Spectral imaging technology of epithelial tissue based on two-photon excited fluorescence and second-harmonic generation

Luo, Tian; Chen, Jianxin; Zhuo, Shuangmu; Jiang, Xingshan; Chen, Huanglin; Luo, Teng; Zou, Qingze

doi:10.1007/s12200-008-0031-3

Cited by 3 publications

(5 citation statements)

References 17 publications

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“…Recently, multiphoton microscopy (MPM) has been applied to a variety of imaging field based on intrinsic two‐photon excited fluorescence (TPEF) and second harmonic generation (SHG; Zipfel et al ., ; Luo et al ., ; Zhuo et al., ; Cicchi et al., ; Zhu et al ., ; Yan et al ., ). It has several advantages including deeper penetration depth, higher resolution, inherent optical sectioning and minimal phototoxicity compared with traditional optical imaging.…”

Section: Introductionmentioning

confidence: 99%

Differentiating the two main histologic categories of fibroadenoma tissue from normal breast tissue by using multiphoton microscopy

Nie

et al. 2015

Journal of Microscopy

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Multiphoton microscopy has become a novel biological imaging technique that allows cellular and subcellular microstructure imaging based on two-photon excited fluorescence and second harmonic generation. In this work, we used multiphoton microscopy to obtain the high-contrast images of human normal breast tissue and two main histologic types of fibroadenoma (intracanalicular, pericanalicular). Moreover, quantitative image analysis was performed to characterize the changes of collagen morphology (collagen content, collagen orientation). The results show that multiphoton microscopy combined with quantitative method has the ability to identify the characteristics of fibroadenoma including changes of the duct architecture and collagen morphology in stroma. With the advancement of multiphoton microscopy, we believe that the technique has great potential to be a real-time histopathological diagnostic tool for intraoperative detection of fibroadenoma in the future.

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Section: Introductionmentioning

confidence: 99%

Differentiating the two main histologic categories of fibroadenoma tissue from normal breast tissue by using multiphoton microscopy

Nie

et al. 2015

Journal of Microscopy

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“…It is well known that the autofluorescence in the visible range using two-photon excitation primarily originates from NADH, FAD, and keratin. [22][23][24][25][26] In this specific study, the visualization of Ca 2þ -induced morphological changes was of primary interest. It should be noted that similar morphological features could potentially be recognized with other optical modalities, e.g., OCT and RCM.…”

Section: Discussionmentioning

confidence: 99%

“…21 When applied to noninvasive and label-free investigations of the skin, MPM is primarily based on two-photon excitation of intrinsic cellular fluorophores, such as nicotinamide adenine dinucleotide (NADH), flavin adenine dinucleotide (FAD), and keratin. [22][23][24][25][26] Additional MPM modalities, such as fluorescence lifetime imaging (FLIM) or spectral detection, [27][28][29] would enable further fluorophore separation. This can provide information regarding cellular metabolism 30 to complement morphological data, making MPM a suitable noninvasive imaging technology to use in combination with in vitro tissue culturing.…”

Section: Introductionmentioning

confidence: 99%

Monitoring calcium-induced epidermal differentiation in vitro using multiphoton microscopy

2020

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Significance: Research in tissue engineering and in vitro organ formation has recently intensified. To assess tissue morphology, the method of choice today is restricted primarily to histology. Thus novel tools are required to enable noninvasive, and preferably label-free, three-dimensional imaging that is more compatible with futuristic organ-on-a-chip models. Aim: We investigate the potential for using multiphoton microscopy (MPM) as a label-free in vitro approach to monitor calcium-induced epidermal differentiation. Approach: In vitro epidermis was cultured at the air-liquid interface in varying calcium concentrations. Morphology and tissue architecture were investigated using MPM based on visualizing cellular autofluorescence. Results: Distinct morphologies corresponding to epidermal differentiation were observed. In addition, Ca 2þ-induced effects could be distinguished based on the architectural differences in stratification in the tissue cultures. Conclusions: Our study shows that MPM based on cellular autofluorescence enables visualization of Ca 2þ-induced differentiation in epidermal skin models in vitro. The technique has potential to be further adapted as a noninvasive, label-free, and real-time tool to monitor tissue regeneration and organ formation in vitro.

