Spectral Transmission and Scattering Properties of Red Blood Cells*

MacRae, R. A.; McClure, Joseph A.; Latimer, Paul

doi:10.1364/josa.51.001366

Cited by 54 publications

(26 citation statements)

References 11 publications

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“…The measured indices of refraction of HbO 2 and Hb solutions at 800 nm are 1:392 0:001 and 1:388 0:002, respectively, average SD of 1000 measurements. These values are in very close agreement with values reported in the literature, recalculated at 800 nm when appropriate [13][14][15][16][17]. In Ref.…”

supporting

confidence: 90%

Oxygen Saturation-Dependent Absorption and Scattering of Blood

et al. 2004

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supporting

confidence: 90%

Oxygen Saturation-Dependent Absorption and Scattering of Blood

et al. 2004

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“…The fitting leads to the conclusion that A c = ln(10) × E c = 0.8 at 414 nm. It is gratifying to note that an equally satisfactory fit was obtained by modeling each cluster as a sphere, and in this case A s = ln(10) × E s = 1.1 at 414 nm, which is not far from the value 3 2 × 0.8, expected on the basis of the foregoing analysis, and that these values are in fair agreement with the dimensions of a red blood cell and its decadic absorption coefficient at 414 nm, approximately 0.3/µm [12].…”

Section: Flattening In the Spectrum Of Red Blood Cellssupporting

confidence: 56%

Screening hypochromism (sieve effect) in red blood cells: a quantitative analysis

Naqvi¹

2014

Biomed. Opt. Express

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Abstract:Multiwavelength UV-visible spectroscopy, Kramers-Kronig analysis, and several other experimental and theoretical tools have been applied over the last several decades to fathom absorption and scattering of light by suspensions of micron-sized pigmented particles, including red blood cells, but a satisfactory quantitative analysis of the difference between the absorption spectra of suspension of intact and lysed red blood cells is still lacking. It is stressed that such a comparison is meaningful only if the pertinent spectra are free from, or have been corrected for, scattering losses, and it is shown that Duysens' theory can, whereas that of Vekshin cannot, account satisfactorily for the observed hypochromism of suspensions of red blood cells.

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“…The refractive index of the cell bounded haemoglobin solution was derived by the equation n solution ϭn water ϩ␤•c, where c is the haemoglobin concentration in g/100 ml and ␤ϭ0.001 942 at a wavelength of 589 nm. 21 OPTICAL PROPERTIES OF BLOOD ber density of 8.33ϫ10 11 L −1 which was related to hctϭ7.5% at 300 mosmol/L and which was kept constant at all osmolarities. Figure 9 shows the results of the Mie calculations for s and g compared with the measured data.…”

Section: Osmolaritymentioning

confidence: 99%

Optical Properties of Circulating Human Blood in the Wavelength Range 400–2500 nm

et al. 1999

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Knowledge about the optical properties μa,μs, and g of human blood plays an important role for many diagnostic and therapeutic applications in laser medicine and medical diagnostics. They strongly depend on physiological parameters such as oxygen saturation, osmolarity, flow conditions, haematocrit, etc. The integrating sphere technique and inverse Monte Carlo simulations were applied to measure μa,μs, and g of circulating human blood. At 633 nm the optical properties of human blood with a haematocrit of 10% and an oxygen saturation of 98% were found to be 0.210±0.002 mm-1 for μa,77.3±0.5 mm-1 for μs, and 0.994±0.001 for the g factor. An increase of the haematocrit up to 50% lead to a linear increase of absorption and reduced scattering. Variations in osmolarity and wall shear rate led to changes of all three parameters while variations in the oxygen saturation only led to a significant change of the absorption coefficient. A spectrum of all three parameters was measured in the wavelength range 400-2500 nm for oxygenated and deoxygenated blood, showing that blood absorption followed the absorption behavior of haemoglobin and water. The scattering coefficient decreased for wavelengths above 500 nm with approximately λ-1.7; the g factor was higher than 0.9 over the whole wavelength range. © 1999 Society of Photo-Optical Instrumentation Engineers.

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Spectral Transmission and Scattering Properties of Red Blood Cells*

Cited by 54 publications

References 11 publications

Oxygen Saturation-Dependent Absorption and Scattering of Blood

Oxygen Saturation-Dependent Absorption and Scattering of Blood

Screening hypochromism (sieve effect) in red blood cells: a quantitative analysis

Optical Properties of Circulating Human Blood in the Wavelength Range 400–2500 nm

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