Spectral polarization imaging of human rectum-membrane-prostate tissues

Wang, W.B.; Ali, J. H.; Alfano, R. R.; Vitenson, Jack H.; Lombardo, Joseph M.

doi:10.1109/jstqe.2003.813301

Cited by 16 publications

(4 citation statements)

References 8 publications

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“…Nowadays, this technique is included in the most expensive microscopes and is used to explore the spatial orientation patterns of individual macromolecules, to determine localization and directional orientation of collagen fibres, for topographic analysis and for imaging through turbid media among others (Speer & Dahners, 1979; Gleyzes et al ., 1997; Kocsis et al ., 1998; Forkey et al ., 2005; Yaroslavsky et al ., 2005). It has been reported that polarizing microscopes improve the detection of tiny structures in different tissues (Wolman, 1975; Sun et al ., 2001; Oron et al ., 2003; Wang et al ., 2003; Yaroslavsky et al ., 2005). In multi‐photon microscopy, polarization also helps to enhance the visibility of features and sub‐surface structures (Higgins et al ., 1996; Sun et al ., 2005).…”

Section: Discussionmentioning

confidence: 99%

Enhancement of confocal microscopy images using Mueller‐matrix polarimetry

Bueno

Cookson

Kisilak

et al. 2009

Journal of Microscopy

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Summary A simplified procedure based on Mueller‐matrix polarimetry has recently been reported as a method of retinal image improvement in a confocal ophthalmoscope [J. M. Bueno et al., J. Opt. Soc. Am. A 24, 1337 (2007)]. Here, we have applied the technique to imaging static samples providing well‐defined reflection properties. The method uses a generator of polarization states in the illumination pathway of a confocal scanning laser system. From the calculated four elements of the Mueller matrix of any sample and instrument combination, the best images defined by different metrics were constructed. For samples with specular, diffuse and mixed reflections, the best‐constructed images showed an enhancement in both objective and subjective image quality compared to the original images and those obtained from frame averaging. This technique could improve microscopic imaging in many diverse fields, particularly in biomedical imaging.

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Section: Discussionmentioning

confidence: 99%

Enhancement of confocal microscopy images using Mueller‐matrix polarimetry

Bueno

Cookson

Kisilak

et al. 2009

Journal of Microscopy

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“…In fact, light attributes, such as polarization, can also provide important information for target detection. Polarization information shows the surface features, shape, and so on of the water targets [9]. Thus, we will utilize polarization imaging for water surface, which is beneficial to water surface target information acquisition.…”

Section: Overall Design Of the Presented Frameworkmentioning

confidence: 99%

Polarization Imaging Target Detection Method by Imitating Dragonfly Compound Eye LF-SF Mechanism

Wang

Yan

et al. 2013

AMM

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Recently, water surface target detection and tracking for sea, lake, or river are challenging research topics. This paper presents a framework of target detection and tracing based on three-channel synchronization polarization imaging and imitation dragonfly compound eye LF-SF (large field-small field) mechanism. This framework can make full use of the advantages of polarization sensitivity of the compound eyes of a dragonfly, and be useful for effective water surface target detection and motion vector estimation.

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“…Our methods are concentrated on layers of cells near the surface, so that a time of ∼10 min for soaking samples in Cybesin (Cytate) solution was used [25]. Because of the mean mucosa thickness of 830 AE 60 μm and the mean rectal wall thickness of 2:57 AE 0:15 mm [26,27], the NIR pumping light of 750 to 800 nm can penetrate the rectal wall to reach the prostate for optical imaging if irradiating from the rectum [10,27]. Previous spectral polarized imaging experiments performed at the Institute for Ultrafast Spectroscopy and Lasers show that an ICG-stained small object hidden inside the host prostate tissues in the rectum membrane/prostate structures at depths of 7:5 mm can be imaged and identified using fluorescence imaging methods [27].…”

Section: Fluorescence Polarization Imagingmentioning

confidence: 99%

Study of rotational dynamics of receptor-targeted contrast agents in cancerous and normal prostate tissues using time-resolved picosecond emission spectroscopy

Wang

Achilefu

et al. 2011

Appl. Opt.

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We studied the time-resolved polarization-dependent fluorescence spectroscopy of receptor-targeted contrast agents (Cybesin and Cytate) bound with prostate cancer cells in prostate tissue. An analytical model dealing with highly viscous tissue media was developed and used to investigate the rotation times and fluorescence anisotropies of the receptor-targeted contrast agents in prostate tissue. The differences of rotation times and fluorescence anisotropies were observed for Cybesin (Cytate) in cancerous and normal prostate tissues, which reflect changes of the microstructures of cancerous and normal tissues and their different bound affinity with contrast agents. The preferential uptake of Cytate (Cybesin) in cancerous tissue was used to image and distinguish cancerous tissue areas from normal tissue areas. The fluorescence polarization difference imaging technique was used to enhance the image contrast between the cancerous and normal tissue areas. This research may help to introduce a new optical approach and criteria for prostate cancer detection.

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Spectral polarization imaging of human rectum-membrane-prostate tissues

Cited by 16 publications

References 8 publications

Enhancement of confocal microscopy images using Mueller‐matrix polarimetry

Enhancement of confocal microscopy images using Mueller‐matrix polarimetry

Polarization Imaging Target Detection Method by Imitating Dragonfly Compound Eye LF-SF Mechanism

Study of rotational dynamics of receptor-targeted contrast agents in cancerous and normal prostate tissues using time-resolved picosecond emission spectroscopy

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