxidation rate is almost proportional to the excitation intensity. These results are explained by a fact that higher energy excitation wavelength can accelerate individual exciton formation and higher excitation intensity can induce more amount of exciton formation per a unit time.

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Section: Resultsmentioning

confidence: 99%

Excitation wavelength and intensity dependence of photo-spectral blue shift in single CdSe/ZnS quantum dots

et al. 2014

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“…The photo-oxidation of each individual QD starts at different times and processes differently because of the heterogeneity in synthesis and in orientation of the dipole moments of QDs, which causes asynchrony of the blue shift. The blue shifts of QDs are easily observed and monitored with the help of a transmission grating inserted between the sample and EMCCD, as reported in our previous work [15][16][17]. The schematic is shown in Fig.…”

Section: Resultsmentioning

confidence: 82%

“…In our previous work, we have demonstrated that spectral imaging microscopy can be used to monitor the spectra and the spectral shift of a single QD [15][16][17]. We have also obtained superlocalization imaging of a multicolor QD complex by random blinking and different emission wavelengths of QDs using the same technique [18].…”

Section: Introductionmentioning

confidence: 99%

Spectral imaging superlocalization microscopy for quantum dots

Shi

Zhao

et al. 2015

Sensors and Actuators B: Chemical

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“…The spectral imaging methodology used is similar to the one used previously for fluorescence imaging of single molecules. 14 Briefly, the only modification to a conventional upright light microscope was incorporation of a diffraction grating in the light path. Initially, a transmission grating was placed between the CCD camera and microscope objective on the glass window, which was above the tube lens, at a distance of ∼ 10.5 cm from the camera chip.…”

Section: Methodsmentioning

confidence: 99%

“…A high-throughput spectral imaging method based on a transmission diffraction grating was used to measure fluorescence spectra of single molecules and fluorescently labeled cells that flow through a capillary. 9,13,14 Another setup based on a reflective diffraction grating was developed recently to record absorption spectra of dyes and was featured as a simple and inexpensive alternative to commercially available spectral microscopes. 18 The presented work outlines a spectral imaging setup based on a transmission diffraction grating that can be used for fluorescence as well as absorption (colorimetric) and scattering (darkfield) spectral imaging of cells.…”

Section: Introductionmentioning

confidence: 99%

Multimodal Spectral Imaging of Cells Using a Transmission Diffraction Grating on a Light Microscope

et al. 2011

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A multimodal methodology for spectral imaging of cells is presented. The spectral imaging setup uses a transmission diffraction grating on a light microscope to concurrently record spectral images of cells and cellular organelles by fluorescence, darkfield, brightfield, and differential interference contrast (DIC) spectral microscopy. Initially, the setup was applied for fluorescence spectral imaging of yeast and mammalian cells labeled with multiple fluorophores. Fluorescence signals originating from fluorescently labeled biomolecules in cells were collected through triple or single filter cubes, separated by the grating, and imaged using a charge-coupled device (CCD) camera. Cellular components such as nuclei, cytoskeleton, and mitochondria were spatially separated by the fluorescence spectra of the fluorophores present in them, providing detailed multi-colored spectral images of cells. Additionally, the grating-based spectral microscope enabled measurement of scattering and absorption spectra of unlabeled cells and stained tissue sections using darkfield and brightfield or DIC spectral microscopy, respectively. The presented spectral imaging methodology provides a readily affordable approach for multimodal spectral characterization of biological cells and other specimens.

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Spectral imaging of single molecules by transmission grating-based epi-fluorescence microscopy

Cited by 21 publications

References 34 publications

Excitation wavelength and intensity dependence of photo-spectral blue shift in single CdSe/ZnS quantum dots

Excitation wavelength and intensity dependence of photo-spectral blue shift in single CdSe/ZnS quantum dots

Spectral imaging superlocalization microscopy for quantum dots

Multimodal Spectral Imaging of Cells Using a Transmission Diffraction Grating on a Light Microscope

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