Spectral sensitivity of guppy visual pigments reconstituted in vitro to resolve association of opsins with cone cell types

Kawamura, Shoji; Kasagi, Satoshi; Kasai, Daisuke; Tezuka, Ayumi; Shoji, Ayako; Takahashi, Akiyoshi; Imai, Hiroo; Kawata, Masakado

doi:10.1016/j.visres.2016.06.013

Cited by 35 publications

(50 citation statements)

References 39 publications

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“…In bony fishes, the opsin repertoire differs among species (Gojobori & Innan, 2009; Yokoyama, 2000). Generally, the surface‐dwelling species, which habit the luminous superficial area of water, have a large cone opsin repertoire, as seen in zebrafish ( Danio rerio ) (Chinen, Hamaoka, Yamada, & Kawamura, 2003), medaka ( Oryzias latipes ) (Matsumoto, Fukamachi, Mitani, & Kawamura, 2006), and guppy ( Poecilia reticulata ) (Kawamura et al., 2016). Conversely, dark deep‐sea fish tend to lose cone cells and corresponding opsins, whereas they possess a high proportion of rod cells, which contribute to dim light vision as in the case of lizardfish (Hope, Partridge, Dulai, & Hunt, 1997).…”

Section: Introductionmentioning

confidence: 99%

Green‐shifting ofSWS2A opsin sensitivity and loss of function ofRH2‐A opsin in flounders, genusVerasper

Kasagi

Mizusawa

Takahashi

2017

Ecology and Evolution

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We identified visual opsin genes for three flounder species, including the spotted halibut (Verasper variegatus), slime flounder (Microstomus achne), and Japanese flounder (Paralichthys olivaceus). Structure and function of opsins for the three species were characterized together with those of the barfin flounder (V. moseri) that we previously reported. All four flounder species possessed five basic opsin genes, including lws, sws1, sws2, rh1, and rh2. Specific features were observed in rh2 and sws2. The rh2‐a, one of the three subtypes of rh2, was absent in the genome of V. variegatus and pseudogenized in V. moseri. Moreover, rh2‐a mRNA was not detected in M. achne and P. olivaceus, despite the presence of a functional reading frame. Analyses of the maximum absorption spectra (λmax) estimated by in vitro reconstitution indicated that SWS2A of M. achne (451.9 nm) and P. olivaceus (465.6 nm) were blue‐sensitive, whereas in V. variegatus (485.4 nm), it was green‐sensitive and comparable to V. moseri (482.3 nm). Our results indicate that although the four flounder species possess a similar opsin gene repertoire, the SWS2A opsin of the genus Verasper is functionally green‐sensitive, while its overall structure remains conserved as a blue‐sensitive opsin. Further, the rh2‐a function seems to have been reduced during the evolution of flounders. λmax values of predicted ancestral SWS2A of Pleuronectiformes and Pleuronectidae was 465.4 and 462.4 nm, respectively, indicating that these were blue‐sensitive. Thus, the green‐sensitive SWS2A is estimated to be arisen in ancestral Verasper genus. It is suggested that the sensitivity shift of SWS2A from blue to green may have compensated functional reduction in RH2‐A.

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Section: Introductionmentioning

confidence: 99%

Green‐shifting ofSWS2A opsin sensitivity and loss of function ofRH2‐A opsin in flounders, genusVerasper

Kasagi

Mizusawa

Takahashi

2017

Ecology and Evolution

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“…; Archer and Lythgoe ; Kawamura et al. ; Watson et al. ) and guppy lens transmission data was provided by Dr. R. H. Douglas (pers.…”

Section: Methodsmentioning

confidence: 99%

“…). In addition, we also calculated absolute cone stimulation (ACS) among the different light environments, using both (i) the measured peak absorbance (λ max ) of their respective photoreceptor MSP values (; and references therein) and (ii) Kawamura et al.’s () measured peak absorbance (λ max ) values for each opsin visual pigment reconstituted in vitro ( and ).…”

Section: Methodsmentioning

confidence: 99%

“…Relative cone stimulations were estimated for each reported guppy cone type using guppy ocular media transmission and the guppy λ max values shown on the right side of this heat map (reviewed in Kawamura et al. ). The opsins predicted to be present in each guppy cone type are listed on the far right side of the heat map (Kawamura et al.…”

Section: Methodsmentioning

confidence: 99%

“…The opsins predicted to be present in each guppy cone type are listed on the far right side of the heat map (Kawamura et al. ). RCS, Relative cone stimulation.…”

Section: Methodsmentioning

confidence: 99%

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Light environment change induces differential expression of guppy opsins in a multi‐generational evolution experiment

et al. 2018

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Light environments critically impact species that rely on vision to survive and reproduce. Animal visual systems must accommodate changes in light that occur from minutes to years, yet the mechanistic basis of their response to spectral (color) changes is largely unknown. Here, we used a laboratory experiment where replicate guppy populations were kept under three different light environments for up to 8-12 generations to explore possible differences in the expression levels of nine guppy opsin genes. Previous evidence for opsin expression-light environment "tuning" has been either correlative or focused exclusively on the relationship between the light environment and opsin expression over one or two generations. In our multigeneration experiment, the relative expression levels of nine different guppy opsin genes responded differently to light environment changes: some did not respond, while others differed due to phenotypic plasticity. Moreover, for the LWS-1 opsin we found that, while we observed a wide range of plastic responses under different light conditions, common plastic responses (where the population replicates all followed the same trajectory) occurred only after multigenerational exposure to different light environments. Taken together this suggests that opsin expression plasticity plays an important role in light environment "tuning" in different light environments on different time scales, and, in turn, has important implications for both visual system function and evolution.

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Origin and adaptation of green‐sensitive (RH2) pigments in vertebrates

Yokoyama

Jia

2020

FEBS Open Bio

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One of the critical times for the survival of animals is twilight where the most abundant visible lights are between 400 and 550 nanometres (nm). Green‐sensitive RH2 pigments help nonmammalian vertebrate species to better discriminate wavelengths in this blue‐green region. Here, evaluation of the wavelengths of maximal absorption (λmaxs) of genetically engineered RH2 pigments representing 13 critical stages of vertebrate evolution revealed that the RH2 pigment of the most recent common ancestor of vertebrates had a λmax of 503 nm, while the 12 ancestral pigments exhibited an expanded range in λmaxs between 474 and 524 nm, and present‐day RH2 pigments have further expanded the range to ~ 450–530 nm. During vertebrate evolution, eight out of the 16 significant λmax shifts (or |Δλmax| ≥ 10 nm) of RH2 pigments identified were fully explained by the repeated mutations E122Q (twice), Q122E (thrice) and M207L (twice), and A292S (once). Our data indicated that the highly variable λmaxs of teleost RH2 pigments arose from gene duplications followed by accelerated amino acid substitution.

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Spectral sensitivity of guppy visual pigments reconstituted in vitro to resolve association of opsins with cone cell types

Cited by 35 publications

References 39 publications

Green‐shifting ofSWS2A opsin sensitivity and loss of function ofRH2‐A opsin in flounders, genusVerasper

Green‐shifting ofSWS2A opsin sensitivity and loss of function ofRH2‐A opsin in flounders, genusVerasper

Light environment change induces differential expression of guppy opsins in a multi‐generational evolution experiment

Origin and adaptation of green‐sensitive (RH2) pigments in vertebrates

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