Spectral karyotyping of the human colon cancer cell lines SW480 and SW620

Melcher, Ralph; Luehrs, Hardi; Steinlein, Claus; Feichtinger, W.; Mueller, C.W.; Schmid, Michael; Scheppach, Wolfgang; Menzel, Thomas P.

doi:10.1016/s0016-5085(00)82310-0

Cited by 3 publications

(12 citation statements)

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“…47 This anomaly could not have been identified only by the SKY technique. 48 Also, the t(10;12)(p13;q12) found by Gagos et al 47 and the t(10;3)(p13;q21) described by Melcher et al 48 are now known to be a t(3;10;12)(3qter/3q21T12?T10p15/10q26T10q24/ 10qter). Finally, an alteration on chromosome 19 45 Tomita et al 46 and Gagos et al 47 but they could not identify the origin of the translocated material.…”

Section: Cytogenetic Characterization Of the Sw480 Cell Linementioning

confidence: 91%

“…Some rearrangements remained unclear, and other anomalies were not detected by previous studies. [45][46][47][48] We believe that this might be due to the complexity of the rearrangements and to the fact that these authors used only one technique to solve the SW480 karyotype, i.e. spectral karyotyping (SKY) 48 or GTG banding.…”

Section: Cytogenetic Characterization Of the Sw480 Cell Linementioning

confidence: 99%

“…[45][46][47][48] We believe that this might be due to the complexity of the rearrangements and to the fact that these authors used only one technique to solve the SW480 karyotype, i.e. spectral karyotyping (SKY) 48 or GTG banding. [45][46][47] We used the combination of m-FISH and GTG banding on chromosomes, leading to a complete identification of all aberrations (Figure 1(a) and (b)).…”

Section: Cytogenetic Characterization Of the Sw480 Cell Linementioning

confidence: 99%

“…Finally, an alteration on chromosome 19 45 Tomita et al 46 and Gagos et al 47 but they could not identify the origin of the translocated material. Melcher et al 48 first identified the rearrangement as a complex four ways translocation (t (19;8;19;5)). After FISH using specific chromosome painting (data not shown), m-FISH and G-banding, we are finally able to describe the derivative as a t(5;8;19)(19pter/ 19q13.1T8?T 19q13.2/19q13.3T5q34/5qter).…”

Section: Cytogenetic Characterization Of the Sw480 Cell Linementioning

confidence: 99%

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SW480, a p53 Double-mutant Cell Line Retains Proficiency for Some p53 Functions

Rochette

Bastien

Lavoie

et al. 2005

Journal of Molecular Biology

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Section: Cytogenetic Characterization Of the Sw480 Cell Linementioning

confidence: 91%

Section: Cytogenetic Characterization Of the Sw480 Cell Linementioning

confidence: 99%

Section: Cytogenetic Characterization Of the Sw480 Cell Linementioning

confidence: 99%

Section: Cytogenetic Characterization Of the Sw480 Cell Linementioning

confidence: 99%

See 2 more Smart Citations

SW480, a p53 Double-mutant Cell Line Retains Proficiency for Some p53 Functions

Rochette

Bastien

Lavoie

et al. 2005

Journal of Molecular Biology

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“…Sequencing in coding regions of tumor DNA from 470 genes shows that sporadic colorectal cancers do not display a mutator phenotype at the nucleotide level (Wang et al 2002). Colorectal carcinomas mostly show highly complex karyotypes and rearrangements (Melcher et al 2000;Bomme et al 2001), aneuploidy and gene amplification (Fearon et al 1987;Jen et al 1994). Balanced structural rearrangements are rarely seen and the more complex karyotypes are dominated by various chromosomal imbalances, frequently including loss of chromosomal arms 8p, 17p 18p, 8q and gains of 6q, 7p, 7q, 8q, 11p, 13q, 20 p and 20q (Aust et al 2000;Nakao et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Topography of genetic loci in the nuclei of cells of colorectal carcinoma and adjacent tissue of colonic epithelium

Lukášová¹,

Kozubek²,

Falk³

et al. 2004

Chromosoma

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To determine the influence of increased gene expression and amplification in colorectal carcinoma on chromatin structure, the nuclear distances between pairs of bacterial artificial chromosome (BAC) clones with genomic separation from 800 to 29,000 kb were measured and compared between the tumor and parallel epithelial cells of six patients. The nuclear distances were measured between the loci in chromosomal bands 7p22.3-7p21.3; 7q35-7q36.3; 11p15.5-11p15.4; 20p13; 20p12.2; 20q11.21 and 20q12 where increased expression had been found in all types of colorectal carcinoma. The loci were visualized by three-dimensional fluorescence in situ hybridization using 22 BAC clones. Our results show that for short genomic separations, mean nuclear distance increases linearly with increased genomic separation. The results for some pairs of loci fell outside this linear slope, indicating the existence of different levels of chromatin folding. For the same genomic separations the nuclear distances were frequently shorter for tumor as compared with epithelial cells. Above the initial growing phase of the nuclear distances, a plateau phase was observed in both cell types where the increase in genomic separation was not accompanied by an increase in nuclear distance. The ratio of the mean nuclear distances between the corresponding loci in tumor and epithelium cells decreases with increasing amplification of loci. Our results further show that the large-scale chromatin folding might differ for specific regions of chromosomes and that it is basically preserved in tumor cells in spite of the amplification of many loci.

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Definitive molecular cytogenetic characterization of 15 colorectal cancer cell lines

Knutsen

Padilla‐Nash

Wangsa

et al. 2009

Genes Chromosomes & Cancer

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In defining the genetic profiles in cancer, cytogenetically aberrant cell lines derived from primary tumors are important tools for the study of carcinogenesis. We here present the results of a comprehensive investigation of 15 established colorectal cancer cell lines utilizing spectral karyotyping (SKY), fluorescence in situ hybridization, and comparative genomic hybridization (CGH). Detailed karyotypic analysis by SKY on five of the lines (P53HCT116, T84, NCI-H508, NCI-H716, and SK-CO-1) are described here for the first time. The five lines with karyotypes in the diploid range and that are characterized by defects in DNA mismatch repair had a mean of 4.8 chromosomal abnormalities per line, whereas the 10 aneuploid lines exhibited complex karyotypes and a mean of 30 chromosomal abnormalities. Of the 150 clonal translocations, only eight were balanced and none were recurrent among the lines. We also reviewed the karyotypes of 345 cases of adenocarcinoma of the large intestine listed in the Mitelman Database of Chromosome Aberrations in Cancer. The types of abnormalities observed in the cell lines reflected those seen in primary tumors: there were no recurrent translocations in either tumors or cell lines, isochromosomes were the most common recurrent abnormalities, and breakpoints occurred most frequently at the centromeric/pericentromeric and telomere regions. Of the genomic imbalances detected by array CGH, 87% correlated with chromosome aberrations observed in the SKY studies. The fact that chromosome abnormalities result predominantly in copy number changes rather than specific chromosome or gene fusions, suggests this may be the major mechanism leading to carcinogenesis in colorectal cancer.

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Spectral karyotyping of the human colon cancer cell lines SW480 and SW620

Cited by 3 publications

References 0 publications

SW480, a p53 Double-mutant Cell Line Retains Proficiency for Some p53 Functions

SW480, a p53 Double-mutant Cell Line Retains Proficiency for Some p53 Functions

Topography of genetic loci in the nuclei of cells of colorectal carcinoma and adjacent tissue of colonic epithelium

Definitive molecular cytogenetic characterization of 15 colorectal cancer cell lines

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