Spectrofluorimetric analysis of certain 4-quinolone in pharmaceuticals and biological fluids

Rizk, Mohamed; Belal, F.; Ibrahim, F.; Ahmed, Somia B.; El‐Enany, N.

doi:10.1016/s0031-6865(00)00025-x

Cited by 30 publications

(21 citation statements)

References 17 publications

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“…Methods for the determination of QNs have been reviewed [1][2][3]. Numerous biological and chemical techniques, such as microbiological assay [4], spectrophotometry [5], fluorimetry [6][7][8], high-performance liquid chromatography [9,10], and flow-injection chemiluminescence methods [11], have been reported.…”

Section: Introductionmentioning

confidence: 99%

Determination of quinolone antibiotics in urine by capillary electrophoresis with chemiluminescence detection

Liu¹,

Jia²,

Tian³

2008

J of Separation Science

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A novel and simple method is presented for the determination of norfloxacin, ciprofloxacin, and ofloxacin by capillary electrophoresis with chemiluminescence detection. This method is based on the enhancing effect of quinolones on the chemiluminescence reaction of the Ce(SO(4))(2)-Ru(bpy)(3)(2+)-HNO(3) system. Three quinolones were successfully separated and detected under optimum conditions. The obtained detection limits were 2.3x10(-7) mol/L, 5.2x10(-8) mol/L, and 7.8x10(-8) mol/L for ciprofloxacin, norfloxacin, and ofloxacin, respectively. The RSD of migration time and peak area were less than 1.8 and 3.8% (n = 5), respectively. The applicability of the proposed method was illustrated in the determination of ofloxacin in eye drops and of norfloxacin in human urine samples, and the monitoring of pharmacokinetics for norfloxacin.

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Section: Introductionmentioning

confidence: 99%

Determination of quinolone antibiotics in urine by capillary electrophoresis with chemiluminescence detection

Liu¹,

Jia²,

Tian³

2008

J of Separation Science

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“…Various spectrophotometric methods were described for determination of CIP, ENR and PEF [12] , NOR [13] and LEV [14] by charge-transfer complex formation with chloranilic acid, tetracyanoquinodimethane and tetracyanoethylene. In addition, several techniques have been reported for their determination such as spectrophotometry [15,16] , fluorimetry [17][18][19] , polarography [20] , voltametry [21] and capillary electrophoresis [22] . HPLC methods generally require complex and expensive equipment, provision for use and disposal of solvents, labor-intensive sample preparation procedure and personal skilled in chromatographic techniques.…”

Section: Introductionmentioning

confidence: 99%

Spectrofluorimetric Determination of Certain Fluoroquinolones Through Charge Transfer Complex Formation

Salem¹,

Fada²,

Khater³

2007

American J. of Pharmacology and Toxicology

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A highly sensitive spectrofluorimetric method was developed for the first time, for the analysis of ten fluoroquinolones (FQs) antibacterials, namely amifloxacin (AMI), ciprofloxacin (CIP), difloxacin (DIF), enoxacin (ENO), enrofloxacin (ENR), lomefloxacin (LOM), levofloxacin (LEV), norfloxacin (NOR), ofloxacin (OFL) and pefloxacin (PEF) in their pharmaceutical dosage forms or in biological fluids through charge transfer (CT) complex formation with bromanil (BRO). The BRO was found to react with these drugs to produce stable complexes and the fluorescence intensity of the complexes was greatly enhanced. The formation of such complexes was also confirmed by ultravioletvisible measurements. The different experimental parameters that affect the fluorescence intensity were carefully studied. At the optimum reaction conditions, the drug-BRO complexes showed excitation maxima ranging from 275 to 290 nm and emission maxima ranging from 450 to 470 nm. Rectilinear calibration graphs were obtained in the concentration range 0.02 to 3.1 µg.mL -1 for the studied drugs. The method has been successfully applied to determine their pharmaceutical dosage forms with good precision and accuracy compared to official and reported methods as revealed by tand F-tests. They are also applied for the determination of studied drugs in human urine samples.

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“…Although, limited literatures have been reported for the determination of gemifloxacin spectrofluorometrically [10, 11]. Micelle-enhanced spectrofluorimetric method has been reported for determination of many drugs [12–17] due to the ability of micelle formation to increase the fluorescence intensity of the weakly fluorescent compounds. Moreover, these methods introduced sensitive and nonpollutant methodology, since no organic solvents were used.…”

Section: Introductionmentioning

confidence: 99%

Two Validated Spectrofluorometric Methods for Determination of Gemifloxacin Mesylate in Tablets and Human Plasma

Atia

Mahmoud

El-Shabouri

et al. 2013

International Journal of Analytical Chemistry

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Two new, sensitive, and selective spectrofluorometric methods were developed for the determination of gemifloxacin mesylate (GFX) in tablets and spiked human plasma. Method A was based on measurement of the enhanced fluorescence spectral behaviour of GFX in a sodium dodecyl sulphate (SDS) micellar system. In aqueous solution of acetate buffer pH 5.5, the fluorescence intensity of GFX was greatly enhanced about tenfold in the presence of SDS. The fluorescence intensity was measured at 402 nm after excitation at 274 nm. Method B was based on Hantzsch condensation reaction between the primary amino group of GFX with acetylacetone and formaldehyde in acetate buffer of pH 3.5 yielding a highly yellow fluorescent derivative. The reaction of GFX with acetylacetone-formaldehyde system solution resulted in bathochromic shift of both emission (476 nm) and excitation (420 nm) wavelengths. The fluorescence intensity was directly proportional to the concentration over the range 10–1000 ng/ml and 100–2000 ng/ml for method A and B, respectively. The proposed methods were applied successfully for determination of GFX in its tablets and spiked plasma. Therefore, these methods can be considered of real interest for reliable and practical quality control analysis of GFX.

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Spectrofluorimetric analysis of certain 4-quinolone in pharmaceuticals and biological fluids

Cited by 30 publications

References 17 publications

Determination of quinolone antibiotics in urine by capillary electrophoresis with chemiluminescence detection

Determination of quinolone antibiotics in urine by capillary electrophoresis with chemiluminescence detection

Spectrofluorimetric Determination of Certain Fluoroquinolones Through Charge Transfer Complex Formation

Two Validated Spectrofluorometric Methods for Determination of Gemifloxacin Mesylate in Tablets and Human Plasma

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