Spectroelectrochemical Sensing of Pyrene Metabolites 1-Hydroxypyrene and 1-Hydroxypyrene-glucuronide

Wilson, Robert A.; Seliskar, Carl J.; Talaska, Glenn; Heineman, William R.

doi:10.1021/ac200161s

Cited by 21 publications

(13 citation statements)

References 34 publications

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“…From a quantitative point of view, the most used SEC techniques are UV/Vis absorption SEC , fluorescence SEC and Raman SEC . For this reason, and taking into account the objectives of this review, these three techniques are those that will be described in more detail below.…”

Section: Whatmentioning

confidence: 99%

“…The main advantage of SEC for analysis is the electrochemical transformation of the analytes, or of other compounds that affect the analyte during an experiment, yielding a change in the optical signal during the measurement . Therefore, in PL‐SEC is not usual to obtain the excitation spectra and the emission spectra in the same experiment.…”

Section: Howmentioning

confidence: 99%

“…The thin‐layer approach was also used to quantitatively distinguish between 1‐hydroxypyrene and 1‐hydroxypyrene‐glucuronide . These polycyclic aromatic hydrocarbon biomarkers exhibit overlapped fluorescence spectra and can be determined using PL‐SEC without the need of a separation step.…”

Section: Advantages In Usementioning

confidence: 99%

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Spectroelectrochemical Sensing: Current Trends and Challenges

et al. 2019

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Spectroelectrochemistry (SEC) has been used for more than 50 years, but this set of techniques has not been widely used for quantitative analysis. For many years, no commercial instruments were available, which made very difficult to spread the use of SEC. Nowadays, only the creativity of the researchers is required to exploit the capabilities of SEC. This review is written with the aim of showing the potential of SEC, mainly for analytical chemistry. Here, we explain what SEC is, how analytical responses can be obtained, why these techniques are useful for sensors, with a brief description of its advantages in use, and, finally, we try to show the challenges that must be addressed in the next years. SEC can resolve interesting analytical problems using the high amount of data provided by this intrinsic trilinear technique. Given the quantitative analysis point of view of this review, the discussion of the SEC techniques is focused on UV/Vis absorption, photoluminescence and Raman SEC.

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Section: Whatmentioning

confidence: 99%

Section: Howmentioning

confidence: 99%

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Spectroelectrochemical Sensing: Current Trends and Challenges

et al. 2019

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“…54 Approximately 80% of pyrene metabolites excreted in urine are 1-PyOglu. A common approach to assess human PAH exposure is to first convert all metabolite to 1-PyOH.…”

Section: Polycyclic Aromatic Hydrocarbonsmentioning

confidence: 99%

Spectroelectrochemistry as a strategy for improving selectivity of sensors for security and defense applications

Heineman¹,

Seliskar²,

Morris³

et al. 2012

SPIE Proceedings

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Spectroelectrochemistry provides improved selectivity for sensors by electrochemically modulating the optical signal associated with the analyte. The sensor consists of an optically transparent electrode (OTE) coated with a film that preconcentrates the target analyte. The OTE functions as an optical waveguide for attenuated total reflectance (ATR) spectroscopy, which detects the analyte by absorption. Alternatively, the OTE can serve as the excitation light for fluorescence detection, which is generally more sensitive than absorption. The analyte partitions into the film, undergoes an electrochemical redox reaction at the OTE surface, and absorbs or emits light in its oxidized or reduced state. The change in the optical response associated with electrochemical oxidation or reduction at the OTE is used to quantify the analyte. Absorption sensors for metal ion complexes such as [Fe(CN) 6 ] 4-and [Ru(bpy) 3 ] 2+ and fluorescence sensors for [Ru(bpy) 3 ] 2+ and the polycyclic aromatic hydrocarbon 1-hydroxypyrene have been developed. The sensor concept has been extended to binding assays for a protein using avidin-biotin and 17β-estradiol-anti-estradiol antibodies. The sensor has been demonstrated to measure metal complexes in complex samples such as nuclear waste and natural water. This sensor has qualities needed for security and defense applications that require a high level of selectivity and good detection limits for target analytes in complex samples. Quickly monitoring and designating intent of a nuclear program by measuring the Ru/Tc fission product ratio is such an application.

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“…Identification and profiling of metabolites of OH-PAHs are therefore of critical importance to explore and elucidate metabolic pathways of PAHs in human body. Previous studies have revealed presence of some metabolites of OH-PAHs, such as glucuronides and sulfates in human urine [37][38][39][40][41][42]. However, detailed identification and profiling of OH-PAHs metabolites in human body have not been reported yet.…”

Section: Introductionmentioning

confidence: 99%