Spectrofluorimetric determination of vigabatrin and gabapentin in urine and dosage forms through derivatization with fluorescamine

Preparation and characterization of cationic Pluronic for surface modification and functionalization of polymeric drug delivery nanoparticles

“…The transformation of hydroxyl groups of Pluronic into amine groups was detected with the aid of the fluorescamine assay, which is a sensitive method for the surveying of primary amines [33][34][35]. Samples were prepared in 0.1 M borate buffer at pH 9 with a polymer concentration of 0.2 g/L.…”

A Simple High-Throughput Method for Determination of Antiepileptic Analogues of γ-Aminobutyric Acid in Pharmaceutical Dosage Forms Using Microplate Fluorescence Reader

“…1) The analytical method development for PGB, GBP and VGB determination is challenging since these molecules have no significant UV or visible absorption. Several derivatization methods have been proposed [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19] and used for their determination in bulk or pharmaceutical dosage forms, plasma, serum, and urine. These methods employed derivatization reagents such as fluorescamine, [2][3][4][17][18][19] 7-chloro-4-nitrobenzofurazan, [5][6][7] 1-fluoro-2,4-dinitrobenzene, 8) o-phtaldialdehyde, 9,10) 2,4,6-trinitrobenzene sulfonic acid, 11,12) and 9-fluorenylmethylchloroformate.…”

“…2,[5][6][7]17,19) Moreover, HPLC 3,[8][9][10][11][12][13][14]18) and capillary electrophoresis (CE) methods 4) using precolumn derivatization with either UV or fluorescent detector were also used.…”

“…In addition method should meet validation criteria in terms of precision, accuracy, robustness etc., and most of all it has to offer highthroughput. So far developed methods for determination of PGB, 3,5,17,19) GBP, 2,7,8,15,16,18,[20][21][22][23][24] and VGB 2,6,7,18,25) use from a few µL to approximately 1 mL of sample. The sample preparation is the time-consuming step in derivatization methods and in case of PGB, GBP, and VGB derivatization can take from few seconds to 50 min.…”

Optimization of an HPLC Method for Determination of Gabapentin in Dosage Forms through Derivatization with 1-Fluoro-2,4-dinitrobenzene

“…In one of the methods, spectrofluorometric determination of gabapentin was reported after derivatization with fluorescamine. 17) In another study, colorimetric determination of gabapentin was studied based on the reaction with vanillin and ninhydrin. 18) In these cases, the derivatization condition was time consuming and the stability of the reaction products depends on experimental conditions such as pH, temperature and reaction time.…”