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“…The relative standard deviation was less than 4.6% to five parallel measurements of same sample, and the recovery was in the range of 98 -106%. As compared with the method of HPLC (the linear range, within 0.50 -5.0 μg/L; the recovery and RSD, 99.7 -108% (n = 6) and 1.0%; retention time, 12 min), 9 the proposed method is clearly applicable for determination of DPH in pharmaceutical samples mixed with ephedrine at thresholds above 1.20 μg/L and has a wider detecting range. Though it comes short of a simultaneously detecting ability for DPH and EPH, the method has advantages over HPLC method in terms of speed and convenience, economics, safe procedure and good sensitivity, and could be an alternative for places where HPLC equipment is not available.…”

Section: +mentioning

confidence: 93%

“…The comparison HPLC analysis was performed with a P-3000 liquid chromatograph (Chuangxin Tongheng Science & Technology Co. Ltd., Beijing, China) in accordance with the literature. 9 …”

Section: Methodsmentioning

confidence: 99%

Selective Determination of Diphenhydramine in Compound Pharmaceutical Containing Ephedrine by Flow-Injection Electrochemiluminescence

et al. 2008

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The development of compounding drugs by mixed Chinese traditional and western medicine for treating disease has became important in China. Chinese tradition medicine was crude drugs with low side-effect and long-effect, but western medicine as chemical medicine usually effect in a short time and the aftereffect can be reversed of using Chinese traditional medicine. Many clinical and pharmacological experiments have proved that compounding drugs has the advantages of improving curative effects, reducing deleterious side-effects and shortening the period of treatment in relation to using Chinese traditional or western medicine separately.Diphenhydramine, 2-(diphenylmethoxy)-N,N-dimethylethanamine hydrochloride (DPH), a histamine H 1 -receptor antagonist is widely used as antiallergic, antiemetic and antitussive drug in many pharmaceutical preparations. 1 DPH is usually given in a preparation of tablet or drops or as an injection, either alone or in combination with other drugs (i.e. ephedrine, EPH). EPH is generally prepared from Ma huang, which is a traditional Chinese medicine derived from Ephedrae plant. It is used as a pharmaceutical for exciting the central nervous system, the systole of blood vessels, and the lysis of spasm of bronchial smooth muscles. Excessive DPH use could bring about symptoms of phrenitis, tic, tremor, low blood pressure and decompensation. As for babies and young children, it also could result in exciting them easily and causing hallucinations. Mixing DPH with EPH to create a compound drug can exert the good qualities of two drugs and reduce the side-effects of using DPH alone. In the pharmaceutical compound of DPH and EPH, the amount of DPH must be accurately monitored to avoid possible damage to human health due to an unreasonable mixture. Many analytical methods have been proposed for determining DPH in pharmaceutical samples including titrimetry, 2 fluorophotometry, 3 electrochemical analysis 4 and spectrophotometry, 5,6 gas chromatography, 7 HPLC 8,9 and capillary electrophoresis. 10,11 Indeed, the spectrophotometry and HPLC are used as a pharmacopoeial method. 8,12 Nevertheless, the spectrophotometric methods are time consuming, especially for compounding a sample without prior separation; and those instruments are relatively high in cost. Additionally, these methods usually require extraction and complicated pretreatment procedures prior to analysis, resulting in use of some toxic reagents and excessive operation. Accordingly, to develop a rapidly and conveniently quantificational method of DPH in a pharmaceutical compound containing EPH has become a significant work.Flow injection analysis (FIA) is well established as an excellent technique for rapid automated quantitative analysis that combines on-line chemical and physical sample treatments with a range of flow-through detection systems in an enclosed, continuous flow environment.13 FIA does not require high cost equipment to overcome shortages of instruments in places where HPLC instruments are not available. On the other han...

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Spectral imaging technology of epithelial tissue based on two-photon excited fluorescence and second-harmonic generation

Cited by 3 publications

References 17 publications

Differentiating the two main histologic categories of fibroadenoma tissue from normal breast tissue by using multiphoton microscopy

Differentiating the two main histologic categories of fibroadenoma tissue from normal breast tissue by using multiphoton microscopy

Monitoring calcium-induced epidermal differentiation in vitro using multiphoton microscopy

Selective Determination of Diphenhydramine in Compound Pharmaceutical Containing Ephedrine by Flow-Injection Electrochemiluminescence

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